Effects of“Moxibustion Serum”on Proliferation and Phenotypes of Tumor Infiltrating Lymphocytes

Tumor infiltrating lymphocytes (TIL) were cultured with “moxibustion serum” (MS),and the results wereexamined by flow cytometry.The results indicated that MS could enhance the proliferation of TIL,accelerate it to reach the exponential growth phase,and assist recombinant interleukin 2 (rIL-2) to enhancesuccessively the percentage of CD_3~+ positive cells,maintain the number of CD_4~+ positive T cells,promotegreatly the percentage of CD_8~+ positive T cells among TILs,and reverse the CD_4~+/CD_8~+ ratio.Suchcooperative effects rely on relative specificity of acupoints.It is suggested that MS is beneficial to thegrowth of TIL both in the aspects of proliferation and phenotypes.     

在针刺镇痛过程中脑内GABA的作用及其对5—HT代谢的影响(科研简讯)

荷包牡丹碱为γ-氨基丁酸(GABA)的竞争性拮抗剂,可以选择性对抗GABA与受体的结合,是目前研究中枢神经生理的重要工具。为了进一步证明中枢GABA能系统在针刺镇痛过程中的作用及其对5-羟色胺(5-HT)代谢的影响,我们观察了大鼠腹腔注射荷包牡丹碱对电针镇痛效应及其脑内5-HT和他的主要代谢产物5-羟吲哚乙酸(5-HIAA)含量变化的影响。每次实验选取四只体重相似性别一致的大鼠,随机分为四组;(1)对照组,只注射生理盐水,(2)电针组,注射生理盐水后20分钟电针;(3)荷包牡丹碱组,只注射荷包牡丹碱;(4)荷包牡丹碱+电针组,注射荷包牡丹碱后20分钟电针。除电针和药物注射外,其它处理相同。每组动物共12只。     

中枢儿茶酚胺系统在针刺调节肾上腺皮质功能中的作用

电针可以引起家兔血清皮质醇含量升高。侧脑室注射６－羟多巴胺（６－ＯＨＤＡ）对电针升高血清皮质醇含量有促进作用，而注射二羟基苯丝氨酸（ＤＯＰＳ）有抑制作用。提示脑内儿茶酚胺（ＣＡ），特别是去甲肾上腺素（ＮＥ）可能参与电针对肾上腺皮质激素分泌的调节。     

Effects of Medicinal Cake-Separated Moxibustion on Plasma 6-Keto-PGF1α and TXB2 Contents in the Rabbit of Hyperlipemia

Hyperlipemia rabbit models established with high cholesterol and fat diet were treated vith directmoxibustion and medicinal cake-separated moxibustion. The post-treatment plasma 6-keto-prostaglandin F_(1α)(6-keto-PGF_(1α)) and thromboxane B2 (TXB_2) contents were determined by radioimmunoassay. Resultsindicated that the plasma 6-keto-PGF_(1α) content significantly increased,the TXB_2 level decreased (P<0.05)and the TXB_2 /6-keto-PGF_(1α) ratio also decreased (P<0.01) in the medicinal cake-separated moxibustiongroup as compared with those in the model group respectively,but there was no significant differencebetween the medicinal cake-separated moxibustion group and the direct moxibustion group (P>0.05),suggesting that both the medicinal cake-separated moxibustion and direct moxibustion can regulate theplasma 6-keto-PGF_(1α) and TXB_2 contents,and the TXB_2/6-keto-PGF_(1α) ratio with similar actions,and have acertain protective action on endothelial cells of the aorta in the rabbit of hyperlipemia     

电针对急性心肌缺血大鼠血浆中ET、NO和CGRP含量的影响

目的 :观察电针双侧“内关 间使”穴对急性实验性心肌缺血大鼠血浆中内皮素 (ET)、一氧化氮 (NO)和降钙素基因相关肽 (CGRP)含量的影响 ,探讨针刺改善急性心肌缺血及心包经穴与心脏相关联系的机制。方法 :结扎冠状动脉前降支造成心肌缺血 ,血浆中的ET和CGRP含量用放射免疫方法测定 ,NO含量用硝酸还原酶法测定。结果 :急性心肌缺血大鼠血浆中ET含量明显降低 ,NO含量显著升高 ;电针“内关 间使”穴后血浆ET含量显著升高 ,NO含量显著下降 ,大鼠急性心肌缺血及电针“内关 间使”穴对血浆CGRP含量没有明显影响。结论 :ET和NO可能参与电针改善心肌缺血的作用以及心包经穴与心脏相关联系。     

EFFECTS OF BICUCULLINE ON THE CHANGES OF PAIN THRESHOLD AND SOME NEUROTRANSMITTERS OF BRAIN IN RATS INDUCED BY EAA

After electroacupuncture(EA)for 25 minutes atthe bilateral Shousanli(LI 10)and Huantiao(GB30)acupoints of rats,5-hydroxytryptamine(5-HT)contents in medulla oblongata plus pons andmidbrain,LEK level in striatum and DA concentra-tions in brain stem and diencephalon markedly in-creased with an elevation of pain threshold.How-ever,a previous intraperitoneal injection of bicucul-line,a receptor blocker of γ-aminobutyric acid(GABA),not only partially blocked the effect ofEAA,but also abolished the increase of 5-HT in themedulla oblongata plus pons and the midbrain,LEKin the striatum,and DA in the brain stem and thediencephalon.Also,5-HT in the striatum and LEKin the medulla oblougata plus pons were enhancedand 5-HIAA in hypothalamus and cortex were re-duced significantly after EA.These results suggest that GABA receptor playsan importent role in EAA,which may be partiallymediated via the central 5-HT,LEK and DA systemin the brain.     

不同时辰电针大鼠“环跳”穴对血浆cAMP、cGMP水平的影响

<正> “子午流注”针灸疗法是中医颇具特色的治疗手段,临床实践表明疗效较好。用现代时辰生物学方法对这一独特疗法加以深入研究是有重要意义的。在许多细胞的生理活动中,cAMP,cGMP 是重要的第二信使,二者在细胞内的含量及其比例与细胞的功能状态有密切关系。已有文献报道人血浆 cAMP 水平存在近日节律性变化;另有报道表明血浆