| 亚油酸氢过氧化物氧化修饰对大豆蛋白热变性和聚集的影响 |
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| 引用本文： | 吴伟,林亲录,华欲飞.亚油酸氢过氧化物氧化修饰对大豆蛋白热变性和聚集的影响[J].中国油脂,2011,36(5). |
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| 作者姓名： | 吴伟 林亲录 华欲飞 |
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| 作者单位： | 1. 中南林业科技大学,食品科学与工程学院,长沙,410004;粮油深加工与品质控制湖南省重点实验室,长沙,410004
2. 江南大学食品学院,江苏,无锡,214122 |
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| 基金项目： | 国家自然科学基金，食品科学与技术国家重点实验室目标导向课题 |
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| 摘 要： | 以亚油酸氢过氧化物代表脂质氢过氧化物氧化修饰大豆分离蛋白,采用圆二色光谱、内源荧光光谱、粒径分析以及相对分子质量分布研究氢过氧化物氧化修饰对大豆蛋白热变性和聚集的影响.结果发现亚油酸氢过氧化物氧化修饰使得大豆蛋白热稳定性下降,热变性过程中形成聚集体粒径随着蛋白质氧化程度的升高呈现先增加后减小的趋势,热变性大豆蛋白冷却后的粒径和聚集体含量则随着蛋白质氧化程度的增加而下降.
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| 关 键 词： | 亚油酸氢过氧化物 大豆蛋白 聚集 |
Effects of oxidative modification by linoleic acid hydroperoxide on the thermal denaturation and aggregation of soy protein |
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| WU Wei,LIN Qinlu,HUA Yufei.Effects of oxidative modification by linoleic acid hydroperoxide on the thermal denaturation and aggregation of soy protein[J].China Oils and Fats,2011,36(5). |
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| Authors: | WU Wei LIN Qinlu HUA Yufei |
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| Affiliation: | WU Wei1,2,LIN Qinlu1,HUA Yufei3(1.School of Food Science and Engineering,Central South University of Forestry and Technology,Changsha 410004,China,2.Hunan Key Laboratory of Grain-oil Process and Quality Control,3.School of Food Science and Technology,Jiangnan University,Wuxi 214122,Jiangsu,China) |
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| Abstract: | Linoleic acid hydroperoxide was selected as a representative of lipid hydroperoxide to investigate the effects of oxidative modification by lipid hydroperoxide on the thermal denaturation and aggregation of soy protein by circular dichroism,intrinsic fluorescence,particle size analysis and relative molecular weight distribution.The results revealed that oxidative modification by linoleic acid hydroperoxide resulted in a decrease of thermal stability of soy protein.As the extent of soy protein oxidation incr... |
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| Keywords: | linoleic acid hydroperoxide soy protein aggregation |
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