富硒碎米荠不同提取物抗氧化性能研究

为进一步开发利用恩施堇叶碎米荠,通过比较测定3种碎米荠提取物:碎米荠碱提物(CE)、碎米荠富硒蛋白(SPR)和碎米荠富硒多肽(SPI)对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、羟自由基(OH·)、2'-联氨-双-3-乙基苯并噻唑啉-6-磺酸自由基(ABTS+·)和超氧阴离子自由基(O_2~-·)的清除能力,考察3种富硒碎米荠提取物的体外抗氧化性能,并与富硒酵母(SY)、无机硒(IS)和非富硒多肽与无机硒混合物(SPIS)进行比较。结果表明:SPI清除4种自由基能力最强,其对DPPH·、OH·、ABTS~+·和O_2~-·的清除率分别为87.5%,50.3%,99.7%,45.7%,IS清除4种自由基能力最弱,均不超过14%。通过比较SPR、SPI、CE、SY、IS和SPIS对4种自由基的清除率及半抑制浓度(IC_(50)),发现有机硒清除自由基的能力强于无机硒。

Study on antioxidant capacity of selenium-substance form cardamine

The 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH·), hydroxyl free radical (OH·), 2, 2''-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) free radical (ABTS⁺·) and superoxide radical (O^-₂·) scavenging ability were selected as the four indicators to evaluate the in vitro antioxidant properties of different selenium-containing substances, and compared with selenium-enriched yeast (SY) and non-selenium-enriched peptides and inorganic selenium mixture (SPIS).The results showed that SPI had the strongest ability to scavenge four kinds of free radicals, and its DPPH·, OH·, ABTS⁺· and O^-₂· scavenging rates were 87.5%, 50.3%, 99.7% and 45.7%. The weakest sample was IS, and its radicals scavenging rates was no more than 14%. By comparing the scavenging rates and semi-inhibitory concentration (IC₅₀) of four radicals of SPR, SPI, CE, SY, IS and SPIS, it was found that the ability to scavenge free radicals of organic selenium was stronger than inorganic selenium.