毒力虫霉EB-82灭蚜菌发酵产物的有效成分研究Ⅱ.化合物EB-82B的分离和结构鉴定

从毒力虫霉ＥＢ－８２灭蚜菌的发酵产物中提取分离得到的一单体化合物ＥＢ－８２Ｂ，经过ＩＲ、ＥＩ－ＭＳ、ＵＶ、１Ｈ－ＮＭＲ、１３Ｃ－ＮＭＲ、ＤＥＰＴ等波谱分析鉴定为二十六烷酸（ｈｅｘａｃｏｓａｎｏｉｃａｃｉｄ）。田间实验表明该发酵产物有促生长作用，二十六烷酸的存在可能是该促生长作用的重要因素之一     

白茅根化学成分的研究

从白茅（ＩｍｐｅｒａｔａｃｙｌｉｎｄｒｉｃａＢｅａｕｖ．ｖａｒｍａｊｏｒ（Ｎｅｅｓ）ＣＥＨｕｂｂ．）的根茎中分离得到１６个化合物，根据理化性质及波谱分析鉴定了其中５个化合物，分别为：α－联苯双酯（Ｄｉｍｅｔｈｌ４，４－ｄｉｍｅｔｈｏｘｙ－５，６，５′，６′－ｄｉｍｅｔｈｙｌｅｎｅｄｉｘｙｂｉｐｈｅｎｙ１－２，２′－ｄｉｃａｒｂｏｘｙｌａｔｅ，Ｉ），对羟基桂皮酸（ｐＣｏｕｍａｒｉｃ，ａｃｉｄ，Ⅱ）     

ACTAPHARMACEUTICASINICA，Vol30，No．1_12kE

ＡＣＴＡＰＨＡＲＭＡＣＥＵＴＩＣＡＳＩＮＩＣＡ，Ｖｏｌ３０，Ｎｏ．１～１２ＫＥＹＷＯＲＤＳＩＮＤＥＸＡＡ２７８０／ＡＤＭ（４）：２６２Ａｃｅｔａｍｉｄｅｃｈｌｏｒｏｐｈｅｎｏｘｙ（７）：５６０Ａｃｅｔｉｃａｃｉｄ，２－ｃａｒｂｏｍｅｔｈｏｘｙ－（２）...     

Triterpenoids from the Fruiting Body of Ganoderma lucidum

自赤芝［Ｇａｎｏｄｅｒｍａｌｕｃｉｄｕｍ（Ｆｒ．）Ｋａｒｓｔ］的二氯甲烷溶解部分分离得到一个新的三萜内酯化合物，命名为灵芝内酯（ｇａｎｏｌａｃｔｏｎｅ）。根据光谱（ＵＶ，ＩＲ，１ＨＮＭＲ，１３ＣＮＭＲ，ＭＳ和２ＤＮＭＲ）解析，确定其结构为１式。同时还从赤芝子实体中分到三个已知化合物，即灵芝醇Ａ（ｇａｎｏｄｅｒｉｏｌＡ，２），灵芝醇Ｂ（ｇａｎｏｄｅｒｉｏｌＢ，３）和灵芝三醇（ｇａｎｏｄｅｒｍａｔｒｉｏｌ４）。     

中间锦鸡儿新三萜甙化合物的结构研究

从中间锦鸡儿（ＣａｒａｇａｎａｉｎｔｅｒｍｅｄｉａＫ．）根部分离得到１１种化合物成分，分别为３棕榈酰β谷甾醇（３ｐａｌｍｉｔｏｙｌβｓｉｔｏｓｔｅｒｏｌ）；晁模酸（ｃｈａｕｌｍｏｇｒｉｃａｃｉｄ）；晁模酸乙酯（ｅｔｈｙｌｃｈａｕｌｍｏｏｇｒａｔｅ）；羽扇醇（ｌｕｐｅｏｌ）；胡罗卜甾醇（ｄａｕｃｏｓｔｅｒｏｌ）；三十一烷（ｈｅｎｔｒｉａｃｏｎｔａｎｅ）；α棕榈油酰甘油（αｐａｌｍｉｔｏｌｅｏｙｌｇｌｙｃｅｒｏｌ）；α棕榈酰甘油酯（αｐａｌｍｉｔｏｙｌｓｎｇｌｙｃｅｒｏｌ）；β谷甾醇（βｓｉｔｏｓｔｅｒｏｌ）；锦鸡儿甙１（ｃａｒａｇａｎｏｓｉｄｅＡ）和锦鸡儿甙２（ｃａｒａｇａｎｏｓｉｄｅＢ）。其中锦鸡儿甙１和锦鸡儿甙２为新天然产物。应用现代波谱技术（ＩＲ，ＵＶ，ＭＳ，２ＤＮＭＲ等）和化学降解方法确定了它们的化学结构     

