核磁共振波谱法测定臭氧化橄榄油中的活性成分含量

以橄榄油为原料,经臭氧氧化制备臭氧化橄榄油。利用核磁共振(NMR)采集碳谱数据,积分碳谱特征峰,计算橄榄油被氧化成分的相对含量。结果表明:臭氧化橄榄油与橄榄油碳谱有明显的区别,臭氧化橄榄油在δ103.1处出现新峰,对应δ130.1处的峰高减弱,这是由于橄榄油中的不饱和双键被氧化。采用该方法测得5个臭氧化油中活性成分含量分别为21.46%、21.03%、21.31%、18.71%和24.42%。采用核磁共振波谱法测定臭氧化橄榄油的活性成分含量具有操作简单,不需要预处理,结果可靠的优点。

Determination of active components content in ozonized olive oil by NMR

The ozonized products were prepared from olive oil by ozonation reaction. The data of carbon spectrum was collected by NMR,and the characteristic peaks of 13C NMR were integrated to calculate the relative content of oxidized components in olive oil. The results showed that the carbon spectrum of olive oil had significant differences from the ozonized olive oil. The new peak appeared at δ 103. 1,corresponding to the weakening of the peak height at δ 130. 1,which was due to the oxidation of unsaturated double bonds in olive oil. The active component contents in five ozonized oils determined by the method were 21. 46%,21. 03%,21. 31%,18. 71% and 24. 42%,respectively. The method of determining the active component content in ozonized olive oil by NMR had the advantages of simple operation,no pretreatment and reliable results.