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荧光素汞在碱性条件下具有很强的荧光,H2S能与荧光素汞结合,使其荧光猝灭,据此建立了一种荧光法测定大鼠肠灌流液中H2S含量的方法。在0.1mol·L-1 NaOH溶液中,以Na2S作为H2S供体,荧光素汞浓度为5.0×10-5 mol·L-1,Na2S浓度为1.0×10-5 mol·L-1时,以498nm为激发波长,在522nm处测定此二元体系的荧光强度。结果表明,在4.0×10-7~2.0×10-6 mol·L-1范围内,H2S浓度与荧光强度的下降程度呈良好的负相关性,r=0.998 0,精密度实验RSD=4.59%(n=7),检出限3.5×10-8 mol·L-1,样品中H2S含量分别为1.01×10-6和1.15×10-6 mol·L-1,加标回收率为95.8%~101.0%。该方法操作简单,灵敏度高,稳定性好,可准确测定肠灌流液中H2S含量,为内源性H2S的测定提供了依据。 相似文献
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应用基于超高效液相色谱-串联质谱(UPLC-MS/MS)技术的代谢组学方法检测2型糖尿病患者血清胆汁酸代谢谱,获得代谢标志物,发掘可预测糖尿病的潜在预防治疗靶点。采用Waters ACQUITY UPLC BEH C18色谱柱(150 mm×2.1 mm,1.7 μm)分离,0.05%乙酸水溶液-乙腈为流动相,以0.40 mL/min流速进行梯度洗脱,用此方法测定了30例糖尿病患者和 50例正常人血清胆汁酸代谢谱。41种胆汁酸标准品在30 min内均得到良好分离,各成分在一定浓度范围内呈良好线性关系,检出限(LOD)为0.24~7.81 nmol/L,定量下限(LOQ)为0.49~15.63 nmol/L,相关系数(r2)为 0.986 7~0.999 7。血清中胆汁酸代谢谱与两组间存在相关性,通过正交偏最小二乘法-判别分析(OPLS-DA)发现,有12种胆汁酸(包括甘氨熊脱氧胆酸、甘氨胆酸、牛磺鹅脱氧胆酸、胆酸、熊去氧胆酸、甘氨鹅脱氧胆酸、甘氨脱氧胆酸、12 酮基石胆酸、鹅脱氧胆酸、脱氧胆酸、异石胆酸、石胆酸)承载了分组的重要信息。结合受试者工作特征(ROC)分析,最终获得牛磺鹅脱氧胆酸(AUC=0.68)、甘氨鹅脱氧胆酸(AUC=0.66)、甘氨熊脱氧胆酸(AUC=0.63)、胆酸(AUC=0.63)、脱氧胆酸(AUC=0.63)、甘氨脱氧胆酸(AUC=0.58) 6种胆汁酸代谢标志物,对于糖尿病的早期诊断及深入研究具有潜在的科研及临床价值。 相似文献
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Zheng Zhang · Xu Han · Chao Jiang State Key Laboratory of Advanced Design Manufacturing for Vehicle Body College of Mechanical Vehicle Engineering Hunan University Changsha China 《Acta Mechanica Sinica》2011,27(5):757-766
In this article,an effective technique is developed to efficiently obtain the output responses of parameterized structural dynamic problems.This technique is based on the conception of reduced basis method and the usage of linear interpolation principle.The original problem is projected onto the reduced basis space by linear interpolation projection,and subsequently an associated interpolation matrix is generated.To ensure the largest nonsingularity,the interpolation matrix needs to go through a timenode choosing process,which is developed by applying the angle of vector spaces.As a part of this technique,error estimation is recommended for achieving the computational error bound.To ensure the successful performance of this technique,the offline-online computational procedures are conducted in practical engineering.Two numerical examples demonstrate the accuracy and efficiency of the presented method. 相似文献
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Chun-Chuan Liu · Feng-Ming Li · Ting-Wei Liang · Wen-Hu Huang P.O.Box School of Astronautics Harbin Institute of Technology Harbin China 《Acta Mechanica Sinica》2011,27(5):785-795
In this paper,wave and vibratory power transmission in a finite L-shaped Mindlin plate with two simply supported opposite edges are investigated using the wave approach.The dynamic responses,active and reactive power flow in the finite plate are calculated by the Mindlin plate theory (MPT) and classic plate theory (CPT).To satisfy the boundary conditions and continuous conditions at the coupled junction of the finite L-shaped plate,the near-field and far-field waves are entirely contained in the wave approach.The in-plane longitudinal and shear waves are also considered.The results indicate that the vibratory power flow based on the MPT is different from that based on the CPT not only at high frequencies but also at low and medium frequencies.The influence of the plate thickness on the vibrational power flow is investigated.From the results it is seen that the shear and rotary inertia correction of the MPT can influence the active and reactive power at the junction of the L-shaped plate not only at high frequencies but also at low and medium frequencies.Furthermore,the effects of structural damping on the active and reactive power flow at the junction are also analyzed. 相似文献
