| 全反式维甲酸对人胃癌细胞株caveolin-1表达和定位的影响 |
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| 引用本文： | 施锐,张素梅,桂淑玉,周青,陈飞虎,汪渊.全反式维甲酸对人胃癌细胞株caveolin-1表达和定位的影响[J].安徽医科大学学报,2012,47(3):249-252. |
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| 作者姓名： | 施锐 张素梅 桂淑玉 周青 陈飞虎 汪渊 |
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| 作者单位： | 安徽医科大学分子生物学实验室、安徽省重要遗传疾病基因资源利用重点实验室,合肥,230032;安徽医科大学第一附属医院呼吸内科,合肥,230022;安徽医科大学药学院,合肥,230032 |
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| 基金项目： | 国家自然科学基金,安徽省自然科学基金 |
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| 摘 要： | 目的探讨全反式维甲酸(ATRA)对人胃癌细胞株SGC7901的增殖及对胞膜蛋白caveolin-1表达和定位的影响。方法 MTT法检测不同浓度(5、10、25μmol/L)ATRA对SGC7901细胞增殖的影响;免疫荧光法检测cavolin-1在细胞中的定位,Western blot检测caveolin-1的表达。结果 ATRA可明显抑制人胃癌细胞株SGC7901的增殖。ATRA处理3 d后,SGC7901细胞caveolin-1的表达无明显变化,但caveolin-1在胞浆中的分布明显降低,胞膜上分布明显增加。结论 ATRA可明显抑制SGC7901的增殖,促进caveolin-1在细胞膜上定位。
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| 关 键 词： | caveolin-1 全反式维甲酸 胃癌 |
The roles of ATRA on the expression and location of caveolin-1 in human gastric cancer cell line |
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| Affiliation: | Shi Rui1,Zhang Sumei1,Gui Shuyu2,et al(1Laboratory of Molecular Biology,Anhui Province and Key Laboratory of Gene Resource Utilization for Severe Disease Ministry of Education PRC,Hefei 230032; 2Dept of Respiratory Medicine,The First Affiliated Hospital of Anhui Medical University,Hefei 230022) |
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| Abstract: | Objective To study the influence of ATRA on proliferation of human gastric cancer cell lines SGC7901 expression and location of membrane protein caveolin-1 in vitro.Methods MTT method detected the influence of different concentration of ATRA(5,10,25 μmol/L) on proliferation of SGC7901.Immunofluorescence technique detected the expression and orientation of membrane protein cavolin-1 in cell.Western blot detected the expression of caveoin-1 in cytoplasm,membrane and total protein.Results ATRA could obviously inhibit human gastric cancer cell lines SGC7901 proliferation.Caveolin-1 which located in cell membrane increase,while the expression in cytoplasm did not change significantly after SGC7901 treated with ATRA three days.Conclusion ATRA can inhibit the progress of human gastric cancer by promoting the location of caveolin-1 in cell membranes. |
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| Keywords: | caveolin-1 ATRA gastric cancer |
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