混菌固态发酵啤酒糟的研究

利用不同原料配比进行混菌发酵啤酒糟来提高其蛋白质量分数研究.研究表明,利用木霉、黑曲霉和酵母混菌发酵,啤酒糟和麦夫皮配比为4∶1时,在28-30 ℃,含水量65%-70%条件下,发酵3 d后蛋白质质量分数提高到35%以上.     

生物转化啤酒糟为饲料蛋白的研究

利用对黑曲霉、白地霉、热带假丝酵母、产朊假丝酵母及添加纤维素酶混合发酵啤酒糟生产饲料蛋白质进行了初步的研究.正交试验结果表明,最佳原料配比为 m(啤酒糟 )∶ m(麸皮 )∶ m(豆粕 )=80∶ 15∶ 10,原料含水量为 60％,其最佳发酵条件为发酵温度 30℃ ,pH为 6.0,接种量为 2％,培养时间 3 d. 成分分析结果表明,发酵产物营养丰富,粗蛋白含量达 41.52％.     

酸性蛋白酶饲料固态发酵条件的研究

探讨了以啤酒糟为主要原料进行固态发酵生产酸性蛋白酶饲料的方法,研究了不同发酵基质配比及发酵条件对酸性蛋白酶活力的影响.试验结果表明,最适的发酵基质配比为m(啤酒糟)∶m(豆粕)∶m(玉米淀粉)∶m(KH2PO4)=100∶9∶2∶0.2;最适发酵条件为:原料含水量为58%,发酵温度31℃,初始pH为5.5,发酵时间84 h.在上述条件下,酸性蛋白酶活力达7 800 U/g.     

发酵啤酒糟生产饲料蛋白的研究

利用热带假丝酵母（Ｃａｎｄｉａｔｒｏｐｉｃａｌｉｓ）、绿色木霉（Ｔｒｉｃｈｏｄｒｍａｌｉｇｎｏｒｕｍ），白地霉（ＧｒｉｅｏｔｒｉｃｈｕｍｃａｎｄｉｄｕｍＬｉｎｋ）、黑曲霉（ＡｓｐｅｒｇｉｌｌｕｓＮｉｇｅｒ）、米曲霉（Ａｓｐｅｇｉｌｌｕｓｏｒｙｚａｌ）等五株菌混合发酵啤酒糟生产饲料蛋白，提高饲料的利用价值，并改善了啤酒糟的适口性。通过五种菌株的协同作用使啤酒糟中的非氮物质转化为蛋白质，没被利用的多糖物质和如纤维素，半纤维素及部分淀粉转化为被消化吸收的低聚糖、双糖和单糖。在同一条件下，同一时间内各种菌株相互间协调，各自完成发酵的功能。培养基为啤酒糟８５％、玉米面１０％、麦麸５％。培养温度２８－３０℃，培养周期４８ｈ。分析结果表明：粗蛋白含量高达３２．８６％以上，氨基酸齐备，粗纤维降低到９．９％，具有较高的蛋白酶，果     

啤酒糟蛋白曲奇饼干的研究

为了提高啤酒糟的利用价值和应用范围,将啤酒糟蛋白作为原料部分替代面粉制备了曲奇饼干,并进行感官评价和质构分析实验。结果表明啤酒糟蛋白曲奇饼干的最佳配方为:面粉和啤酒糟蛋白合计100%,鸡蛋50%,黄油45%,糖粉20%,其中啤酒糟蛋白量占面粉和啤酒糟蛋白总量比的15%。口感酥脆,麦香味怡人,适于工业生产。     

黑曲霉和热带假丝酵母混菌固态发酵麸皮工艺优化

为探索微生态发酵饲料固态发酵的最佳工艺参数,以黑曲霉和热带假丝酵母作为发酵菌种,以麸皮作为主要发酵基质,进行混菌发酵,以提高麸皮的蛋白含量和减少麸皮的粗纤维含量为衡量指标,优化工艺参数.最佳工艺参数为:麸皮含水量70%,黑曲霉与热带假丝酵母菌种配比为2∶1,发酵温度32℃、发酵时间72h.在此工艺参数下固态发酵麸皮发酵效果最好,蛋白质含量最高,粗纤维含量最低.     

多菌联合固态发酵生产酒糟菌体蛋白饲料的实验研究

利用米曲霉、黑曲霉和酵母组成的三菌发酵体系对啤酒糟进行高蛋白菌体饲料化生物转化.方法为以预处理过的酒糟粉为主要培养基,添加少许辅料,接种上述三种菌种进行固态发酵.该方法的培养周期为6d,所得酒精菌体蛋白饲料的粗蛋白含量高达35.9%,糖化酶活力达7564.1u/g,演粉酶活力达4824.4.     

利用啤酒糟液体深层培养风尾菇产木聚糖酶的初步研究

研究了风尾菇（Pleurotus ostreatus）利用啤酒糟作为原料进行液态发酵产木聚糖酶的可行性.通过正交试验得出最佳的培养基配方.同时对不同发酵时间还原糖的减少和木聚糖酶活性的进行测定.培养基最佳配方：啤酒糟6 g,黄豆粉0.32 g,玉米粉2 g,糖10 g,pH值5.0.发酵的最佳时间为72 h,此时木聚糖酶的活性最大,为9.2 U/mL.     

白酒丢糟的多菌种混菌固态发酵制备有机肥

以白酒丢糟为主要原料,4种芽孢杆菌、2种霉菌与酿酒酵母为混菌发酵菌株,进行多菌种混菌固态发酵,以发酵产物的腐殖质、全氮、全磷、全钾含量为指标,通过单因素实验和响应面法分析探讨酒糟固态发酵制备有机肥在初始含水量、酒糟量和初始pH的最佳工艺条件.经验证性实验可知预测值与实际值相符,得到最佳工艺条件:初始含水量为60.54%、酒糟量为52.06%、初始pH为7.03,接种量为3%~5%,发酵时间为6 d,发酵产物中腐殖质含量达到17.50%,种子发芽指数GI值达到93.2%,E4/E6值为2.19.     

谷氨酸发酵废醪菌渣营养分析及其RNA的提取

以豆粕、花生粕、棉籽粕为对照,测定了谷氨酸发酵废醪菌渣的主要营养成分,并用浓盐法提取其RNA。结果表明,谷氨酸发酵废醪菌渣蛋白质质量分数为430 g/kg,仅次于豆粕,高于花生粕和棉籽粕;游离氨基酸和赖氨酸含量明显高于3个对照样品,分别为对照样品的6~7倍和7~8倍;游离氨基酸包括必需氨基酸赖氨酸(Lys)、缬氨酸(Val)和亮氨酸(Leu)等5种,而对照样品中游离氨基酸只2~3种;粗纤维、粗脂肪和总糖含量明显均低于对照样品;谷氨酸发酵废醪菌渣中RNA质量分数为68.8 g/kg,高于啤酒酵母菌。提取得到的RNA制品纯度达到67.7%,与市售酵母RNA纯度相近。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.