用二阶导数光谱法测定栀子中总环烯醚萜苷的含量

目的：建立测定栀子中总环烯醚萜苷含量的二阶导数光谱法,并用该方法测定不同产地栀子中总环烯醚萜苷的含量。方法：采用二阶导数光谱法在263nm波长处测定栀子中总环烯醚萜苷的含量。结果：栀子苷在4.32～21.60μg/ml范围内呈良好线性关系;其回归方程为A=2.986×10-5c-1.833×10-5（r=0.9996）;回收率为（98.86±2.39）%（n=5）,精密度、重现性和稳定性良好。结论：本方法操作简单、准确,可用于测定栀子中总环烯醚萜苷的含量。

Determination of the content of total iridoid glycoside in Gardenia jasminoides Ellis by second order derivative spectrophotometry

Objective：To establish a second order derivative spectrophotometry method for determination of the content of total iridoid glycoside in Gardenia jasminoides Ellis from different regions.Methods：The second order derivative spectrophotometry was performed at the wavelength of 263 nm.Results：A good linear relationship was obtained in the range of 4.32-21.60 μg/ml of geniposide.The regression equation was A=2.986×10-5 c-1.833×10-5（r=0.999 6）.The average recovery rate was （98.86±2.39）% （n=5）.Conclusion：The method is simple and accurate,and can be used for determination of total iridoid glycoside in Gardenia jasminoides Ellis.