Targeting EGFR-overexpressing tumor cells using Cetuximab-immunomicelles loaded with doxorubicin and superparamagnetic iron oxide

Epidermal growth factor receptor (EGFR), a cellular transmembrane receptor, plays a key role in cell proliferation and is linked to a poor prognosis in various human cancers. In this study, we constructed Cetuximab-immunomicelles in which the anti-EGFR monoclonal antibody was linked to poly(ethylene glycol)-block-poly(?-caprolactone) (PEG–PCL) nanomicelles that were loaded with doxorubicin (DOX) and superparamagnetic iron oxide (SPIO). The specific interactions between EGFR-overexpressing tumor cells (A431) and immunomicelles were observed using confocal laser scanning microscopy (CLSM) and flow cytometry. Furthermore, the capacity of transporting SPIO into tumor cells using these immunomicelles was evaluated with a 1.5 T clinical magnetic resonance imaging (MRI) scanner. It was found that the acquired MRI T2 signal intensity of A431 cells that were treated with the SPIO-loaded and antibody-functionalized micelles decreased significantly. Using the thiazolyl blue tetrazolium bromide (MTT) assay, we also demonstrated that the immunomicelles inhibited cell proliferation more effectively than their nontargeting counterparts. Our results suggest that Cetuximab-immunomicelles are a useful delivery vehicle for DOX and SPIO to EGFR-overexpressing tumor cells in vitro and that Cetuximab-immunomicelles can serve as a MRI-visible and targeted drug delivery agent for better tumor imaging and therapy.     

我院医疗设备管理系统的设计与开发

目的利用自建的医疗设备管理系统软件,提高医院医疗设备维修保养以及检定工作的效率,并为医院提供一个方便快、捷的设备信息查询平台。方法采用ActiveXDataOhjects应用程序接口技术开发了适合我院使用的医疗设备维修保养以及检定信息的计算机管理系统;使用ACCESS2003构件建立全院医疗设备的数据库、使用开发环境软件制作系统用户界面,使界面更便于操作。结果该系统已成功应用到我院医疗设备管理工作中,并取得了良好的效果。结论该系统的应用提高了工作效率,为医院设备的规范管提供了极大的便利。     

自拟补肾促排卵汤治疗排卵障碍性不孕86例分析

目的:观察补肾方药治疗排卵障碍性不孕的临床疗效。方法:补肾促排卵汤临床结合辨证加减与西药克罗米芬联合应用为观察组86例,单独应用克罗米芬为对照组44例,进行服药前后自身对照和两组间的比较。结果:中西药结合应用的观察组总妊娠率(治愈)为61.63%(53/86),显著性高于对照组的43.18%(19/44)(P﹤0.05),同时具有调节月经周期、促进卵泡和内膜生长发育的整体调节效果。结论:补肾方药治疗排卵障碍性不孕,通过调整肾—天癸—冲任—胞宫轴功能,体现的肾主生殖的中医思想。     

杞菊地黄丸联合倪福达治疗原发性高血压30例观察

目的:观察杞菊地黄丸联合倪福达治疗原发性高血压的临床疗效。方法:治疗组30例用杞菊地黄丸联合倪福达治疗,对照组30例用卡托普利治疗,两组均治疗28天。结果:治疗后两组收缩压、舒张压、症状及心率下降程度比较差异均有统计学意义(P<0.01),治疗组疗效优于对照组。结论:杞菊地黄丸联合倪福达治疗原发性高血压有明显降压效果,优于卡托普利。     

从技术至上走向人文关怀——现代医学模式在实践中转化的必要条件

现代医学模式在实践中的转化有赖于医学对人们心理和社会因素的关怀，而传统生物医学模式下形成的技术至上观念防碍了关怀的实现，进而也给现代医学模式的转化造成了困难。因此，在医学中倡导人文关怀是必要的，从技术至上走向人文关怀将成为现代医学模式在实践中转化的必要条件。医学中的人文关怀不仅是对人的关怀，也包括对技术的关怀，是在此基础上对全人类的关怀。     

聚焦超声在脑部疾病的应用进展

血脑屏障（BBB）阻碍多数治疗药物进入脑组织,导致临床诊治脑部疾病、特别是中枢神经系统疾病困难。借助微泡造影剂,聚焦超声可以无创、重复开放BBB。近年来国内外逐渐开展了聚焦超声治疗脑部疾病的实验和临床研究。本文就聚焦超声在脑部疾病的研究进展进行综述。     

Testing Different Combinations of Acoustic Pressure and Doses of Quinolinic Acid for Induction of Focal Neuron Loss in Mice Using Transcranial Low-Intensity Focused Ultrasound

The goal of this study was to test different combinations of acoustic pressure and doses of quinolinic acid (QA) for producing a focal neuronal lesion in the murine hippocampus without causing unwanted damage to adjacent brain structures. Sixty male CD-1 mice were divided into 12 groups that underwent magnetic resonance-guided focused ultrasound at high (0.67 MPa), medium (0.5 MPa) and low (0.33 MPa) acoustic peak negative pressures and received QA at high (0.012 mmol), medium (0.006 mmol) and low (0.003 mmol) dosages. Neuronal loss occurred only when magnetic resonance-guided focused ultrasound with adequate acoustic power (0.67 or 0.5 MPa) was combined with QA. The animals subjected to the highest acoustic power had larger lesions than those treated with medium acoustic power, but two mice had evidence of bleeding. When the intermediate acoustic power was used, medium and high dosages of QA produced lesions larger than those produced by the low dosage.     