女贞子化学成分的研究(Ⅱ)

继前报，作者又从女贞子（ＬｉｇｕｓｌｒｕｍＬｕｃｉｄｕｍＡｉｌ．）乙醇提取物的石油醚萃取部分和乙酸乙酯萃取部分分离鉴定了４个化合物．化合物Ⅵ为１９α－羟基－３－乙酰乌索酸（１９α－ｈｙｄｒｏｘｙ－３－ａｃｅｔｙｌ－ｕｒｓｏｌｉｃａｃｉｄ），化合物Ⅸ为对羟基苯乙醇－β－Ｄ－葡萄糖甙（ｐ－ｈｙｄｒｏｘｙｐｈｅｎｅｔｈｙｌ－β－Ｄ－ｇｌｕｃｏｓｉｄｅ），化合物Ｘ为芹菜素－７－Ｏ－β－Ｄ－葡萄糖甙（ｃｏｓｍｏｓｓｉｎ），化合物Ⅺ为甘露醇（ｍａｎｎｉｔｏｌ）．其中化合物Ⅵ为一新天然产物．     

莶脂溶性成分的研究

从莶（ＳｉｅｇｅｓｂｅｃｋｉａｏｒｉｅｎｔａｌｉｓＬ）的地上部分，分离出八个化合物，其中Ｉ和Ｉ根据理化性质和光谱数据确定其结构为ｅｎｔ１７ａｃｅｔｏｘｙ１８ｉｓｏｂｕｔｙｒｙｌｏｘｙ１６（α）ｋａｕｒａｎ１９ｏｉｃａｃｉｄ（Ｉ）和ｅｎｔ１７ｅｔｈｏｘｙ１６（α）ｋａｕｒａｎ１９ｏｉｃａｃｉｄ（ＩＩ），均为新化合物，分别被命名为莶酯酸（ｓｉｅｇｅｓｅｓｔｅｒｉｃａｃｉｄ，Ｉ）和莶醚酸（ｓｉｅｇｅｓｅｔｈｅｒｉｃａｃｉｄ，Ｉ）。其余化合物分别鉴定为腺梗莶萜醇酸（ｅｎｔ１６β，１７ｄｉｈｙｄｒｏｘｙｋａｕｒａｎ１９ｏｉｃａｃｉｄ，ＩＩ），奇任醇（ｋｉｒｅｎｏｌ，ＩＶ，β谷甾醇葡萄糖甙（βｓｉｔｏｓｔｅｒｏｌｇｌｕｃｏｓｉｄｅ，Ｖ），二十一醇（ｈｅｎｅｉｃｏｓａｎｏｌ，ＶＩ），花生酸甲酯（ｍｅｔｈｙｌａｒａｃｈｉｄａｔｅ，ＶＩＩ）和β谷甾醇（βｓｉｔｏｓｔｅｒｏｌ，ＶＩＩ）。除奇任醇和β谷甾醇外，均为首次从该植物中分得。     

茎点霉299产生的免疫抑制剂 Ⅱ.SIPI—299—I,299—Y和2

对真菌茎点霉２９９的进一步研究,又从中分离得到３个活性化合物２９９－Ｉ、２９９－Ｙ和２９９－ＡＤ。经波谱分析（ＵＶ、ＥＩ－ＭＳ、＾１Ｈ－ＮＭＲ、＾１３Ｃ－ＮＭＲ－ＤＥＰＴ、ＩＲ）,证实它们分别与ｒｏｒｉｄｉｎ Ａ、Ｄ和Ｈ相同,也属于大环内酯ｔｒｉｃｂｏｔｈｅｃｅｎｅｓ类化合物。３个化合物均有强的免疫抑制性。     

