乙型肝为病毒（HBV）DNA免疫的初步研究

用聚合酶链反应（ＰＣＲ）扩增了编码ＨＢＶｓＡｇ的基因序列,将其插入到ｐｃＤＮＡ３载体中,位于人巨细胞病毒（ＣＭＶ）早期启动子下游,重组质粒ｐｃＤＮＡ３－ｓＡｇ转染细胞后检测到ＨＢｓＡｇ表达,用纯化后的重组质粒直接注射用ＢＡＬＢ／Ｃ小鼠骨骼细内,诱发实验小鼠产生了抗ＨＢｓＡｇ特异性抗体,ＰＣＲ扩增未检测到注射部位及肝脏细胞染色体中有外源ＨＢＶＤＮＡ整合。     

乙型肝炎病毒(HBV)DNA免疫的初步研究

用聚合酶链反应（ＰＣＲ）扩增了编码ＨＢＶｓＡｇ的基因序列,将其插入到ｐｃＤＮＡ３载体中,位于人巨细胞病毒（ＣＭＶ）早期启动子下游．重组质粒ｐｃＤＮＡ３－ｓＡｇ转染细胞后检测到ＨＢｓＡｇ表达．用纯化后的重组质粒直接注射到ＢＡＬＢ／Ｃ小鼠骨骼肌内,诱发实验小鼠产生了抗ＨＢｓＡｇ特异性抗体．ＰＣＲ扩增未检测到注射部位及肝脏细胞染色体中有外源ＨＢＶＤＮＡ整合     

信息桥

单　　位 :北京大学医学部实验动物科学部地　　址 :北京海淀学院路　 10 0 0 83联系电话 :0 10 - 6 2 0 9135 0 ;13910 735 12 0联系人 :张淑英北京大学医学部实验动物科学部产品价目表品种品　系级别价格 元大鼠Ｗｉｓｔａｒ 115元大鼠Ｗｉｓｔａｒ(孕鼠 ) 140元大鼠Ｗｉｓｔａｒ(乳鼠 ) 14元大鼠ＲＣＳ视网膜变性模型1230元50元大鼠Ｐ77癫痫模型 130元大鼠ＬＥＷ1230元50元大鼠ＳＤ1215元2 0元小鼠ＫＭＫＭ(孕鼠 )ＫＭ(乳鼠 )ＫＭ11123元2 5元2元5元小鼠ＬＡＣＡ124元6元小鼠ＢＡＬＢ ｃＢＡＬＢ ｃ(孕鼠 )117元30元品种品　系级别价格 …     

秋水仙碱对斑马鱼肝脏和鳃组织中SOD及Na~+-K~+-ATPase活性的影响

将斑马鱼(Brachyclanio rerio)暴露于秋水仙碱5个浓度组(0mg·mL-1、10.00mg·mL-1、14.12mg·mL-1、19.95mg·mL-1、28.18mg·mL-1、39.80mg·mL-1)中进行96h急性毒性试验,计算其半数致死浓度(LC50),再设置秋水仙碱3个浓度(0.67mg·mL-1、1.70mg·mL-1、4.26mg·mL-1)将斑马鱼进行21d慢性毒性实验,每7天测定肝脏和鳃中的超氧化物歧化酶(SOD)及N+-K+-三磷酸腺苷酶(Na+-K+-ATPase)活性。结果表明,秋水仙碱对斑马鱼的LC50为16.90mg·mL-1,随着秋水仙碱浓度增大和染毒时间延长,斑马鱼鳃中SOD和Na+-K+-ATPase活性均显著受到抑制,肝脏中SOD活性增加,Na+-K+-ATPase活性总体呈现抑制趋势。斑马鱼肝脏和鳃中的SOD及Na+-K+-ATPase对秋水仙碱敏感,可以作为观测指标用于评价秋水仙碱的毒性。     

