Polyetheretherketone/poly (glycolic acid) blend scaffolds with biodegradable properties

Polyetheretherketone (PEEK) is widely applied in tissue engineering due to its good biocompatibility and mechanical properties. However, the slow degradation rate limits its further application. In this study, PEEK blended with plyglycolicacid (PGA) was used to fabricate porous scaffolds via selective laser sintering. The results demonstrated that the blend scaffolds could gradually degrade, and the degradation rate was able to regulate by tailoring the PGA content. Moreover, the scaffolds maintained good biocompatibility and suitable mechanical properties. These were explained as follows: PGA on the surface layer of the scaffolds might degrade first owing to its exposure to the ambient medium. The degraded PGA left much space, which could promote cell attachment and proliferation. Meanwhile, the slow degradation of PEEK was beneficial to sustaining the scaffolds’ strength and stable structure.     

性咨询治疗功能性不射精症的临床分析

自1988年5月至1996年6月,我站共治疗功能性不射精症87例。以性心理咨询和性指导为主,治疗效果明显,现报告如下。一、一般资料1.年龄 20—25岁45例,26—30岁25例,31—35岁12例,>35岁5例。2.职业农民72例,工人12例,干部3例。3.文化程度文盲20例,小学51例,中学12例,大专以上4例。     

应用QCT探索骨质疏松症诊断及分级诊断标准

目的　应用QCT检测健康成年人腰椎骨密度 ,探索骨质疏松症的诊断及分级诊断标准。方法　采用日本东芝制造 60 0HQ的CT(单能 ) ,对 12个年龄段 (5年为 1段 ) 5 14名 (男 2 0 6人 ,女3 0 8人 )健康志愿者 (除外患有影响骨代谢疾病及严重腰椎疾病者 ) ,进行L3松质骨BMD检测。结果①峰值骨密度位于 3 0岁年龄段 ,男性 (2 2 7 8± 2 7 0 )mg/cm3;女性 (2 40 9± 2 9 0 )mg/cm3。②峰值骨量过后BMD随增龄而逐渐降低 (P <0 0 1) ,至 45岁年龄段时 ,男女均进入骨量减少期 :女性 5 0岁、男性5 5岁年龄段时BMD均值进入骨质疏松期。③骨质疏松诊断标准有二 ,第一 ,BMD测定值比同性别峰值BMD均值降低 2 5SD以上。第二 ,BMD测定值比同性别峰值BMD减少 3 0 %以上可做骨质疏松诊断标准。④首次提出骨质疏松分 4级 (Ⅰ、Ⅱ、Ⅲ、Ⅳ级 )诊断标准及诊断量化表。结论　①QCT诊断骨质疏松具有较高的敏感性、准确性及可重复性。②骨质疏松分级诊断标准 ,给临床诊断、疗效观察及科研提供具体客观指标。③诊断量化表能帮助临床医师直接、准确、快捷的做出骨质疏松症的分级诊断并易于推广应用     

Influence of leptin on luteinizing hormone and follicle stimulating hormone secreted from cultured rat anterior pituitary cells

