| 流动分析法测定烟草中的纤维素 |
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| 引用本文： | 尚军,吕祥敏,王鹏,何蓉,文鹏,周维.流动分析法测定烟草中的纤维素[J].烟草科技,2012(7):40-42. |
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| 作者姓名： | 尚军 吕祥敏 王鹏 何蓉 文鹏 周维 |
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| 作者单位： | 1. 重庆烟草工业有限责任公司,重庆市南岸区南坪东路2号17楼,400060
2. 重庆烟草工业有限责任公司,重庆市南岸区南坪东路2号17楼 400060;川渝中烟工业公司,成都市锦江区成龙大道一段56号 610066 |
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| 摘 要： | 建立了测定烟草中纤维素含量的流动分析法。采用纤维素酶酶解烟草中的纤维素,利用流动分析仪测定水解液中的葡萄糖。结果表明:①最优酶解条件为:烟粉0.25 g,缓冲溶液60 mL,酶用量0.25 g,水解温度37℃,水解时间24 h;②方法的相对标准偏差(RSD)为2.40%,回收率为96.7%～103.8%,检测限为0.11 mg/mL。该方法适合于烟草中纤维素含量的测定。
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| 关 键 词： | 流动分析法 烟草 纤维素 酶解 |
Determination of Cellulose in Tobacco with Flow Analyzer |
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| SHANG Jun
,
L Xiangmin
,
WANG Peng
,
HE Rong
,
WEN Peng
,
ZHOU Wei.Determination of Cellulose in Tobacco with Flow Analyzer[J].Tobacco Science & Technology,2012(7):40-42. |
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| Authors: | SHANG Jun L Xiangmin WANG Peng HE Rong WEN Peng ZHOU Wei |
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| Affiliation: | SHANG Jun
,
L(U) Xiangmin
,
WANG Peng
,
HE Rong
,
WEN Peng
,
ZHOU Wei |
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| Abstract: | A flow analysis method for determining the cellulose in tobacco was developed.Cellulose was hydrolyzed by cellulase and the formed glucose was determined by flow analyzer.The results showed that:1) The optimal hydrolysis conditions were:tobacco powder 0.25 g,buffer solution 60 mL,enzyme 0.25 g,hydrolysis temperature 37 ℃,hydrolysis time 24 hours.2)The relative standard deviation(RSD) of the method was 2.40% with the recoveries from 96.7% to 103.8% and the limit of detection of 0.11 mg/mL.This method is suitable for determining the content of cellulose in tobacco. |
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| Keywords: | Flow analyzer Tobacco Cellulose Enzymatic hydrolysis |
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