基因芯片分析孤儿核受体NURR1上调表达对前列腺癌LNCaP细胞基因表达谱的影响

目的探讨孤儿核受体NURR1表达上调对前列腺癌细胞基因表达谱的影响。 方法构建NURR1过表达载体，通过慢病毒感染方式上调前列腺癌LNCaP细胞中NURR1的表达水平。通过免疫细胞化学染色检测NURR1蛋白的表达，通过基因表达谱芯片分析NURR1过表达组和对照组LNCaP细胞的mRNA、lncRNA表达水平的变化。通过KEEG和GO分析探讨NURR1过表达对相关信号通路和功能的影响。 结果NURR1基因在LNCaP细胞过表达，与对照组比较共有515个基因发生差异表达，其中上调的mRNA238个，下调的mRNA195个；上调的lncRNA54个，下调的lncRNA28个。差异表达的mRNA和lncRNA主要在于细胞分子功能的改变，其中视网膜结合功能、铁离子结合能力、神经轴突调控以及氧化还原过程、细胞外区域的细胞学组成改变最为显著。细胞因子与细胞因子受体的相互作用以及调节干细胞多能性的有关信号通路都发生显著性改变。 结论NURR1基因的上调表达对前列腺癌细胞mRNA以及lncRNA表达谱显著改变，可能与其促进前列腺癌进展的功能有关。

Profiling study of NURR1-overexpressed LNCaP cells by gene chip analysis

ObjectiveTo investigate the profiling of NURR1-overexpressed LNCaP cells by gene chip analysis. MethodsNURR1 was ectopic expressed in LNCaP cells by lentivirus infection, validated by immunocytochemical staining. The profiling of mRNA and lncRNA was compared by gene chips between NURR1-overexpressed LNCaP cells and vector-transfected control. KEEG and GO analysis were performed to study the potential functional role and signaling pathways involved. ResultsA total of 515 genes were differentially expressed in NURR1-overexpressed LNCaP cells as compared to its vector-transfected control, including 238 mRNA upregulated, 195 mRNA down regulated, 54 lncRNA upregulated and 28 lncRNA downregulated. The differentially expressed mRNA and lncRNA were mainly involved in molecular functions, among which the retinal binding function, iron ion binding capacity, nerve axon regulation and redox process, and the cytological composition of the extracellular region exhibited the most significance. The interaction between cytokines and cytokine receptors, as well as stem cell pluripotency related signaling pathways were changed significantly. ConclusionThe mRNA and lncRNA profiling has been significantly changed by the ectopic expression of NURR1, which might play critical role in the development progression of prostate cancer.