Transient viscoelasticity study of tobacco mosaic virus/Ba2+ superlattice

Recently, we reported a new method to synthesize the rod-like tobacco mosaic virus (TMV) superlattice. To explore its potentials in nanolattice templating and tissue scaffolding, this work focused the viscoelasticity of the superlattice with a novel transient method via atomic force microscopy (AFM). For measuring viscoelasticity, in contrast to previous methods that assessed the oscillating response, the method proposed in this work enabled us to determine the transient response (creep or relaxation) of micro/nanobiomaterials. The mathematical model and numerical process were elaborated to extract the viscoelastic properties from the indentation data. The adhesion between the AFM tip and the sample was included in the indentation model. Through the functional equation method, the elastic solution for the indentation model was extended to the viscoelastic solution so that the time dependent force vs. displacement relation could be attained. To simplify the solving of the differential equation, a standard solid model was modified to obtain the elastic and viscoelastic components of the sample. The viscoelastic responses with different mechanical stimuli and the dynamic properties were also investigated.     

氢氧化钠/尿素法和硫酸法测定烟草木质素的对比分析

为了对比氢氧化钠/尿素法(NU法)和硫酸法在测定烟草中木质素含量(质量分数,下同)的差异性,采用NU法和硫酸法测定广东、四川、湖南、云南和贵州5个产区烟梗和烟叶的木质素含量,分析了两种方法在预处理过程的干扰物质去除率及酸解过程木质素含量和结构变化。结果表明:1与硫酸法相比,NU法预处理对干扰物质的去除率更高。2NU法采用稀酸酸解,减少了烟草中木质素的降解;选择在325 nm处测定酸溶木质素(ASL)的紫外吸光度,避免了蛋白质和糠醛对ASL的干扰,提高了烟草木质素测定结果的准确度和重现性。3与硫酸法相比,NU法分离得到的酸不溶木质素(AIL)的氧化程度低、结构变化小,因而更能反映烟草木质素的结构特征。NU法比硫酸法更加适用于烟草木质素的含量测定。     

一种实现企业内部员工高效协同的信息化建设思路

本文以四川省烟草专卖局（公司）效能建设的信息化支撑———效能协同办公平台的建设为背景,围绕企业内部员工之间的沟通与协作、办公效率等所面临的一系列问题进行思考,从企业发展面临问题、信息系统建设现状,未来信息化一体化目标等方面进行综合分析,并结合当前业界主流的信息技术应用方向,提出了一种面向企业内部员工的高效协同的建设思路,期望借以此文为企业通过信息化手段降低员工日常工作复杂度、提高员工工作效率、增强企业自身竞争力提供借鉴。     

MBBR工艺在烟草行业污水处理的应用

对MBBR工艺原理、工艺特点以及应用状况进行了介绍,对烟草行业的污水来源及水质状况进行了分析,重点就运用MBBR工艺及设备处理卷烟厂的污水工艺流程进行了论述,提出了在烟草行业的应用前景。     

基于ANSYS Workbench的烟包机推包机构模态分析

烟包机是包装行业中重要的包装机器之一,随着们对香烟需求量的增加,香烟生产厂对烟包机的速度要求越来越高。由于振动会造成结构的共振或疲劳,从而破坏结构,所以针对烟包机推包机构高速运动时容易产生振动的问题进行研究,分析推包机构主要部件平面沟槽凸轮,利用Pro/E建立平面沟槽凸轮实体模型,然后导入Workbench软件中进行自由模态分析和约束模态分析,求出其振动特性,为推包机的优化设计提供了必要依据。     

Anti-TMV Activity of Malformin A1, a Cyclic Penta-Peptide Produced by an Endophytic Fungus Aspergillus tubingensis FJBJ11

Plant-associated microorganisms are known to produce a variety of metabolites with novel structures and interesting biological activities. An endophytic fungus FJBJ11, isolated from the plant tissue of Brucea javanica (L.) Merr. (Simaroubaceae), was proven to be significantly effective in producing metabolites with anti-Tobacco mosaic virus (TMV) activities. The isolate was identified as Aspergillus tubingensis FJBJ11 based on morphological characteristics and ITS sequence. Bioassay-guided isolation led to the identification of a cycli penta-peptide, malformin A₁, along with two cyclic dipeptides, cyclo (Gly-l-Pro) and cyclo (Ala-Leu). Malformin A₁ showed potent inhibitory effect against the infection and replication of TMV with IC₅₀ values of 19.7 and 45.4 μg·mL⁻¹, as tested using local lesion assay and leaf-disc method, respectively. The results indicated the potential use of malformin A₁ as a leading compound or a promising candidate of new viricide.     

《烟草控制框架公约》的法律适用浅析

从国际条约的性质及在我国国内法上的适用为出发点,对《烟草控制框架公约》及其实施指南的法律地位和适用原则进行分析,试图为《公约》及其实施指南在中国的法律适用和具体实践提供法理依据.     

Catalytic coating of virus particles with zinc oxide

We show that the rod-shaped Tobacco mosaic virus (TMV) can be coated with the wide band gap semiconductor zinc oxide by electroless deposition under mild reaction conditions. The process involves pretreatment with aqueous Pd(II), followed by nucleation of ZnO, directed to palladium centers formed on the surface of TMV. The mechanism is based on the local pH change due to the reduction of nitrate by dimethylamine borane at the palladium. Morphology and chemical composition have been analyzed by transmission electron microscopy (TEM) and by energy filtering TEM, respectively. The optical properties were elucidated by cathodoluminescence, which showed the desired near-band edge emission.     

提取叶蛋白质所用烟草的栽培技术试验初报

以烟草鲜叶为原料提取叶蛋白质，对种植密度，施用氮肥和采叶方法３项栽培技术进行了试验结果提示，在土壤气候适宜条件下，可按２１００００株／ｈｍ＾２的密度种植，在施肥的基础上，应按２２５ｋｇ／ｈｍ＾２增施搂肥，每次采叶后按１５０ｋｇ／ｈｍ＾２追施氮肥；采叶方法可根据具体情况选用砍株留茬法或摘叶留顶法，或二者交替使用。     

烟草香味成分的超高压萃取

为了快速高效地萃取烟草香味成分,进行了烟草香味成分的超高压萃取(UHPE)试验,并利用响应面分析法对萃取参数进行了优化。结果表明:烟草香味成分的最优UHPE条件为萃取压力380MPa、保压时间20min、萃取溶剂75%乙醇,液料比15,在此条件下,香味成分萃取总量理论值180.9μg/g,验证值179.8μg/g。