赤灵芝孢子粉水溶性提取物(肌生注射液)对小鼠的催眠镇静作用

目的：研究赤灵芝孢子粉水提物（肌生注射液）对小鼠中枢神经系统的作用。方法：以翻正反射消失出现时间作为睡眠时间,观察不同剂量和给药方式下肌生液对小鼠戊巴比妥钠和巴比妥钠睡眠时间及自主活动等的影响。结果：皮下注射肌生液可明显延长小鼠戊巴比妥钠和巴比妥钠睡眠时间，且有剂量效应关系。肌生液亦可诱导注射阈下剂量的戊巴比妥的小鼠入睡。每日注射具有镇静作用剂量的肌生液一次连续8天不产生依赖性，而安定连续给药8天后产生依赖性。肌生液可明显减少小鼠的自主活动。结论：灵芝孢子粉水提物对小鼠中枢神经系统具有镇静催眠的效果。

Study on the hypnotic and sedative effect of Jisheng Injection

Objective: To study the hypnotic and sedative effects of Jisheng Injection on the central nervous system in mice. Methods: The interval of disappearance and recovery of the righting reflex of mice was recorded as sleeping time to evaluate the effect of Jisheng on sodium pentobarbital and barbital sleeping time or spontaneous motor activity with different doses and administration manners. Results: Subcutaneous injection of Jisheng extract significantly prolonged the sleeping time of both sodium pentobarbital and barbital in mice in a dose-dependent manner and also induced mouse to sleep after intraperitoneal injection of a subthreshold dose of pentobarbital which could not induce sleep in nomal mice. Repeated injection of the effectively hypnotic dosage of Jisheng extract once daily for consecutive 8 days did not induce addiction, as the prolonging effect of Jisheng on pentobarbital sleeping time was not shortened. Whereas the diazepam induced addiction after repeated injection for 8 days. Jisheng injection markedly decreased the spontaneous motor activity in mice. Conclusion: The aqueous extract of G. lucidum spores has hypnotic and sedative actions on the central nervous system in mice.