女贞子化学成分的研究（Ⅱ）1）

继前报，作者又从女贞子（Ｌｉｇｕｓｔｒｕｍ Ｌｕｃｉｄｕｍ Ａｉｔ。）乙醇提取物的石油醚萃取部分和乙酸乙酯萃取部分分离鉴定了４个化合物。化合物Ⅵ为１９ａ－羟基－３－乙酰乌索酸（１９ａ－ｈｙｄｒｏｘｙ－３－ａｃｅｔｙｌ－ｕｒｓｏｌｉｃ ａｃｉｄ），化合物Ⅸ为对羟基苯乙醇－β－Ｄ－葡萄糖甙（ｐ－ｈｙｄｒｏｘｙｐｈｅｎｅｔｈｙｌ－β－Ｄ－ｇｌｕｃｏｓｉｄｅ），化合物Ｘ为芹菜素－７－Ｏ－β－Ｄ－葡萄糖甙（     

麻栎叶的化学成分研究

从麻栎（ＱｕｅｒｃｕｓａｃｕｔｉｓｉｍａＣａｒｕｔｈ．）的叶中分离得到５个化合物,根据理化数据和ＩＲ,１Ｈ ＮＭＲ,１３Ｃ ＮＭＲ,ＤＥＰＴ谱鉴定为表木栓醇（１）,β 粘霉烯醇（２）,羽扇豆醇（３）,木栓醇（４）和β 谷甾醇（５）,表木栓醇和木栓醇为本种植物首次分离     

赤芝孢子粉三萜化学成分研究

自赤芝(Ganoderma lucidum Karst)孢子粉酸性部分分离得到一个新的四环三萜化合物,命名为ganosporeric acid A(1),根据光谱(IR,¹HNMR,¹³CNMR和MS)分析确定其结构为Ⅰ所示。同时还分到四个已知化合物:ganoderic acid B(Ⅱ),ganodefic acid C(Ⅲ),ganoderic acidE(Ⅳ),和ganodermanontriol(Ⅴ)。这些化合物均为首次从赤芝孢子粉中得到。     

Constituents from the fruiting bodies ofGanoderma applanatum and their aldose reductase inhibitory activity

Eight compounds were isolated from the fruiting bodies of Ganoderma applanatum, and were identified as 2-methoxyfatty acids (1), 5-dihydroergosterol (2), ergosterol peroxide (3) 3beta,7beta, 20,23xi-tetrahydroxy-11,15-dioxolanosta-8-en-26-oic acid (4), 7beta,20,23xi-trihydroxy-3,11,15-trioxolanosta-8-en-26-oic acid (5), cerevisterol (6), 7beta,23xi-dihydroxy-3,11,15-trioxolanosta-8,20E (22)-dien-26-oic acid (7), and 7beta-hydroxy-3,11,15,23-tetraoxolanosta-8,20E(22)-dien-26-oic acid methyl ester (8) by spectral analysis. All compounds were isolated for the first time from this fruiting bodies, and their effect on rat lens aldose reductase (RLAR) activity was tested. Among these eight compounds, ergosterol peroxide (3) was found to exhibit potent RLAR inhibition, its IC50 value being 15.4 microg/mL.     

Terpenoids and steroids from several euphorbiaceae and pinaceae plants]

