| 143例HIV初筛阳性标本免疫印迹试验结果分析 |
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| 引用本文： | 崔为国,王哲,梁振山,李宏,薛晓玲,朱新朋,孙国清,李洁.143例HIV初筛阳性标本免疫印迹试验结果分析[J].实用预防医学,2003,10(6):858-859. |
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| 作者姓名： | 崔为国 王哲 梁振山 李宏 薛晓玲 朱新朋 孙国清 李洁 |
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| 作者单位： | 1. 河南省疾病预防控制中心,中国河南,郑州,450003
2. 中国人民解放军信息工程大学卫生处 |
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| 摘 要： | 目的 使用酶联免疫吸附法(ELISA)、硒标免疫层析法(Determine)及免疫印迹法(WB)对143例血清标本做初筛及确认试验。方法 操作及结果判断按照《全国艾滋病检测技术规范》施行。结果 143例初筛试验阳性经免疫印迹试验确认141例HIV—1阳性，且条带出现百分率gp^120、p^66为100％，gp^160、gp^41、p^51、p^34、p^55为90％以上，1例HIV—1合并gp^36出现。结论 初筛结果有假阳性须经确认试验才能得出准确结果。
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| 关 键 词： | 人类免疫缺陷病毒 酶联免疫吸附试验 硒标免疫层析试验 免疫印迹试验 |
| 文章编号： | 1006-3110(2003)06-0858-02 |
| 修稿时间： | 2003年8月15日 |
Western Blot Assay To 143 HIV Positive Specimens of Screening Test |
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| CUI Wei guo,WANG Zhe,LIANG Zhen shan,et al..Western Blot Assay To 143 HIV Positive Specimens of Screening Test[J].Practical Preventive Medicine,2003,10(6):858-859. |
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| Authors: | CUI Wei guo WANG Zhe LIANG Zhen shan |
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| Abstract: | Objective To study the role of ELISA , Determine and Western Blot Test in screening test and confirmation test on 143 serum specimens. Methods Operations and results were determined by National AIDS/HIV Test Technology Regulation. Result 141 HIV-1 positive specimens were confirmed by WB Test among 143 screening test positive specimens. There were gp 120 , p 66 reactive bands(100%),gp 160 ,gp 41 ,p 51 ,p 34 ,p 55 reactive bands (>90%), only 1 HIV-1 with gp36 reactive band among 141 specimens. Conclusion There were false positive results in the screening test and the results must be determined by confirmation test. |
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| Keywords: | HIV ELISA Determine Western Blot Test |
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