BACKGROUND: Leptin may regulate reproductive function via release of hypothalamic neuropeptide Y. However, it is unknown whether this regulatory effect is limited to the hypothalamus. OBJECTIVE: To detect the effect of different dosages of leptin on luteinizing hormone (LH) and follicle stimulating hormone (FSH) secretion from in vitro cultured rat anterior pituitary cells. DESIGN: Contrast study based on cells. SETTING: This study was performed in the Basic Institute of Chengde Medical College, Chengde City, Hebei Province, China from March to June 2007. MATERIALS: Eighteen female Wistar rats of three months of age, weighing 200-220 g, and of clean grade were used. Leptin was provided by Peprotech Company, DMEM culture medium by Invitrogen Company, and the radioimmunological kit by Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. METHODS: Three glandular organs were regarded as one group for culture of anterior pituitary cells. In the control group, saline was added to the culture medium instead of leptin. In the leptin group, leptin was prepared into different concentrations of 1×10-12, 1×10-11, 1×10-9, 1×10-7, and 1×10-6 mol/L for stimulation of cultured cells. The culture supernatant was obtained at three hours after additional of saline/leptin. MAIN OUTCOME MEASURES: Contents of LH and FSH were detected by radioimmunology. RESULTS: Following leptin stimulation, LH release increased with increasing concentrations of leptin up to 1×10-9 mol/L, where LH release peaked. LH release then progressively decreased with increasing leptin concentrations (P<0.01). LH release in the leptin (1×10-12, 1×10-11, 1×10-7, and 1×10-6 mol/L) groups was significantly higher than that in the control group (P<0.01). FSH content in the leptin (1×10-11, 1×10-9, and 1×10-7 mol/L) groups was significantly higher than that in the control group (P<0.01). CONCLUSION: Leptin can directly affect pituitary tissue to promote the secretion of LH and FSH in a dose-dependent manner.     

MRP、P-gp在非小细胞肺癌中的表达及临床意义

目的　观察多药耐药相关蛋白 (MRP)、P-糖蛋白 (P- gp)在肺癌组织中的表达情况及与临床病理的关系 ,以及 MRP与 P- gp之间的相关性 ,探讨 MRP、P- gp与肺癌患者预后的关系。方法　应用 S- P免疫组化法检测非小细胞肺癌 MRP、P- gp的表达情况。结果　 4 2例非小细胞肺癌中 MRP阳性率为 4 5 .2 % ,腺癌 MRP的表达明显高于鳞癌 (P<0 .0 5 )。 P- gp阳性表达率为 4 0 .4 % ,P- gp的表达与组织类型、细胞分化程度、TNM临床分期无关 (P>0 .0 5 )。 MRP、P- gp阴性患者术后 1、3年无病生存期 (DFS)明显高于 MRP、P- gp阳性患者 (P<0 .0 1)。肺癌组织中 P- gp与 MRP的表达具有明显相关性 (P<0 .0 5 )。结论　 MRP、P- gp可作为临床判断化疗疗效的参考指标及预测肺癌患者预后的指标     

益肾降浊汤对脑缺血再灌注大鼠海马EAA含量的影响

目的 :观察益肾降浊汤对脑缺血再灌注大鼠海马组织EAA含量的影响。方法 :用氨基酸自动分析仪测定游离氨基酸浓度。结果 :第 1天模型大鼠海马Glu、Asp含量分别是 ( 8.2 6± 2 .13 ) μmol/g、( 2 .10± 0 .93 ) μmol/g ,第 7天含量分别是 ( 7.2 7± 2 .2 0 ) μmol/g、( 1.87± 2 .0 1) μmol/g ,第 15天含量分别是 ( 9.3 6± 2 .2 4) μmol/g、( 1.42± 1.2 1) μmol/g ,均较正常对照组显著降低 (P <0 .0 5～ 0 .0 1)。而中药组大鼠第 1天脑海马Glu、Asp含量分别是 ( 9.90± 3 .2 0 )μmol/g、( 2 .95± 1.73 ) μmol/g ,第 7天含量分别是 ( 10 .49± 2 .3 4)μmol/g、( 3 .0 3± 1.0 9) μmol/g ,第15天含量分别是 ( 11.0 5± 2 .5 4)μmol/g、( 3 .0 5± 1.41) μmol/g ,均高于同期模型组 (P <0 .0 5 )。结论 :益肾降浊汤可提高缺血再灌注后脑组织细胞内的EAA含量 ,调节突出间隙EAA浓度 ,对缺血神经元有保护作用。     