离子色谱法测定金矿样中的二价金属离子

实验采用ＨＩＣ 6Ａ离子色谱仪 (日本岛津 ) ,ＣＣＤ 6Ａ电导检验器 ,Ｃ Ｒ5Ａ微型数据处理机 (日本岛津 )。淋洗液 :先配制成 4 0ｍｍｏｌ/Ｌ酒石酸和 2 0ｍｍｏｌ/Ｌ乙二胺的贮备液 ,使用时用去离子水稀释 10倍 ,酒石酸和乙二胺均为ＡＲ级以上。标准液 :分别用ＡＲ级以上的ＺｎＣｌ2 、ＣａＣｌ2 、ＭｇＣｌ2 和Ｓｒ(ＮＯ3) 2 配制成 1.0ｍｇ/ｍＬ的贮备液 ,使用时按所需浓度稀释。去离子水电导率须小于 1.0 μｓ·ｃｍ- 1。Ｓｈｉｍ ＰａｃｋＩＣ ＣＩ5× 15 0ｍｍ阳离子分离柱 ,进样体积 10 μＬ ,淋洗液流量 1ｍＬ/ｍｉｎ ;832 μｓ /…     

鳜鱼和大眼鳜鱼年龄生长和繁殖力的比较研究

为了进一步改善鳜养殖的良种供应和原、良种生产及制定种质标准 ,我们对鳜及大眼鳜的年龄生长与繁殖力等做了比较研究 ,现将结果报告如下 .1　材料和方法　　鳜是南海鳜鱼良种场于 2 0 0 0 - 2 0 0 2年间从鄱阳湖购进的天然水域的野生种 ,购买时即进行形态学测量 ,计数可数性状 ;大眼鳜采集于北江水域 .测量鳜 30 0尾体长在 9 8- 4 3 5ｃｍ之间 ,体质量在 32 - 1 44 1ｇ之间 ;大眼鳜 2 80余尾体长在 6 7- 34 6ｃｍ之间 ,体质量在 2 0 -1 1 37ｇ之间 .年龄测定用测鳃盖骨观察关节突中点至边缘年龄带数 .年龄和生长的分析计算公式[1-4 ] :①…     

北京大学医学部实验动物科学部动物品种品系一览表

ＬａｂｏｒａｔｏｒｙＡｎｉｍａｌＭａｉｎｔａｉｎｅｄｂｙＤｅｐａｒｔｍｅｎｔＯｆＬａｂ .Ａｎｉｍａｌ,ＨｅａｌｔｈＳｃｉｅｎｃｅＣｅｎｔｅｒ,ＰｅｋｉｎｇＵｎｉｖｅｒｓｉｔｙ品系名称(Ｓｔｒａｉｎ)等级(Ｑｕａｌｉｔｙ)遗传类别(Ｔｙｐｅ)备注(Ｒｅｍａｒｋ)ＭｉｃｅＫ .Ｍ 1、2远交ＬＡＣＡ 1、2远交ＢＡＬＢ/Ｃ 1、2、3近交Ｃ57ＢＬ/ 6Ｊ 1、2近交ＤＢＡ/ 2 2近交ＩＣＲ 3远交ＢＸＳＢ 2远交红斑狼疮模型Ｓｃｉｄ 3近交Ｔ、Ｂ联合缺陷小鼠ＮｕｄｅＭｉｃｅＩＣＲ ｎｕ 3远交Ｔ细胞缺陷裸小鼠ＢＡＬＢ/Ｃ ｎｕ 3远交Ｔ细胞缺…     