During the course of a search for biologically active constituents from unexamined plant sources, several biogenetically interesting new di- and tri-terpenes and steroids were isolated from several weeds and shrubs of Euphorbiaceae and the bark, leaves and cones of several Pinaceae trees which had been treated as wastes in the forestry industry. Euphorbia supina contained 3,4-seco-5 alpha- and 5 beta-adian-4(23)-ene-3,5-diols and related oxides, oxygenated fern-8-en-3 beta-ols named supinenolones A-E and unusually migrated oxyfernanes having (9S)- and (9R)-7(8-->9)abeo-9-D:C-friedo-B':A'-neogammacerane skeletons named spirosupinane and neospirosupinane, while E. chamaesyce contained 3,4-seco-oleana-4(23), 18-dien-3-oic acid, 3,4-seco-8 beta H-ferna-4(23),9(11)-dien-3-oic acid and two oxygenated obtusifoliols. The bark of Phyllanthus flexuosus (Euphorbiaceae) contained 11 beta-hydroxy-D:A-friedo-olean-1-en-3-one, lup-20(29)-ene-3 beta, 15 alpha-diol, olean-12-ene-3 beta,15 alpha-diol and olean-12-ene-3 beta,15 alpha,24-triol together with trichadenic acid B for which we revised the structure to 3 beta-hydroxy-D:A-friedo-oleanan-27-oic acid. Two 26-nor-D:A-friedo-olean-14-enes were isolated from P. watsonii. Regarding Pinaceae trees, an unusually migrated abieslactone [(3R, 7S, 9R, 23R)-7-hydroxy-3-methoxy-8-oxo-7(8-->9)abeo-lanost-24-eno-26,23-lactone], named spiroveitchionolide, was isolated from the bark of Abies species, besides nine abieslactone analogues. Two pairs of unusually migrated serratanes, piceanonols A and B and jezananals A and B having novel skeletal systems of 14(13-->12) abeo- and 16(15-->14) abeo-serratanes named piceanane and jezanane, respectively, were also isolated from the stem bark of Picea species, besides three 14 beta,15 beta-epoxyserratanes and two 13 alpha,14 alpha-epoxyserratanes. The leaves of Larix kaempferi contained two deformed abietanes named karamatsuic acid (9,10-seco-9,10 alpha-epoxyabieta-8,11,13-trien-18-oic acid) and larikaempferic acid [9 alpha,13 alpha-epoxy-8-oxo-9(8-->7)abeo-7 beta-abietan-18-oic acid], as well as the cones to contain 8 alpha,12 alpha-epidioxy-15-hydroxyabiet-13-en-18-oic acid, three diepoxy-abietan-18-oic acids and two new dehydroabietic acid analogues. Several of the above compounds exhibited inhibitory effects against tumor-promoting and DNA topoisomerase II activities.     

番荔枝化学成分研究

从番荔枝(Annona squamosa)中分离得到12个化合物,分别鉴定为liriodenine(AS—1),穆坪马兜铃酰胺(moupinamide,AS-2),-(-)-kauran-16α-01-19-oic acid(AS-3),16β,17-dihydroxy-(-)-kauran-19-oic acid(AS-4),anonaine(AS-5),16α,17-dihydroxy-(-)-kauran-19-oic acid(AS-6),(-)-isokaur-15(16)-en-17,19-dioic acid(AS-7),番荔枝酰胺squamosamide(AS-8),16α-methoxy-(-)kauran-19-oicacid(AS-9),sachanoic acid(AS-10),(-)-kauran-19-al-17-oic acid(AS-11),daucosterol(AS-12)。其中,番荔枝酰胺(AS-8)是新化合物,AS-9是第一次从植物中得到的天然产物。     

Chemical constituents of Nouelia insignis Franch

Two new diterpenes and ent-15alpha-hydroxykaur-16-en-19-oic acid 11,12-acetonide (3), together with 23 known compounds were isolated from the dried aerial parts of Nouelia insignis Franch. The structures of new compounds were determined to be ent-14beta,15alpha-dihydroxykaur-16-en-19-oic acid (1), ent-14beta-hydroxy-15-oxokaur-16-en-19-oic acid (2) on the basis of spectral and chemical evidence. The structure of ent-11alpha,16alpha-epoxy-15alpha-hydroxy-16S-kaur-19-oic acid (4) was confirmed by X-ray crystallographic analysis.     

ent-Kaurenoids from the roots of Cacalia pilgeriana

Two new ent-kaurenoids,19-acetoxyl-ent-3beta,17-dihydroxykaur-15-ene (1), 19-acetoxyl-ent-3beta-hydroxykaur-15-en-17-al (2), together with seven known ent-kaurenoids: ent-kaur-16-en-19-al (3), ent-kaur-16-en-19-oic acid (4), ent-kauran-16beta,17-diol (5), ent-15beta, 16beta-epoxy-17-hydroxykauran-19-oic acid (6),19-acetyl-ent-3beta-hydroxyl-kaur-16-ene (7), ent-3beta,19-dihydroxykaur-16-ene (8), ent-17-hydroxykaur-15-ene (9), were isolated from Cacalia pilgeriana. Their structures were elucidated by spectroscopic methods including 2D NMR spectral analysis.     

