萘醌溶液的吸收和荧光光谱分析

研究了萘醌的吸收光谱特性和紫外光激励下的荧光光谱特性,分析了吸收光谱和荧光光谱随萘醌浓度改变的变化规律及两种光谱技术检测萘醌的工作曲线和检测限。结果显示,萘醌溶液对波长245 nm的紫外光有强烈的吸收,在331 nm处有次吸收峰出现,且吸收峰的强度随溶液浓度的降低(10→2μM)呈线性衰减。萘醌吸收光后有较好的荧光发射性能,在250~600 nm波段有明显的荧光发射,荧光峰位于423 nm处。最佳激励波长为306 nm,荧光峰的强度随溶液浓度的变化(10→2μM)呈线性分布。萘醌浓度梯度的吸收和荧光光谱分析结果显示,两种检测方法对于萘醌的检测限均为2μM。     

高效液相色谱-柱后衍生荧光法同时检测粮食中8种氨基甲酸酯类农药

建立了高效液相色谱-柱后衍生荧光法同时检测粮食中8种氨基甲酸酯类农药,样品经乙腈提取,SPE-NH_2小柱净化,减压旋转蒸发浓缩后定容.检测系统为柱后光化学衍生器串联荧光检测器,激发波长330 nm,发射波长465 nm.外标法定量,方法的线性范围为0.01～1.00 mg·L~(-1),检出限(3S/N)为3～5μg·kg~(-1),用该方法检测大米、小麦和玉米等试样,加标回收率介于77.9%～106.3%,相对标准偏差不大于6.7%(n=6).     

SDS的荧光光谱及其寿命

十二烷基硫酸钠(SDS)是一种重要的荧光增敏剂,对其荧光特性的研究具有重要应用价值.利用FLS920荧光光谱仪具体测量不同浓度SDS水溶液的荧光及寿命,发现SDS的荧光光谱有如下特征:低浓度时仅有一个荧光峰,在290～300 nm左右,浓度增大后在330 nm处出现第二个峰.第一荧光峰应由碳硫间氧原子激发产生;第二荧光峰则由与硫键合的氧原子激发产生.还对其衰减曲线进行拟合,计算了荧光平均寿命,发现SDS荧光平均寿命随浓度增加呈变大趋势,与330 nm处荧光峰相比,300 nm处荧光平均寿命较小.     

一种新型硫化氢近红外比率荧光探针的研制

设计合成了一种基于花色素染料的近红外比率荧光探针分子1,并将其用于活细胞内内源性和外源性硫化氢的检测.结果表明:探针分子1溶液中加入硫化氢时,因硫化氢对探针分子1中苯并吡喃部分的亲核加成破坏了其共轭体系,导致720 nm处的近红外(NIR)发射荧光强度降低.同时,其加成产物含有完整的半刚性香豆素荧光团,在503 nm处的绿色荧光强度显著增强.由于探针1的发射波长与半刚性香豆素的发射波长不同,因此在加入硫化氢前后有两个完全分离的发射峰,实现了对硫化氢的有效比率检测.当加入4.0μmol·L~(-1)硫化钠时,荧光强度增强了19.2倍.探针分子1对水溶液中的硫化氢的线性响应范围是0.1～4.0μmol·L~(-1)(R~2=0.993 9),检测下限为57.8 nmol·L~(-1).探针分子1对硫化氢比较敏感,响应时间为110 s,且有较好的选择性.此外,探针分子1还被成功应用于活细胞中内源性和外源性硫化氢的荧光成像.     

甲苯胺蓝共振瑞利散射光谱法测定硫酸皮肤素

在适宜pH条件下,甲苯胺蓝与硫酸皮肤素作用形成结合产物时将导致溶液共振瑞利散射(RRS)明显增强并产生新的RRS光谱,最大散射峰位于356.0 nm处.硫酸皮肤素在0.1～4.0 μg·mL-1范围内与RRS强度成正比;检出限(3σ)为 8.5 × 10-2 μg·mL-1.本文研究了适宜的条件和影响因素,考察了共存物质的影响.实验证明,该方法有较好的选择性和灵敏性;应用于合成样品的测定,结果令人满意.     

白芍水萃取液的荧光光谱研究

采用荧光光谱分析技术,研究了白芍水萃取液在230-260nm紫外光激发下的荧光光谱。研究结果表明,在245nm波长处产生的荧光较强,荧光峰是280-405nm范围的宽谱峰,荧光峰值波长在320nm处;同时采用245nm紫外光激发不同浓度的白芍水萃取液时,最大荧光相对强度和溶液浓度之间存在较好的线性关系,为检测白芍有效成分的含量提供了一种新的方法。该研究结果对推动光谱分析技术在中草药分析研究中的应用将起到重要的作用。     

Se(IV)与藻蓝蛋白相互作用的谱学研究

用吸收、荧光、红外等谱学方法研究了藻蓝蛋白(PC)与Se(IV)的相互作用.结果表明,SeO2-3加入后,藻蓝蛋白在620nm处的特征光吸收减弱,并随Se(IV)浓度和时间的增加而降低,而278和347nm处的光吸收增强;荧光发射和荧光激发也逐渐减弱,而599和629nm处的2个激发峰的相对强度与对照相反.PC在475和662nm处分别出现共振散射峰.Se(IV)与PC的作用后,在595nm处出现强的共振散射峰,被指认为液相纳米硒粒子与PC生成的缀合物的共振散射峰.红外光谱图中,PC的酰胺I带为1653 2cm-1,为α螺旋,而PC-Se(0)生物缀合物的酰胺I带为1647 0cm-1,属无规则卷曲.     

没食子蓝荧光探针法测定DNA的研究

没食子蓝在239nm紫外光激发下，在414nm处出现一个小的荧光峰，加入小牛胸腺脱氧核糖核酸(ctDNA)，该荧光峰的强度大大增强．其荧光增强比值与ctDNA的浓度分别在低浓度区问(0．05—1mg／L)和稍高浓度区间(1—10mg／L)呈较好的线性关系，利用这一原理建立了一种用没食子蓝作为荧光探针测定DNA的方法．该方法相当简单，其检测限可达0．04mg/L．     

水热法合成YVO4:Eu3+荧光粉及其发光性能研究

以氧化钇和氧化铕为原料,采用简单的水热法合成YVO4:Eu3+荧光粉,利用XRD、SEM、FTIR和荧光光谱仪对其结构、形貌及其性能进行表征.XRD结果表明:产物为四方晶系YVO4:Eu3+.SEM显示样品为不规则棉花团状.探讨了水热反应温度、反应时间、p H、表面活性剂等因素对产物荧光性能的影响。用波长为283 nm的近紫外光激发,荧光粉的特征发射峰分别对应于Eu3+的5D1→7F1(539nm),5D0→7F1(596nm),5D0→7F2(619nm)电子跃迁.最强发射峰位于619nm处,属于Eu3+的特征红光发射.     

Nd~(3+)∶KLa(MoO_4)_2激光晶体的光谱特性分析

采用中频感应提拉法生长出了Nd3+∶KLa(MoO4)2单晶,测试其室温下非偏振吸收光谱、荧光发射光谱.805 nm处吸收峰半峰宽10 nm,吸收截面为3.74×10-20cm2.主荧光发射峰位于1 059.45 nm处,荧光寿命160.6μs.利用Judd-Ofelt理论计算出J-O强度参数Ωt(t=2,4,6)及自发辐射概率、荧光分支比、辐射寿命、荧光量子效率及受激发射截面等参数.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.