慢性关节炎和正常大鼠软骨及滑膜基因表达的差异

背景：原发性骨关节炎被确认为是一种多基因疾病。采用cDNA微阵列技术，从基因表达水平在同一载体上同时进行多基因检测，从而考察骨关节炎的滑膜与软骨基因表达类型，有助于进一步认识骨关节炎相关发病机制及为基因治疗提供依据。 目的：通过比较骨性关节炎和正常关节的软骨与滑膜的基因谱变化，筛选出骨性关节相关的差异表达基因，并探讨其在骨性关节炎发病机制中的意义及骨性关节炎的基因多态性。 方法：Wistar大鼠24只，随机分为模型组和对照组，每组12只。提取模型组和对照组的膝关节软骨与滑膜细胞，提取总RNA。用含588个基因的cDNA芯片进行微阵列基因检测，数据进行基因差异表达和聚类分析。 结果与结论：骨性关节炎大鼠及正常大鼠软骨及滑膜的基因差异表达分析结果显示，骨关节炎大鼠软骨与滑膜中的差异表达基因共有82个，其中上调的基因有27个，下调的基因有55个。基因芯片技术能有效地筛选出骨关节炎差异表达的基因并可发现新的相关基因。骨关节炎的发病涉及多种基因的表达异常，筛选到的差异表达基因将为进一步研究骨关节炎发病机制和致病相关基因功能提供理论依据。

Synovium and cartilage gene expression in chronic arthritis versus normal rats

BACKGROUND: Primary osteoarthritis (OA) is a multigenic disease. Using cDNA microarray, multigenic detection is performed on one carrier to explore gene expression type of cartilage and synovium, which provides evidence for related mechanism and gene therapy. OBJECTIVE: To compare synovium and cartilage gene expression profile of OA rats and to evaluate the potential OA related discriminating genes and their roles in pathogenesis of OA. METHODS: A total of 24 male Wistar rats were randomly divided into model and control groups with 12 animals in each group. Synovial and cartilage tissue were obtained from two groups. Total RNA was extracted from the tissues. cDNA microarray chips were used to identify gene expression profile. Differential expression and clustering analysis were performed. RESULTS AND CONCLUSION: Total gene differential expression in synovial tissue and cartilage of OA showed a high relativity. Among the target and cartilage genes, 82 differentially expressed genes were identified in OA rats, including 27 up-regulated genes and 55 down-regulated genes. Gene microarray technique is effective for screening associated genes, which helps to understand the pathogenesis of OA, and find the new genes associated with inflammation. OA is an immunogenic disorder with many abnormal expression genes. Genes screened from the target can provide important information for further study and genetic treatment of OA.