Triterpenoids from the Flower of Campsis grandiflora K. Schum. as Human Acyl-CoA: Cholesterol Acyltransferase Inhibitors

The flower of Campsis grandiflora K. Schum. was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H2O. From the EtOAc fraction, seven triterpenoids were isolated through the repeated silica gel, ODS column chromatographies and preparative HPLC. From the result of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as 3beta-hydroxyolean-12-en-28-oic acid (oleanolic acid, 1), 3beta-hydroxyurs-12-en-28-oic acid (ursolic acid, 2), 3beta-hydroxyurs-12-en-28-al (ursolic aldehyde, 3), 2alpha,3beta-dihydroxyolean-12-en-28-oic acid (maslinic acid, 4), 2alpha,3beta-dihydroxyurs-12-en-28-oic acid (corosolic acid, 5), 3beta,23-dihydroxyurs-12-en-28-oic acid (23-hydroxyursolic acid, 6) and 2alpha,3beta,23-trihydroxyolean-12-en-28-oic acid (arjunolic acid, 7). These teriterpenoids were isolated for the first time from this plant. Also, compounds 4, 5, 6, and 7 revealed relatively high hACAT-1 inhibitory activity with the value of 46.2+/-1.1, 46.7+/-0.9, 41.5+/-1.3 and 60.8+/-1.1% at the concentration of 100 microg/mL, respectively.     

Six new sesquiterpenes from Cacalia ainsliaeflora

Five new eremophilane sesquiterpenes, 3beta,6beta-diangeloyloxy-8beta,10beta-dihydroxyeremophilenolide (1); 6beta-acetoxy-3beta-angeloyloxy-8beta,10beta-dihydroxyeremophilenolide (2); 3beta-angeloyloxy-6beta-methoxyeremophil-7(11),9(10)-dien-8alpha,12-olide (3), 3beta-angeloyloxy-8-oxo-eremophil-6(7)-en-12-oic acid (4); 3beta-angeloyloxy-10beta-hydroxy-8-oxo-eremophil-6(7)-en-12-oic acid (5), and a novel nor-eremophilane derivative, 3beta-angeloyloxy-10beta-hydroxy-8-oxo-eremophil-6(7)-en (6), were isolated from the roots of Cacalia ainsliaeflora. Their structures were elucidated by spectroscopic methods, including 2DNMR. Compounds 1 and 2 were assayed against P388 and A549 Carcinoma cell lines. No positive activities were observed.     

爪哇脚骨脆中三萜类化学成分研究(英文)

从大风子科植物爪哇脚骨脆的树干中分离得到11个已知三萜类化合物,经波谱解析及与文献对照方法,分别鉴定为木栓酮-2,3-内酯(1),木栓烷(2),表木栓醇(3),木栓酮(4),2α,3α,19α-三羟基-乌苏烷-12-烯-28-羧酸(5),2α,3β,19α-三羟基-乌苏烷-12-烯-28-羧酸(6),2α,3α,23-三羟基-乌苏烷-12-烯-28-羧酸(7),2α,3α,23-三羟基-齐墩果烷-12-烯-28-羧酸(8),2α,3α,19α,23-四羟基-乌苏烷-12-烯-28-羧酸(9),2α,3α,19α,23-四羟基-乌苏烷-12-烯-28-羧酸-28-O-β-D-葡萄糖酯(10),及3β,19α-双羟基-乌苏烷-12-烯-28-羧酸3-O-α-L-阿拉伯糖苷(11).其中化合物1为大风子科植物中稀有的裂环木栓烷三萜,所有化合物皆为首次从本植物中分离得到。     

Eremophilane sesquiterpenes from Ligularia myriocephala

Five new eremophilenolides, 1beta-angeloyloxy-6beta,10beta-dihydroxy-8beta-methoxyeremophil-7(11)-en-8alpha,12-olide ( 1), 1beta-angeloyloxy-6beta,10alpha-dihydroxy-8alpha-methoxyeremophil-7(11)-en-8beta,12-olide ( 2), 1beta,6beta-diangeloyloxy-8beta,10beta-dihydroxyeremophil-(11)-en-8alpha,12-olide ( 3), 1beta,6beta-diangeloyloxy-8alpha,10 alpha-dihydroxyeremophil-7(11)-en-8beta,12-olide ( 4), 1beta-angeloyloxy-8-oxoeremophil-6,9-dien-12-oic acid methyl ester ( 5) and one known compound, 8beta,10beta-dihydroxyeremophilenolide ( 6) were isolated from the extract of the whole plant of Ligularia myriocephala Ling. Their structures and stereochemistry were elucidated by various spectroscopic methods including intensive 2D-NMR techniques (COSY, gHMQC, gHMBC and (1)H- (1)H NOESY) and HR-ESI-MS. A single-crystal X-ray experiment was performed for compound 1.