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Long-He Xu · Zhong-Xian Li · Jia-Ru Qian School of Civil Engineering Beijing Jiaotong University Beijing China School of Civil Engineering Tianjin University Tianjin China Department of Civil Engineering Tsinghua University Beijing China 《Acta Mechanica Sinica》2011,27(3):399-405
A two-stage damage detection approach is proposed and experimentally demonstrated on a complicated spatial model structure with a limited number of measurements. In the experiment,five known damage patterns,including 3 brace damage cases and 2 joint damage cases,were simulated by removing braces and weakening beam-column connections in the structure. The limited acceleration response data generated by hammer impact were used for system identification,and modal parameters were extracted by using the eigensystem realization algorithm. In the first stage,the possible damaged locations are determined by using the damage index and the characteristics of the analytical model itself,and the extent of damage for those substructures identified at stage I is estimated in the second stage by using a second-order eigen-sensitivity approximation method. The main contribution of this paper is to test the two-stage method by using the real dynamic data of a complicated spatial model structure with limited sensors. The analysis results indicate that the two-stage approach is ableto detect the location of both damage cases,only the severity of brace damage cases can be assessed,and the reasonable analytical model is critical for successful damage detection. 相似文献
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Li Yin · Qin Qian · Lin Wang Huazhong University of Science Technology Wuhan China Hubei Key Laboratory for Engineering Structural Analysis Safety Assessment China 《Acta Mechanica Sinica》2011,27(3):445-451
We present a new analytical model for electrostatically actuated microbeams to explore the size effect by using the modified couple stress theory and the minimum total potential energy principle. A material length scale parameter is introduced to represent the size-dependent characteristics of microbeams. This model also accounts for the nonlinearities associated with the mid-plane stretching force and the electrostatical force. Numerical analysis for microbeams with clamped-clamped and cantilevered conditions has been performed. It is found that the intensity of size effect is closely associated with the thickness of the microbeam,and smaller beam thickness displays stronger size effect and hence yields smaller deffection and larger pull-in voltage. When the beam thickness is comparable to the material length scale parameter,the size effect is significant and the present theoretical model including the material length scale parameter is adequate for predicting the static behavior of microbeam-based MEMS. 相似文献
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Cheng-Kang Wu · Hai-Xing Wang · Xian Meng · Xi Chen · Wen-Xia PanInstitute of Mechanics Chinese Academy of Sciences Beijing China School of Astronautics Beijing University of Aeronautics Astronautics Beijing China Department of Engineering Mechanics Tsinghua University Beijing China 《Acta Mechanica Sinica》2011,27(2):152-163
The aerodynamic aspects of indirect thrust measurement by the impulse method have been studied both experimentally and numerically. The underlying basic aerodynamic principle is outlined, the phenomena in subsonic, supersonic and arc-heated jets are explored, and factors affecting the accuracy of the method are studied and discussed. Results show that the impulse method is reliable for indirect thrust measurement if certain basic requirements are met, and a simple guideline for its proper application is given. 相似文献
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