全文获取类型
收费全文 | 1021篇 |
免费 | 28篇 |
国内免费 | 11篇 |
学科分类
数理化 | 1060篇 |
出版年
2023年 | 17篇 |
2022年 | 25篇 |
2021年 | 23篇 |
2020年 | 24篇 |
2019年 | 17篇 |
2018年 | 13篇 |
2017年 | 16篇 |
2016年 | 24篇 |
2015年 | 35篇 |
2014年 | 30篇 |
2013年 | 49篇 |
2012年 | 64篇 |
2011年 | 93篇 |
2010年 | 53篇 |
2009年 | 95篇 |
2008年 | 41篇 |
2007年 | 66篇 |
2006年 | 45篇 |
2005年 | 46篇 |
2004年 | 60篇 |
2003年 | 31篇 |
2002年 | 35篇 |
2001年 | 14篇 |
2000年 | 24篇 |
1999年 | 12篇 |
1998年 | 21篇 |
1997年 | 11篇 |
1996年 | 18篇 |
1995年 | 8篇 |
1994年 | 5篇 |
1993年 | 14篇 |
1992年 | 5篇 |
1991年 | 6篇 |
1990年 | 5篇 |
1989年 | 4篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有1060条查询结果,搜索用时 15 毫秒
111.
电化学免疫传感器在食品安全检测中的应用进展 总被引:1,自引:0,他引:1
介绍了3种电化学免疫传感器的组装、构建,着重评论了近十年来其在食品安全检测中的应用研究,并对其前景进行了展望(引用文献30篇)。 相似文献
112.
高效液相色谱-串联质谱法测定食品中维生素D_2和维生素D_3 总被引:1,自引:0,他引:1
提出了食品中维生素D2和维生素D3的高效液相色谱-串联质谱分析方法。食品样品经氢氧化钾皂化后乙醚萃取,所得有机相经无水硫酸钠除水后蒸发至干。用1 mL乙醇溶解后经Agilent Zorbax XDB C18色谱柱(2.1 mm×50 mm,3.5μm)分离,用甲-醇10 mmol·L-1乙酸铵溶液(90+10)的混合溶液洗脱,采用电喷雾正离子模式多反应监测。维生素D2和维生素D3的线性范围均为50~500μg·L-1,检出限(3S/N)均为10μg·L-1。方法应用于测定奶粉和酸酸乳中维生素D2和维生素D3,回收率在76.8%~83.9%之间。 相似文献
113.
114.
Review: Authentication and traceability of foods from animal origin by polymerase chain reaction-based capillary electrophoresis 总被引:2,自引:0,他引:2
This work presents an overview of the applicability of PCR-based capillary electrophoresis (CE) in food authentication and traceability of foods from animal origin. Analytical approaches for authenticating and tracing meat and meat products and fish and seafood products are discussed. Particular emphasis will be given to the usefulness of genotyping in food tracing by using CE-based genetic analyzers. 相似文献
115.
Ionic liquid–salt aqueous two-phase flotation (ILATPF) is a novel, green, non-toxic and sensitive samples pretreatment technique. ILATPF coupled with high-performance liquid chromatography (HPLC) was developed for the analysis of chloramphenicol, which combines ionic liquid aqueous two-phase system (ILATPS) based on imidazolium ionic liquid (1-butyl-3-methylimidazolium chloride, [C4mim]Cl) and inorganic salt (K2HPO4) with solvent sublation. In ILATPF systems, phase behaviors of the ILATPF were studied for different types of ionic liquids and salts. The sublation efficiency of chloramphenicol in [C4mim]Cl–K2HPO4 ILATPF was influenced by the types of salts, concentration of K2HPO4 in aqueous solution, solution pH, nitrogen flow rate, sublation time and the amount of [C4mim]Cl. Under the optimum conditions, the average sublation efficiency is up to 98.5%. The mechanism of ILATPF contains two principal processes. One is the mechanism of IL–salt ILATPS formation, the other is solvent sublation. This method was practical when applied to the analysis of chloramphenicol in lake water, feed water, milk, and honey samples with the linear range of 0.5–500 ng mL−1. The method yielded limit of detection (LOD) of 0.1 ng mL−1 and limit of quantification (LOQ) of 0.3 ng mL−1. The recovery of CAP was 97.1–101.9% from aqueous samples of environmental and food samples by the proposed method. Compared with liquid–liquid extraction, solvent sublation and ionic liquid aqueous two-phase extraction, ILATPF can not only separate and concentrate chloramphenicol with high sublation efficiency, but also efficiently reduce the wastage of IL. This novel technique is much simpler and more environmentally friendly and is suggested to have important applications for the concentration and separation of other small biomolecules. 相似文献
116.
