乙型肝炎疫苗与婴儿期常用疫苗同时接种的免疫应答与反应观察:Ⅱ.乙型肝炎疫苗与乙型脑炎、麻疹疫苗同时接种的免疫应答与反应观察

本文对HBV与JEV、MV同时接种的免疫应答性和反应作了研究。对0~9月龄215名婴儿随机分为Ⅰ组单独接种HBV,Ⅱ组分别接种JEV、MV,Ⅲ组HBV分别与MV、JEV同时接种,三针间隔为0、1、5个月。均作免前和免后的血清抗体测定,同时接种组的抗-HBs,JEV抗体的阳转率,GMT与单独接种无显著性差异,JEV抗体低于常规接种,MV的免疫应答性Ⅲ组低于Ⅱ组,有显著性差异。未见异常反应,一般反应均很轻微,二组间无差别,说明MV不宜与HBV同时接种,JEV不能用HBV免疫程序同时接种。     

针刺对实验性脊髓损伤前角运动神经元酶学的影响

目的：探讨针刺对大鼠脊髓损伤前角运动神经元酶学的影响。方法：采用酶组织化学染色方法定性分析针刺治疗前后脊髓前角运动神经元乙酰胆碱酯酶（ＡＣＨＥ），琥珀酸脱氢酶（ＳＤＨ）、酸性磷酸酶（ＡＣＰ）的含量变化；同时，运用全自动义系统进行定量分析。结果：脊髓损伤后ＡＣＨＥ和ＳＤＨ含量减少；ＡＣＰ含量增加。而针刺组针刺后均较对照组同时段点ＡＣＨＥ、ＳＤＨ酶含量高；ＡＣＰ酶含量低，结论：针刺能调节脊髓损伤后前角     

Enhanced Stability of Calcium Sulfate Scaffolds with 45S5 Bioglass for Bone Repair

Calcium sulfate (CaSO₄), as a promising tissue repair material, has been applied widely due to its outstanding bioabsorbability and osteoconduction. However, fast disintegration, insufficient mechanical strength and poor bioactivity have limited its further application. In the study, CaSO₄ scaffolds fabricated by using selective laser sintering were improved by adding 45S5 bioglass. The 45S5 bioglass enhanced stability significantly due to the bond effect of glassy phase between the CaSO₄ grains. After immersing for four days in simulated body fluid (SBF), the specimens with 45S5 bioglass could still retain its original shape compared as opposed to specimens without 45S5 bioglass who experienced disintegration. Meanwhile, its compressive strength and fracture toughness increased by 80% and 37%, respectively. Furthermore, the apatite layer was formed on the CaSO₄ scaffolds with 45S5 bioglass in SBF, indicating good bioactivity of the scaffolds. In addition, the scaffolds showed good ability to support the osteoblast-like cell adhesion and proliferation.     

康莱特联合化疗对NSCLC患者生活质量改善的临床研究

目的　探讨康莱特注射液对联合化疗与单纯化疗改善晚期非小细胞肺癌 (NSCL C)患者的生活质量临床意义。方法　采取随机对照分组 ,治疗组 30例为康莱特联合化疗 ;对照组 30例为单纯化疗。结果　治疗组有效率为 36 .7% ,对照组为 2 3.3% ,有显著性差异 (P<0 .0 5 ) ,在控制疼痛、体重及 karnofsky评分等方面治疗组均优于对照组 ,差异有显著性。结论　康莱特注射液联合化疗可提高化疗疗效、改善患者生活质量     

Pklr基因敲除小鼠的构建及其糖脂代谢研究

目的 丙酮酸激酶(pyruvate kinase,PK)作为糖酵解途径的关键限速酶之一,主要在肝脏和红细胞中表达,在糖脂代谢过程中发挥着重要作用.对于PK的研究目前主要集中在细胞实验与临床观察水平,动物模型的缺乏限制了对其进一步研究.因此该研究目的在于建立Pklr基因缺陷小鼠模型,并探索该酶在机体中与糖脂代谢的关系.方法 本研究利用CRISPR/Cas9系统,设计引物sgRNA1与sgRNA2,分别靶向LPK外显子1和共用外显子3.通过测序、实时定量PCR、Western印迹及PK活性测定试剂盒检测小鼠Pklr基因在转录、蛋白质和酶活性等不同层面上的变化,之后通过正常与高脂饮食,检测小鼠体重、白色脂肪及脾脏变化验证PK与糖脂代谢的关系.结果 经过检测发现,小鼠Pklr基因双位点发生基因编辑,与野生型小鼠相比,转录水平下降至24％,LPK蛋白几乎不表达,酶活性下降至33％,在基因、转录、蛋白质和酶活性不同层面上证明成功获得了Pklr基因缺陷小鼠模型,并且发现该基因突变纯合子小鼠在高脂饮食条件下显示出体重下降和白色脂肪积累(P值分别为0.0040、0.0200),同时两种饮食条件下脾脏重量都显著上升(P =0.0003).结论 利用CRISPR/Cas9系统成功构建了Pklr基因缺陷小鼠,并且该基因缺陷会导致异常的糖脂代谢.Pklr基因敲除小鼠模型为进一步探索PK在机体内调控机制与糖脂代谢的关系提供了平台.     

