白术多糖脱蛋白脱色工艺

 目的：筛选白术多糖脱色、脱蛋白最佳工艺。方法：以脱蛋白率与多糖损失率为指标，选择最佳脱蛋白 方法；通过单因素和正交试验确定白术多糖最佳脱色条件。结果：三氯乙酸法（TCA）为最佳脱蛋白方法，白术多糖脱 色的最佳条件是：温度60 ℃，时间15 min，活性炭加入量为1%，pH值为5.5。结论：优化后的工艺条件稳定有效，可为 白术多糖工业化生产提供理论依据。     

一种简易的O_2与CO_2混合气体配制方法

一种简易的Ｏ_２与ＣＯ_２混合气体配制方法陈德森，付守芝，魏文芳，崔礼敬（郧阳医学院药理教研室十堰４４２０００）在科研及教学中所做的离体器官实验均需通９５％Ｏ２＋５％ＣＯ２或９７Ｏ２％＋３％ＣＯ２的混合气体，但这两种混合气体大多数地区不易购到，有介绍用...     

一种在多糖分离纯化过程中新的脱蛋白方法

目的　寻找一种在多糖 (香菇多糖 )分离纯化过程中适宜的脱蛋白方法。方法　以正交试验法为主。结果　通过正交试验法找到了一种在多糖 (香菇多糖 )分离纯化过程中新的脱蛋白方法。 5因素 3水平正交试验的结果表明 ,影响脱蛋白率 ( DPR)的主要因素是 B(正丁醇 )、次主要因素是 D(乙醇 ) ,而因素 C(甲醇 )和 E(氯化钠 )的影响则较小 ;影响 DPR的主次顺序为 B>D>A(氯仿 ) >E>C。 DPR随着正丁醇浓度的增加而变化很大 ,开始缓慢上升 ,然后迅速降低 ;随着乙醇浓度的增大 ,DPR逐渐降低 ;而当氯仿浓度增大时 ,DPR则开始上升 ,然后下降 ;当甲醇浓度增大时 ,DPR则变化很小。脱蛋白的最佳条件是 A2 B2 C1 D1 E2 ,即在氯仿 4 0 %、正丁醇 10 %、甲醇 2 %、乙醇4 0 %、氯化钠 0 .0 1%的条件下 ,杂蛋白的去除率最高。验证性试验结果表明 ,在此条件下 ,杂蛋白的去除率达99.98 %。通过反复试验 ,还找到了适用于工业化生产的包括香菇原料的蒸煮、煮沸样品的浓缩、粗多糖的沉淀和脱色、粗多糖的干燥等在内的原料预处理的最佳工艺。结论　这种新的脱蛋白方法具有工艺简单、无需贵重设备、加工成本低、容易放大等特点 ,特别适合于多糖类产品的精制或其他需要去除杂蛋白的单元操作     

Deproteinization of serum: another best approach to eliminate all forms of bilirubin interference on serum creatinine by the kinetic Jaffe reaction

The negative interference of conjugated, unconjugated, and delta bilirubin on patient serum creatinine determined by the kinetic Jaffe reaction is the unresolved problem. We compared bilirubin interference on thirty patients' serum creatinine obtained from four analyzers, with and without deprotenization before the Jaffe reaction, to the Vitros dry enzymatic method. We found significant negative interference from bilirubin on serum creatinine in all samples directly applied to four wet chemical methods, except the one incorporated with serum blank rate. The negative interferences linearly related to bilirubin concentration. However, bilirubin did not interfere on serum creatinine obtained from all wet chemical methods incorporated with deproteinization process before the reaction. We conclude that deproteinized serum before the reaction is the best approach to eliminate all forms of bilirubin interference on serum creatinine determined by the kinetic Jaffe reaction.     

响应面法优选铁皮石斛总多糖脱蛋白工艺

目的:优选铁皮石斛总多糖的胰蛋白酶联合Sevage法脱蛋白工艺,为该有效部位的深度开发和综合利用提供参考。方法:以总多糖保存率和蛋白脱除率为评价指标,通过单因素试验比较Sevage法、木瓜蛋白酶法、胰蛋白酶法和胰蛋白酶联合Sevage法;以蛋白脱除率为指标,酶解时间、酶用量、酶解温度和粗多糖溶液与Sevage试剂体积比为考察因素,利用BoxBehnken响应面法优化条件。结果:胰蛋白酶联合Sevage法具有较高的总多糖保存率及蛋白脱除率;最佳工艺条件为酶解时间1.5 h,酶用量0.21 g·m L-1,酶解温度63.25℃,总多糖溶液-Sevage试剂(4.27∶1);蛋白脱除率(87.15±7.93)%,总多糖保存率(81.32±8.54)%。结论:不同方法对铁皮石斛总多糖的脱蛋白效果有明显差异,胰蛋白酶联用Sevage法的效果最佳。     

