安徽省人类免疫缺陷病毒1型流行毒株基因序列变化和亚型特征分析

我国人类免疫缺陷病毒1型(human immunodeficiency virus 1,HIV-1)流行毒株亚型复杂,自1996年以来,已报道A,B,B',C,D,F,亚型CRF01_AE,CRF02AG和CRF_BC重组毒株等亚型在我国同时流行[1-2].     

中国南方四省区流行的HIV-1 CRF01_AE病毒株基因特征研究

目的 分析中周南方四省区HIV-1感染者中流行CRF01 AE病毒株的遗传特征.方法 从广东、广西、江西和湖南省(自治区)2006年新报告HIV-1感染者的血浆样本中提取病毒RNA,用反转录/巢式PCR方法扩增gag和env基因片段,对获得的CRF01 AE病毒株核酸序列进行系统进化分析,并通过计算基因距离和Entropy核苷酸多态性差异方法分析毒株的遗传特征.结果 从210例CRF01_AE病毒株感染者中,发现四省区流行的CRF01 AE病毒株存在2个主要的流行簇.流行簇Ⅰ共有123例样本,未发现与之直接相关的国际参考毒株.流行簇Ⅱ共有57例样本,与越南CRF01 AE病毒株关系密切,且存在不同时间样本的混杂.gag和env基因遗传距离分析结果表明,流行簇Ⅰ内基因遗传多样性均明显小于流行簇Ⅱ;核苷酸多态性分析结果显示,在gag基因片段42个位点核苷酸组成具有显著差异,env基因片段40个位点核苷酸组成存在显著差异;流行簇Ⅰ相对于流行簇Ⅱ多态性减少的位点上有61个,多态性增加的位点有21个.结论 在中国南方四省区流行的CRF01 AE病毒株中首次观察到2个独立的流行簇.流行簇Ⅰ病毒株为该地区最主要的CRF01 AE病毒株,其流行时间相对较短,在人群中所占比例较多,可能是病毒在流行过程中形成的具有传播优势的病毒株.流行簇Ⅱ病毒与来自于越南的CRF01 AE病毒株有较高同源性,且存在与越南病毒株间的多次传播.     

河北省2007年HIV-1流行毒株基因序列测定及亚型分析

HIV具有基因易变性特点,为探讨河北省HIV-1感染人群传播特点,监测不同人群中亚型毒株的分布和基因变异规律,开展了此项研究.     

中国南方四省区流行的HIV-1 CRF01_AE病毒株基因特征研究

Objective To analyze the genetic characteristics of HIV-1 CRF01_AE strains prevailing in. the four provinces of southern China. Methods Plasma samples were collected from the newly diagnosed HIV-1 individuals reported in 2006 in Guangdong, Guangxi, Jiangxi and Hunan province. The gag and env gene fragments were amplified from RNA template extracted from plasma using RT and nested PCR methods. CRF01_AE sequences were analyzed by phylogcnetie methods and characterized by calculating the genetic distance and Entropy analysis. Results Two main epidemic clusters were found to exist in the CRF01 AE strains from 210 HIV-1 CRF01 AE infected individuals collected in the 4 provinces, southern China. It was found that no international reference strain was closely correlated with cluster Ⅰ , which including 123 samples. The strains in cluster Ⅱ, consisting 57 cases of samples, were closely related with the strains identified in Vietnam. Genetic distance analysis of gag and env genes showed that the diversity of cluster Ⅰ was obviously less than that of cluster Ⅱ. Data on nucleotide polymorphism showed that nucleotides compositions of 42 sites in gag and 40 sites in env wer esignificantly different between the two clusters. When compared with cluster Ⅱ , the polymorphism decreased at 61 nucleotide sites but increased at 21 sites in cluster Ⅰ. Conclusion This was the first report describing that two main epidemic clusters were existed in CRF01_AE strains prevailing in the 4 provinces, Southern China. The vires in cluster Ⅰ was the dominant strain in this region, with shorter period of circulation and higher proportion seen in the HIV-infected population, which might belong to CRF01_AE strain with certain features facilitating the spread of the virus. The virus in cluster Ⅱ was highly homology with the CRF01_AE strains from Vietnam, and seemed to have had several events of epidemics in populations in border regions of China and Vietnam.     