胆总管十二指肠球部异位开口的诊断与治疗

目的 探讨胆总管十二指肠球部异位开口的诊断与治疗.方法 回顾性分析2006年1月至2013年12月山东省滨州市人民医院收治的3例和天津市人民医院收治的9例胆总管十二指肠球部异位开口患者的临床资料.其中7例合并胆总管结石,5例合并胆总管末端狭窄.分析患者的病史和临床表现.检测血常规及血清肝功能指标.行ERCP检查并治疗.采用门诊和电话方式进行随访,随访时间截至2014年8月.结果 6例患者有反复发作的胆管炎病史,2例患者有反复发作的十二指肠溃疡病史.12例患者均有右季肋区疼痛.7例患者有发热寒战、皮肤巩膜黄染、右季肋区压痛.11例患者ALP和GGT升高,8例TBil和DBiL升高,7例WBC计数升高.(1)ERCP检查:12例患者十二指肠第二、三段均无乳头状开口结构.十二指肠球部后壁有略高于周围黏膜表面的裂缝样开口,无典型的主乳头结构,周围黏膜粗糙、糜烂,胆汁自开口间歇性流出.5例患者可见不同程度的十二指肠溃疡.3例患者可见十二指肠球部变形.12例患者均插管成功.(2)胆道造影检查:12例患者胆总管末端进入十二指肠球部段均逐渐变细并呈镰刀状钩形结构.10例肝内外胆管扩张;7例胆总管结石;5例胰管显影,且开口于十二指肠球部.12例患者中,8例成功施行球囊扩张术(5例胆总管末端狭窄,3例胆总管结石);3例因结石直径＞1.5cm,且胆总管十二指肠球部异位开口狭窄,行胆总管探查取石联合胆肠吻合术;1例ERCP取石失败,患者拒绝手术,行PTC经胆道镜取出胆总管结石.术后无出血、胰腺炎等并发症发生.3例患者行球囊扩张术后胆管炎表现未缓解,2例术后胆管炎复发,均行Roux-en-Y胆肠吻合术后治愈;这5例患者平均开腹手术时间为85 min(60～ 150 min),平均术后住院时间为10 d(8～14 d).12例患者术后均获得随访,中位随访时间为38个月(8 ～ 90个月).随访期间,10例患者健康生存,无胆管炎、胆道结石等复发;其余2例患者分别于术后2个月和14个月胆管炎复发,其中1例伴胆总管泥沙样结石复发,均再次入院行Roux-en-Y胆肠吻合术后治愈,后期随访无复发.结论 反复发作的胆管炎、十二指肠溃疡病史,右季肋区疼痛、皮肤巩膜黄染、肝功能异常,ERCP检查十二指肠球部裂隙样开口并有胆汁流出,胆道造影检查见胆总管末端进入十二指肠球部段逐渐变细,呈镰刀状钩形结构是胆总管十二指肠球部异位开口的临床特征.该病的治疗以针对伴发疾病为主.     

脑胶质瘤治疗后假性进展与复发的影像学鉴别研究进展

脑胶质瘤源自于神经上皮细胞,是颅内最常见的原发性肿瘤,其治疗方案为手术切除及后续的同步放化疗,但多达30%的患者会出现新的磁共振成像内的强化病变,这可能不是早期肿瘤进展(early progression,EP),而是放化疗导致的假性进展(pseudo-progression,PSP)。假性进展由于病变较轻且预后较好,只需对其进行对症治疗,而肿瘤复发时临床医生对其处理方式与假性进展不同,复发可能要选择再次手术或者使用放化疗等其他治疗方式,因此,如果胶质瘤的假性进展与复发被误诊,患者的最佳治疗时机将会受到耽误。常规磁共振成像不可能可靠地区分PSP和EP,目前一些更先进的成像方法有望对其进行准确鉴别。     

A new modification method for graphite felt electrodes in a MV/4-HO-TEMPO flow battery

Using graphite felt as the support body, reduced graphene oxide (rGO) is grown on the surface of carbon fibers by the hydrothermal reduction method, and the modified graphite felt was used as an electrode material and studied in a methyl viologen (MV)/4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (4-HO-TEMPO) redox flow battery. This paper aims to solve the insufficient adhesion of the dip-coating method by simple, effective and low-cost means and provides a possibility for large-scale production and application; the new modification method increases the reaction area and performance of the electrode, resulting in high current density and improved battery performance, and in the current density of 60 mA cm⁻², the battery provides 97.39% theoretical capacity, which has practical significance for battery configurations.

Graphene felt electrodes modified with reduced graphene oxide (rGO) can greatly improve the performance of MV/ 4-HO-TEMPO flow battery.