葛根异黄酮对高尿酸血症小鼠炎症、应激反应的影响研究

背景 人类因缺乏尿酸酶基因而无法代谢不溶性尿酸(UA),而高嘌呤饮食、代谢疾病均会导致UA升高,病情严重者可诱发肾功能不全等疾病.黄酮类化合物对多种酶有抑制作用,异黄酮是中药葛根的主要功效成分,其已被证实具有明确的降糖、降脂作用.目的 探讨葛根异黄酮对高尿酸血症(HUA)小鼠炎症、应激反应的影响.方法 2020年12月...

Effect of Pueraria Isoflavones on Inflammation and Stress Response in Hyperuricemia Mice

Background Humans cannot metabolize insoluble uric acid(UA)due to the lack of uricase gene,and high purine diet and metabolic diseases can lead to increased UA,and severe cases can induce diseases such as renal insufficiency.Flavonoids have inhibitory effects on a variety of enzymes,isoflavones are the main functional components of traditional Chinese medicine Pueraria,which have been confirmed to have clear hypoglycemic and lipid-lowering effects.Objective To investigate the effect of pueraria isoflavones on inflammation and stress response in hyperuricemia(HUA)mice.Methods From December 2020 to June 2021,48 SPF Kunming mice were divided into normal group,model group,condition control group,and experimental group by random number table method,with 12 mice in each group.The mice in the normal group were given 0.5%sodium carboxymethyl cellulose by gavage every day;the mice in the other three groups were given an equal volume of a mixture of adenine and ethambutol hydrochloride by gavage every day to make the HUA model,for a total of 30 d.After successful modeling,the mice in the normal group were fed with mixed feed without other drugs;the mice in the model group were continuously gavaged with 0.5%carboxymethyl cellulose sodium without other drugs;the mice in the condition control group were continuously gavaged with 0.5%carboxymethyl cellulose sodium and fibrate solution at the same time;the mice in the experimental group were gavaged with 0.5%carboxymethyl cellulose sodium and pueraria isoflavones at the same time;the intervention time of the four groups was 30 d.Serum UA,inflammatory indexesincluding substance P(SP),erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),tumor necrosis factor alpha(TNF-α),interleukin 6(IL-6)],and stress response indexesincluding superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),malondialdehyde(MDA),nitric oxide(NO),nitric oxide synthase(NOS)]before and after intervention in four groups were compared.The pathological characteristics of aortic arch endothelial cells in four groups were observed.Results After intervention,the serum UA of the mice in the model group,condition control group and experimental group was higher than that of the normal group,and the serum UA of the mice in the condition control group and experimental group was lower than that of the model group(P<0.05);the serum UA of mice in the model group,condition control group and experimental group after intervention was higher than that before intervention,respectively(P<0.05).After intervention,the serum SP,ESR,CRP and TNF-α of the mice in the model group,conditional control group and experimental group were higher than those in the normal group,the serum SP,ESR,CRP and TNF-α of the mice in the condition control group and experimental group were lower than those in the model group,and the serum SP,ESR and CRP of the mice in the experimental group were lower than those in the condition control group(P<0.05);the serum SP,ESR,CRP and TNF-α of mice in the model group,condition control group and experimental group after intervention were higher than those before intervention,respectively(P<0.05).After intervention,the serum SOD of the mice in the model group,condition control group and experimental group was higher than that of the normal group,and the serum GSH-Px of the mice was lower than that of the normal group(P<0.05);after intervention,the serum MDA of the mice in the model group and condition control group was higher than that in the normal group(P<0.05);after intervention,the serum SOD and MDA of the mice in the condition control group and experimental group were lower than those in the model group,and serum GSH-Px was higher than that in the model group(P<0.05);after intervention,the serum SOD and MDA of the mice in the experimental group were lower than those in the conditional control group,and the serum GSH-Px was higher than that in the conditional control group(P<0.05).The serum SOD and MDA of mice in the model group,condition control group and experimental group after intervention were higher than those before intervention,and serum GSH-Px were lower than those before intervention,respectively(P<0.05).The endothelial cells of the aortic arch of mice in the normal group were arranged regularly without obvious cracks.The endothelial cells of the aortic arch of mice in the model group had obvious watery degeneration,the cytoplasm and nucleus were slightly swollen,and there were lymphocytes in some areas.Some aortic arch endothelial cells in the condition control group and experimental group had binucleated cells,and the endothelial cells in the experimental group recovered well and the cell morphology was relatively complete.Conclusion Compared with febux solution,pueraria isoflavones can more effectively reduce disease severity in HUA mice while attenuating inflammatory and stress responses.