二次游离氧化钙测定条件

研究了测定熟料中二次游离氧化钙的条件.结果表明:只要增大分析试样的细度,保证足够长的萃取时间,用甘油-乙醇法和乙二醇-乙醇法都能测定出熟料中的二次f-CaO,乙二醇-乙醇比甘油-乙醇萃取速度快.     

影响明胶膜性能的因素研究

用简单的手涂方法制备了明胶膜,研究了明胶溶液的浓度、pH值、增塑剂甘油的用量以及添加剂乙醇的用量对明胶膜抗拉强度的影响.研究结果表明:(1)明胶溶液浓度增加,膜的抗拉强度逐渐增大;(2)成膜液的pH值在7.0左右时,膜的抗拉强度最大;(3)随着增塑剂甘油含量的增加,膜干燥所需时间增长且抗拉强度降低;(4)乙醇用量为10%时,膜的抗拉强度最大;(5)明胶膜在不同pH值的缓冲溶液中,溶胀性能不同。     

甘油和乙醇对γ-亚麻酸生产菌被孢霉M11发酵的影响

对γ－亚麻酸生产菌 Ｍ１１进行了摇瓶实验。结果表明,在培养基中糖浓度 １００～１２０ｇ／Ｌ,乙醇浓度４ｇ／Ｌ,甘油浓度２ｇ／Ｌ条件下,细胞和油脂产量较高。     

甘油和乙醇对γ-亚麻酸生产菌M11发酵的影响

对 -亚麻酸生产菌M1 1进行了摇瓶实验。结果表明 ,在培养基中糖浓度1 0 0～ 1 2 0 g/L,乙醇浓度 4 g/L ,甘油浓度 2g/L条件下 ,细胞和油脂产量较高     

全细胞催化甘油生物合成L-果糖体系的构建

L-果糖是一种重要的稀少糖,广泛应用于食品、医药和化学合成等领域。通过构建E型马肝乙醇脱氢酶和NADH氧化酶重组大肠杆菌,再偶联醛缩酶重组大肠杆菌催化甘油合成L-果糖。对表达E型马肝乙醇脱氢酶和NADH氧化酶的菌株进行诱导条件的优化,诱导温度20℃,诱导时间16h,异丙基-β-D-硫代半乳糖苷添加浓度0.05mmol/L。通过单因素实验研究温度、pH值、湿细胞量、甘油浓度对合成L-果糖的影响,利用正交试验进行优化,优化条件为:温度40℃,pH值为9.0,湿细胞质量浓度为25g/L,甘油浓度为500mmol/L。在优化条件下得到L-果糖2.67g/L。采用全细胞转化方法以廉价的甘油为原料生产L-果糖,不仅省去了繁琐的酶纯化步骤,无须添加辅因子,而且生产成本低廉,期望为L-果糖产品绿色生产及现有技术改造提供理论参考。     

二次游离氧化钙测定条件

研究了测定熟料中二次游离氧化钙的条件。结果表明 :只要增大分析试样的细度 ,保证足够长的萃取时间 ,用甘油 乙醇法和乙二醇 乙醇法都能测定出熟料中的二次f CaO ,乙二醇 乙醇比甘油 乙醇萃取速度快。     

酿酒酵母甘油-3-磷酸脱氢酶基因沉默表达

通过构建酿酒酵母沉默表达载体PURH-SGPD1,使GPD1基因在PGK强启动子、CYC1终止子在特定区域内进行干扰和表达.酿酒酵母SY01突变菌株与出发菌株Y01发酵实验结果相比,甘油脱氢酶酶比活比Y01下降了10.52%,甘油含量下降了13.2%;乙醇量提高了12.06%.结果表明通过反义干扰GPD1基因5'UTR序列,能有效干扰酵母工程菌株GPD1转录与表达,削弱甘油积累途径,促进乙醇代谢途径.     

二次游离氯化钙测定条件

研究了测定熟料中二次游离氯化钙的条件。结果表明：只要增大分析试样的细度，保证足够长的萃取时间，用甘油－乙醇法和乙二醇－乙醇法都能测定出熟料中的二次f-CaO，乙二醇－乙醇比甘油－乙醇萃取速度快。     

甘油生物歧化过程动力学数学模拟和多稳态研究

用过量动力学模型描述了甘油生物转化的连续培养和间歇培养过程,讨论了系统中出现多稳态现象的条件和原因,并用实验证实了多稳态的存在。提出了反应器中底物浓度变化速率的概念,并用这一概念描述了毒性产物乙醇的形成。     

智能化微生物发酵乙醇浓度在线检测仪的研制

乙醇浓度是微生物发酵过程中重要的过程参数,直接影响着发酵过程的控制和优化.基于半导体材料的气敏效应,提出了一种生物发酵过程中乙醇浓度在线检测方法.该方法利用系统辨识建立乙醇浓度的在线测量模型,以微控制器为核心的嵌入式系统完成信号采集、数据分析与处理;在此基础上研制了乙醇浓度在线检测仪,给出了在线检测仪的系统组成框图以及系统的硬件设计和软件设计.实验与分析表明,所设计的乙醇浓度检测仪能够在线检测发酵过程中的乙醇浓度,所测乙醇溶液体积百分比范围为0.00-8.00%,具有较高的检测精度,绝对误差小于0.1%,重复性好,能够很好地满足微生物发酵工业应用需求.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…