相思子毒素P2的体内外抗肿瘤作用研究

目的:研究相思子毒素P2(abrinP2)在小鼠体外和体内的抗肿瘤活性。方法:MTT体外试验法检测abrinP2对体外多种人肿瘤细胞的生长抑制作用;体内抗肿瘤试验观察abrinP2对小鼠肿瘤的抑制作用及对体重,胸腺、脾脏指数的影响。结果:MTT结果显示abrinP2对人鼻咽癌细胞CNE-2Z、人肺癌细胞A-549、人口腔上皮细胞KB、人胃癌细胞BGC均有较强的抑制作用。体内灌胃给予不同剂量的abrinP2(0.05mg/kg、0.075mg/kg和0.1mg/kg),共10天,对小鼠肿瘤Lewis、EMT6均有较强的抑制作用,抑制率达61.69%、49.6%,各给药组与空白对照组比较差异均具有统计学意义(P<0.01),给药后小鼠体重及脏器指数与空白对照组比较稍低,但与阳性给药组比较对小鼠机体及免疫系统的损害小。结论:abrinP2在体内体外均有较强的抗肿瘤作用。

Effect of Abrin P2 on Anti tumor in Vitro and in Vivo

Objective：To study anti tumor activities of abrln P2 in vitro and in vivo. Methods：MTT assay was used to determine the inhibition effects of abrin P2 on proliferation of variety of cell lines in vitro; vivo anti - tumor test observed abrin P2 ＇s impact on the inhibition of tumors and the effect of body weight, thymus ,spleen index. Results： MTT showed that abrin P2 have a strong inhibitory rate on human nasopharyngeal carcinoma cell CNE -2Z, human lung cancer cell A -549, human oral epithelial cells KB,human gastric cancer cells BGC. In vivo oral administration of different doses of abrin P＇2 （0. 05mg/kg, 0. 075mg/kg and 0. 1 mg/kg）, a total of 10 days, It also have a strong inhibitory rate on Lewis, EMT6, Inhibition rate was 61.69% ,49. 6% ,the differences of treatment group and control group were statistically significant（ P 〈 0. 01 ）, body weight and organ index was slightly lower compared with the control group, but compared with the positive group the damage of body and the immune system is small, Conclusion： abrin P2 has a strong anti - tumor effect in vitro and in vivo.