| 萝藦果壳多糖脱蛋白方法研究 |
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| 引用本文： | 崔錾,韩冠英,马寅达,郭斌.萝藦果壳多糖脱蛋白方法研究[J].中国现代应用药学,2013,30(8):856-859. |
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| 作者姓名： | 崔錾 韩冠英 马寅达 郭斌 |
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| 作者单位： | 辽宁医学院，辽宁 锦州 121001;辽宁医学院附属第一医院，辽宁 锦州 121001;辽宁医学院附属第一医院，辽宁 锦州 121001;辽宁医学院附属第一医院，辽宁 锦州 121001 |
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| 摘 要： | 目的确定萝藦果壳多糖脱蛋白的最优方法。方法以蛋白脱除率和多糖损失率为指标,比较氯仿-正丁醇法(Sevag法)、三氯乙酸法(TCA法)、木瓜蛋白酶-Sevag法和木瓜蛋白酶-TCA法。结果 Sevag法、TCA法、木瓜蛋白酶-Sevag法、木瓜蛋白酶-TCA法的蛋白脱除率分别为73.14%,71.55%,86.03%,75.33%,多糖损失率分别为24.40%,25.68%,8.47%,17.13%。结论木瓜蛋白酶-Sevag法是除去萝藦果壳多糖中蛋白的最佳方法。
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| 关 键 词： | 萝藦果壳 多糖 脱蛋白 |
| 收稿时间： | 2013/1/21 0:00:00 |
| 修稿时间： | 2013/4/11 0:00:00 |
Study on Deproteinization Methods of Polysaccharide from Metaplexis Japonica Nutshell |
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| CUI Zan,HAN Guanying,MA Yinda and GUO Bin.Study on Deproteinization Methods of Polysaccharide from Metaplexis Japonica Nutshell[J].The Chinese Journal of Modern Applied Pharmacy,2013,30(8):856-859. |
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| Authors: | CUI Zan HAN Guanying MA Yinda and GUO Bin |
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| Affiliation: | Liaoning Medical University, Jinzhou 121001, China;The No.1 Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China;The No.1 Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China;The No.1 Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China |
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| Abstract: | OBJECTIVE To elect the best deproteinization method of polysaccharide from Metaplexis Japonica nutshell. METHODS The protein was removed by the methods of Sevag, TCA, papain-sevag and papain-TCA. The rate of deproteinization and the loss rate of polysaccharide were detected. RESULTS The percentage of deproteinization of Sevag, TCA, papain-Sevag and papain-TCA were 73.14%, 71.55%, 86.03%, 75.33% respectively and the loss rate of polysaccharide were 14.40%, 9.22%, 8.47%, 7.13% respectively. CONCLUSION The best deproteinization method of polysaccharide from Metaplexis Japonica nutshell was the papain-Sevag method. |
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| Keywords: | Metaplexis japonica nutshell polysaccharide deproteinization |
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