Ferrer C Lozano A Agüera A Girón AJ Fernández-Alba AR 《Journal of chromatography. A》2011,1218(42):7634-7639
During recent years matrix effects in liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) have quickly become a major concern in food analysis. The phenomenon of ion suppression can lead to errors in the quantification of the analytes of interest, as well as can affect detection capability, precision, and accuracy of the method. Sample dilution is an easy and effective method to reduce interfering compounds, and so, to diminish matrix effects. In this work, matrix effects of 53 pesticides in three different matrices (orange, tomato and leek) were evaluated. Several dilutions of the matrix were tested in order to study the evolution of signal suppression. Dilution of the extracts led to a reduction of the signal suppression in most of the cases. A dilution factor of 15 demonstrated to be enough to eliminate most of the matrix effects, opening the possibility to perform quantification with solvent based standards in the majority of the cases. In those cases where signal suppression could not be reduced, a possible solution would be to use stable isotope-labelled internal standards for quantification of the problematic pesticides. 相似文献
117.
A new method based on matrix solid phase dispersion-capillary electrophoresis with electrochemiluminescence detection (MSPD-CE-ECL) has been developed for the simultaneous determination of three kinds of phenylurea herbicides (PHUs). Poly-β-cyclodextrin (poly-β-CD) was used as an additive in the running buffer to improve the separation of three analytes. The conditions for CE separation, ECL detection and effect of poly-β-CD were investigated in detail. Under the optimal conditions, three kinds of herbicides (isoproturon, linuron and diuron) were well separated and detected within 8 min. The linear ranges of the standard solution for isoproturon and linuron were 1-300 μg L(-1) with a detection limit (S/N=3) of 0.1 μg L(-1), and for diuron was 2-500 μg L(-1) with a detection limit of 0.2 μg L(-1). The average recoveries were in the range of 86.9-102.8%, and all relative standard deviation of the migration time and the ECL intensity in intraday and interday were less than 9.0%. The proposed method was also applied to the determination of three kinds of herbicides in green vegetable and rice samples with recoveries in the range from 78.1 to 93.8%. 相似文献
118.
The use of hydrophilic interaction liquid chromatography (HILIC) in food analysis in the last decade is reviewed. The HILIC mechanism is briefly discussed, but main emphasis is put on the use of HILIC for separation of different food matrices. The food matrices are divided into food of animal origin and related products, vegetables, fruits and related compounds, and other food-related matrices. A list on important applications is provided for each category including experimental conditions and a brief summary of the results. The 100 references included will provide the reader a comprehensive overview and insight into HILIC applications to food analysis. 相似文献
119.
Analytical methods applied to the determination of pesticide residues in foods of animal origin. A review of the past two decades 总被引:5,自引:0,他引:5
LeDoux M 《Journal of chromatography. A》2011,1218(8):1021-1036
Pesticides are widely used in agriculture and can be transferred to animals in a number of ways. Consequently, reliable analytical methods are required to determine pesticide residues in foods of animal origin. The present review covers published methods and research articles (1990-2010) in which pesticide residues have been extracted from meat and meat products, milk and dairy products, fish and seafood, and eggs, then cleaned up, and isolated by chromatographic techniques to be identified and quantified by various detection methods. Recovery rates, quantification limits, the matrix effect and related parameters have all been considered. Lastly, future developments in this field are outlined. 相似文献
120.
《Operations Research Letters》2019,47(6):537-541
We investigate optimization models for meal delivery that do not involve routing. We focus on the simplest case, with a single restaurant and a single courier, and reduce it to the throughput maximization single machine scheduling problem, with release times and bounded slacks. In the meal delivery setting, slacks are small for QoS purposes and we indeed show that the scheduling problem can be solved in polynomial time when slacks are bounded. We discuss extensions to more couriers and restaurants. 相似文献