接种流感病毒疫苗后触发系统性红斑狼疮一例并文献复习

目的：探讨外界环境因素在系统性红斑狼疮（SLE）发病机制中的作用。方法：报道1例男性14岁患者在接种流感病毒疫苗后触发了SLE疾病的发生，并对患者的一级亲属的血清进行了免疫学检测。结果：患儿父亲血清中有高滴度的抗核抗体。结论：外界环境因素如流感病毒疫苗免疫注射可能触发有内在遗传基因背景的个体发生SLE。对有SLE易感倾向的个体及SLE患者应减少非计划内免疫接种的次数。     

microRNA expression profile and differentially-expressed genes in prolactinomas following bromocriptine treatment

Little is known about the function of microRNAs in prolactinomas treated with bromocriptine. The aim of the study was to explore the microRNAs associated with bromocriptine-treated prolactinomas. Six prolactinoma samples were selected according to whether they received bromocriptine treatment or not before microsurgery, and microRNA expression profiles of bromocriptine-treated and untreated prolactinomas were screened by the miRCURY LNA Array. The differentially expressed microRNAs in microarrays were further validated by stem-loop real-time PCR and subjected to gene ontology analysis and KEGG pathway analysis. In addition, related genes of microRNAs were analyzed by qRT-PCR in 15 prolactinoma samples. The initial analysis by microarrays generated a list of 80 upregulated microRNAs and 71 downregulated microRNAs in treated prolactinomas compared to untreated prolactinomas. miR-206, miR-516b and miR-550 were confirmed to be significantly upregulated, while miR-671-5p was confirmed to be significantly downregulated in treated prolactinomas by qRT-PCR. microRNA-mRNA network analysis integrating GO and KEGG pathway annotation displayed some critical factors. Platelet-derived growth factor α polypeptide (PDGFA) and bone morphogenetic protein 4 (BMP4), were verified to be differentially expressed between the two groups. PDGFA was significantly upregulated in treated prolactinomas, while BMP4 was significantly downregulated in treated prolactinomas. Our study reveals differential expression of microRNAs in prolactinoma after pharmacotherapy. Specific microRNAs may be involved in the inhibition or promotion of prolactinoma tumor growth impacted by bromocriptine pharmacotherapy. PDGFA and BMP4 may be involved in the pharmacotherapy mechanism of prolactinoma.     

干扰素对老年女性丙型肝炎患者甲状腺功能的影响

目的:探讨老年女性慢性丙型肝炎患者干扰素治疗前后血清甲状腺激素水平的变化。方法:检测13例老年女性慢性丙型肝炎患者在干扰素治疗不同时间甲状腺激素水平,并与26例中青年丙型肝炎患者、15例老年男性患者和20例同期正常体检者作对照研究。结果:在干扰素治疗12周时,老年女性组与各对照组比较,TSH升高,T3、T4、FT3、FT4明显低于治疗前水平(P<0.05)。结论:干扰素治疗可导致甲状腺激素代谢紊乱,在老年女性患者更容易出现甲状腺功能减退。     

实验性大鼠恶性胸膜间皮瘤的CT表现与病理对照研究

目的 探讨大鼠恶性胸膜间皮瘤(Malignant pleural mesothelioma,MPM)CT表现.材料与方法对66只Wistar大鼠MPM模型行胸部CT平扫,并尸解及病检.结果 胸膜增厚形态与组织病理类型、分化程度无关.CT显示局限片状增厚18侧、结节形增厚48侧、弥漫环形增厚47侧,19侧CT未显示胸膜增厚.CT胸水发现率为39.13%,多见于上皮型和混合型.镜下MPM向周围组织浸润45例,占68.18%,CT仅显示33.33%.结论 平扫CT不能提示MPM组织类型和分化程度;胸膜增厚的类型与大体病理学改变有一定符合性,但CT不能显示小于3 mm结节和薄的斑片状病灶;CT对胸水显示率低.