QT Measurement: A Comparison of Three Simple Methods

Background: QT interval and QT dispersion are useful noninvasive measurements in clinical cardiology and can be measured by several methods. The comparative variability of these methods, however, is not well defined. Methods: We evaluated the intra- and interobserver variability of three simple methods of QT measurement: (1) ruler method: use of a 0.5-mm scale precision ruler to measure QT with end of T wave determined by extrapolating its slope to baseline; (2) caliper method: use of a standard electrocardiogram (ECG) caliper and the standard ECG paper scale with QT determined by visual inspection; (3) computer method: use of a digitized computer software program with QT determined by cursor set manually by the user. QT intervals from 11 patients (total 44 ECG leads) in sinus rhythm without conduction defect were measured by five blinded, trained observers at two time points (a week apart) in a crossover manner. Results: The mean difference in intraobserver measurements were 6 ± 2, 12 ± 12, and 27 ± 2 ms by the ruler, caliper, and computer methods, respectively (P > 0.01, ruler vs caliper or computer). The mean differences in interobserver measurements were 13 ± 3, 16 ± 4, and 29 ± 3 ms for the same methods, respectively (P > 0.01, ruler vs caliper, computer). Enlargement of the ECG to 200% did not reduce the measurement variability. Conclusion: The ruler method as described yielded the lowest variability in QT measurement.     

良、恶性胸水表达癌胚抗原信使核糖核酸--癌胚抗原系统差别的研究

目的分析癌胚抗原信使核糖核酸(CEAmRNA)-癌胚抗原(CEA)系统在良、恶性胸水中表达的差别,探讨应用CEAmRNA和CEA指标诊断恶性胸水的价值.方法采集胸水为检测标本,其中恶性胸水92例、良性胸水54例.应用反转录-套式-聚合酶链反应(RT-NP-PCR)检测CEAmRNA,应用磁性抗体分离酶联免疫测定(MAIA)检测CEA.结果不同胸水表达CEAmRNA和CEA的阳性结果分别为良性5.6%(3/54)和7.4%(4/54)、恶性80.4%(74/92)和54.3%(50/92),两者差别具有统计学意义(P＜0.001).指标诊断恶性胸水的特异性和敏感性分别为CEAmRNA 94.4%和80.4%,CEA分别为92.6%和54.3%,CEAmRNA的敏感性明显高于CEA(P＜0.01).结论CEAmRNA-CEA系统在良、恶性胸水中的表达的差别具有统计学意义,可以用作诊断与鉴别诊断恶性胸水的指标.其中,CEAmRNA在恶性胸水中的表达率和敏感性比CEA更高,有望成为一个新的、有实用价值的肿瘤标志物.     

心肌与血管再生的中西医研究进展

动物实验和初步人体临床试验研究表明,经冠状动脉灌注或心肌直接注射自体干细胞或祖细胞能减轻心肌的疤痕形成和纤维化,增加心肌新生血管的形成达到增加心肌血流量.一些中药也可通过刺激心肌缺血区各种血管生成因子和旁分泌达到促进血管生长的目标.     

恶性抗磷脂综合征7例临床分析并文献复习

目的：探讨恶性抗磷脂综合征（CAPS）的临床特点及预后，以期提高临床医生对该病的认识及诊治水平。方法：回顾性分析我院风湿科收治的7例恶性抗磷脂综合征患者临床表现、实验室检查及预后等资料并复习相关文献。结果：7例CAPS患者中，1例为原发APS，6例为SLE继发APS。静脉血栓发生5例次，主要是下肢深静脉血栓、眼中央静脉栓塞；动脉血栓13例次，以脑梗死为主（6例次）。6例抗心磷脂抗体阳性，3例抗β2-GPⅠ抗体阳性，3例狼疮抗凝物阳性。5例患者在1周内相继出现心、肺、肾等多器官功能衰竭而导致死亡。结论：CAPS患者短时间内发生大量微血栓形成，可导致多器官功能衰竭。本病的预后差，及早诊断、及时治疗是改善患者预后的关键。