Adaptive Lattice Boltzmann Model for Compressible Flows

ＩｎｔｒｏｄｕｃｔｉｏｎＩｎｒｅｃｅｎｔｙｅａｒｓ,ｔｈｅＬａｔｔｉｃｅＢｏｌｔｚｍａｎｎ(ＬＢ)ｍｅｔｈｏｄｈａｓａｔｔｒａｃｔｅｄｍｕｃｈａｔｔｅｎｔｉｏｎａｓａｎｏｖｅｌａｌｔｅｒｎａｔｉｖｅｔｏｔｒａｄｉｔｉｏｎａｌｍｅｔｈｏｄｓｆｏｒｎｕｍｅｒｉｃａｌｌｙｓｏｌｖｉｎｇｔｈｅＮＳｅｑｕａｔｉｏｎｓ[1].ＴｈｉｓｍｅｔｈｏｄｏｒｉｇｉｎａｔｅｄｆｒｏｍａＢｏｏｌｅａｎｍｏｄｅｌｋｎｏｗｎａｓＬａｔｔｉｃｅＧａｓＡｕｔｏｍａｔａ(ＬＧＡ)[2].ＴｈｅｓｔａｎｄａｒｄＬＧＡｍｏｄｅｌｉｍｐｏｓｅｓ,ｆ…     

Positive Singular Solutions for Quasilinear Elliptic Equations With Supercritical Growth

Ｉｎｔｈｉｓｐａｐｅｒｗｅｓｔｕｄｙｔｈｅｅｘｉｓｔｅｎｃｅｏｆｐｏｓｉｔｉｖｅｓｉｎｇｕｌａｒｓｏｌｕｔｉｏｎｓｏｆｔｈｅｆｏｌｌｏｗｉｎｇｐｒｏｂｌｅｍｓｄｉｖ(｜ ｕ｜ｐ- 2 ｕ) +ｆ(ｕ) =0　　ｉｎ　Ω \{ 0 } (1)ｌｉｍｘ→ 0 ｕ(ｘ) =∞ . (2 )Ｗｈｅｒｅ 1<ｐ <Ｎ ,Ω =ＢＲ ={ｘ∈ＲＮ:｜ｘ｜<Ｒ}ｉｓａｆｉｎｉｔｅｂａｌｌｏｒΩ=Ｂ∞ =ＲＮ,Ｎ 3.Ｔｈｅｎｏｎｌｉｎｅａｒｆｕｎｃｔｉｏｎｆ∈Ｃ1(Ｒ) (ｏｒｆｉｓｉｎｇｅｎｅｒａｌｌｏｃａｌｌｙＬｉｐｓｃｈｉｔｚｃｏｎｔｉｎｕｏｕｓ)ｓａｔｉｓｆｉｅｓ…     

JOURNAL OF SICHUAN UNIVERSITY (Natural Science Edition)Bimonthly Vol.38 No.1 Sum Contens Dec.2001

ＭａｔｈｅｍｔｉｃｓａｎｄＣｏｍｐｕｔｅｒＳｃｉｅｎｃｅＯｎｔｈｅＤｉｏｐｈａｎｔｉｎｅＥｑｕａｔｉｏｎｘ2 -Ｄｙ4 =1ＬＵＯＪｉａ ｇｕｉ,ＹＵＡＮＰｉｎｇ ｚｈｉ (5 )……………………………………………………ＡＮｏｔｅｏｎＡｒｔｉｃｅｏ ＭｏｒｅｓｃｏＦｕｚｚｙＰｒｏｘｉｍｉｔｙＳｐａｃｅｓ ＬＩＳａｎ ｊｉａｎｇ ,ＬＵＯＭａｏ ｋａｎｇ (6 )…………………………………………ＤｅｔｅｃｔｉｏｎｏｆＳｔｅｐ ＳｔｒｕｃｔｕｒｅＥｄｇｅＢａｓｅｄｏｎＯｒｄｅｒＳｔａｔｉｓｔｉｃＦｉｌｔｅｒＭＡＨｏｎｇ ,ＹＵ…     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

The Analects and the Moment in Peking——The influence of a Confucian education system on the personality formation,the way of thinking and the fate of the main characters

0.Preface China has long been boasting of its colorful and immense culture which comprises of Taoism,Confucianism and Buddhism.The interwoven influence among the religions on Chinese way of thinking,on the nation＇s behavior in their social life throughout the long history is beyond list.