小剂量伊曲康唑加茵陈蒿治疗甲癣45例

经灰黄霉素与茵陈蒿联用可以减少灰黄霉素用量 ,降低副反应的提示 ,我们用小剂量伊曲康唑与茵陈蒿联用冲击治疗甲癣 ,收到较佳效果 ,现报道于下。临床资料　随机选取 2 0 0 0年度门诊就诊的 90例甲癣患者 ,诊断符合《实用皮肤科学》(刘辅仁主编 .第 2版 .北京 :人民卫生出版社 ,1999∶2 0 2— 2 0 3 )关于甲癣的诊断要点。排除孕产、哺乳期及 18岁以下患者。常规检查肝、肾功能 ,血常规及血糖正常 ,HBsAg阴性。其病甲均增厚变暗、无光泽、易碎、真菌显微镜镜检均见真菌菌丝。将 90例患者分为两组。其中A组男 17例 ,女 2 8例 ;年龄 19～ 69…     

类风湿Ⅱ号口服液治疗风寒湿痹的研究

类风湿性关节炎、风湿性关节炎及风湿性关节痛是一类临床治疗较为棘手的风湿痹证。我们在多年临床的基础上，研制出类风湿Ⅰ号、Ⅱ号方分别其不同证型而治之，取得了较为满意的疗效。现将１９９０年８月～１９９３年８月，我们运用类风湿Ⅱ号方治疗风寒湿痹２４２例的观察...     

中国南方四省区流行的HIV-1 CRF01_AE病毒株基因特征研究

Objective To analyze the genetic characteristics of HIV-1 CRF01_AE strains prevailing in. the four provinces of southern China. Methods Plasma samples were collected from the newly diagnosed HIV-1 individuals reported in 2006 in Guangdong, Guangxi, Jiangxi and Hunan province. The gag and env gene fragments were amplified from RNA template extracted from plasma using RT and nested PCR methods. CRF01_AE sequences were analyzed by phylogcnetie methods and characterized by calculating the genetic distance and Entropy analysis. Results Two main epidemic clusters were found to exist in the CRF01 AE strains from 210 HIV-1 CRF01 AE infected individuals collected in the 4 provinces, southern China. It was found that no international reference strain was closely correlated with cluster Ⅰ , which including 123 samples. The strains in cluster Ⅱ, consisting 57 cases of samples, were closely related with the strains identified in Vietnam. Genetic distance analysis of gag and env genes showed that the diversity of cluster Ⅰ was obviously less than that of cluster Ⅱ. Data on nucleotide polymorphism showed that nucleotides compositions of 42 sites in gag and 40 sites in env wer esignificantly different between the two clusters. When compared with cluster Ⅱ , the polymorphism decreased at 61 nucleotide sites but increased at 21 sites in cluster Ⅰ. Conclusion This was the first report describing that two main epidemic clusters were existed in CRF01_AE strains prevailing in the 4 provinces, Southern China. The vires in cluster Ⅰ was the dominant strain in this region, with shorter period of circulation and higher proportion seen in the HIV-infected population, which might belong to CRF01_AE strain with certain features facilitating the spread of the virus. The virus in cluster Ⅱ was highly homology with the CRF01_AE strains from Vietnam, and seemed to have had several events of epidemics in populations in border regions of China and Vietnam.     

隐翅虫皮炎71例误诊分析

正隐翅虫皮炎是春、夏、秋季常见皮肤病,如果临床经验不足、或皮损不典型也常被误诊为其他皮肤病,现将我科收集整理的被误诊的71例病例进行归纳总结、分析如下。1临床资料1.1一般资料71例误诊的隐翅虫皮炎病例均为外院误诊,来我科就诊后确诊的2015年至2016年门诊病例,诊断均符合冉玉平~([2])隐翅虫皮炎临床皮疹。其中男53例,女18例,(5～10)岁8例,     

皮损内注射低分子肝素钙治疗睑黄瘤疗效评价

普通肝素钠局部注射治疗睑黄瘤已应用多年,且有效[1-4].使用低分子肝素钙治疗睑黄瘤很少报道.我们采用皮损内注射低分子肝素钙治疗睑黄瘤,并与以往液氮冷冻治疗作回顾性对照,对比其疗效、不良反应及复发情况.