萝藦果壳多糖脱蛋白方法研究

目的确定萝藦果壳多糖脱蛋白的最优方法。方法以蛋白脱除率和多糖损失率为指标,比较氯仿-正丁醇法(Sevag法)、三氯乙酸法(TCA法)、木瓜蛋白酶-Sevag法和木瓜蛋白酶-TCA法。结果 Sevag法、TCA法、木瓜蛋白酶-Sevag法、木瓜蛋白酶-TCA法的蛋白脱除率分别为73.14%,71.55%,86.03%,75.33%,多糖损失率分别为24.40%,25.68%,8.47%,17.13%。结论木瓜蛋白酶-Sevag法是除去萝藦果壳多糖中蛋白的最佳方法。     

Effect of smear layer deproteinization with HOCl solution on the dentin bonding of conventional and resin-modified glass-ionomer cements

The effect of smear layer-deproteinizing pretreatment using hypochlorous acid (HOCl) on the micro-shear bond strengths (μSBS) of conventional and resin-modified glass-ionomer cements (GIC) to dentin was investigated and compared with demineralizing pretreatment with polyacrylic acid (PAA). Three GICs: Fuji IX GP Extra (restorative conventional GIC), GC Fuji II LC EM (restorative resin-modified GIC), and GC Fuji Luting EX (luting resin-modified GIC), were used. One hundred fifty human molars were divided into groups (n = 10) according to the cements and dentin pretreatments; no pretreatment (control), 10 s PAA pretreatment, and HOCl pretreatment for 5, 15, or 30 s. After 24 h, μSBS was tested and the data were statistically analyzed using a two-way ANOVA, followed by Tukey's post-hoc test. HOCl pretreatment significantly increased μSBS of conventional GIC compared to the control group. For resin-modified restorative GIC, 5 s HOCl deproteinization significantly increased μSBS, while longer application times did not. There was no significant difference between HOCl-pretreated and control groups of resin-modified luting GIC. PAA pretreatment increased the μSBS of all cements significantly. In conclusion, smear layer deproteinization with HOCl can enhance the dentin bonding of conventional GIC. However, the residual radicals may adversely affect the polymerization of resin-modified GICs.     

枸杞多糖的精制工艺优选

目的:优化枸杞多糖(LBP)的精制工艺,为LBP的开发提供参考。方法:以多糖提取率为指标,采用正交试验考察提取次数、提取时间、料液比对多糖提取工艺的影响。在单因素试验的基础上,以糖损失率为指标,利用正交试验优化活性炭除色素工艺,以蛋白清除率和糖损失率的综合评分为指标,采用正交试验优化酶-sevag法脱蛋白工艺。结果:最佳提取工艺为提取次数2次、提取时间30 min、料液比1∶10。最佳除色素工艺为活性炭用量1.0%、温度100℃、时间20 min;糖损失率为28.6%。最佳除蛋白工艺为酶用量1.5%、温度50℃、时间60 min;蛋白质清除率为28.2%,糖损失率为10.9%。结论:优选的LBP精制工艺稳定可行,适用于LBP产品开发。     

大孔吸附树脂纯化野菊花多糖工艺

目的:研究大孔吸附树脂对野菊花多糖中所含色素和蛋白质的脱除性能.方法:比较LSA-700B,LSA-21,D101,XDA-8,AB-8,XDA-76种不同型号大孔吸附树脂对野菊花多糖的纯化效果；以脱色率、蛋白质去除率和多糖保留率作为考察指标,探讨温度、多糖质量浓度、pH、转速、流速5个因素对其纯化性能的影响.结果:LSA-21树脂对野菊花多糖的纯化效果较为理想；最佳工艺为温度40℃,多糖质量浓度7 g·L-1,pH 5,转速180 r·min-1,流速3 BV·h-1,径高比1∶8.在此条件下脱色率80.90％,蛋白质去除率52.84％,多糖保留率82.59％.结论:LSA-21大孔吸附树脂对野菊花多糖可以获得较高的纯化效率和多糖保留率.