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外泌体是细胞主动分泌的一种纳米级双层膜结构的小囊泡,能够直接反映分泌细胞的生理和功能状态,进行细胞间的物质运输和信息通讯,并参与多种生理及病理过程.本文针对细胞分泌外泌体的动态过程,以DNA四面体为基础,结合细胞膜修饰技术和荧光成像技术,构建了一种易操作、高稳定性的细胞膜表面DNA四面体传感器,应用于不同细胞类型分泌外泌体的实时监测.DNA四面体传感器通过脚支链与疏水性胆固醇探针的杂交互补作用锚定于细胞膜表面,利用四跨膜蛋白CD63核酸适配体序列特异性捕获细胞膜表面释放的外泌体,通过监测细胞膜表面荧光的变化,实时测定外泌体的分泌情况. 相似文献
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The performance of terahertz optical asymmetric demultiplexer (TOAD) has been studied by modelling the semiconductor optical amplifier (SOA) in which the intraband effects had been taken into account. Numerical results are coincident with the experiment results. We interpret why there are three peaks in the switching window, which has never been reported before. In addition, we put forward the definition of the flatness of the switching window of TOAD for the first time By analysing the different phase of clockwise and counter clockwise signal pulse changed by SOA, appropriate peak power of control pulse and wavelength of signal and control pulse have been calculated in order to obtain large output power and flat switching window of TOAD. 相似文献
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