| 油菜薹和蕾中硫代葡萄糖苷组分及含量分析 |
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| 引用本文： | 彭,君,胡,阳,龚,照,林处发,高长斌,汪爱华.油菜薹和蕾中硫代葡萄糖苷组分及含量分析[J].中国蔬菜,2019,1(3):30-36. |
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| 作者姓名： | 彭 君 胡 阳 龚 照 林处发 高长斌 汪爱华 |
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| 作者单位： | （;1. 华中农业大学园艺林学学院，湖北武汉 430070；;2.武汉市农业科学院，湖北武汉 430070；;3.长江大学园艺园林学院，湖北荆州 434025；;4.荆州农业科学院，湖北荆州 434000） |
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| 基金项目： | 七大农作物育种专项（2016YFD0100202-28） |
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| 摘 要： | 采用超高效液相色谱（UPLC）法，对30 个油菜品种薹和蕾中硫代葡萄糖苷（glucosinolate，简称硫苷）的组分及含量进行测定。结果表明：30 个油菜品种的总硫苷含量变异范围较大，在21.67～119.29 μmol·g-1 之间；大多数油菜品种蕾中的硫苷含量高于薹，蕾中硫苷含量均值为37.26 μmol·g-1，薹中为28.06 μmol·g-1；薹和蕾中的硫苷组分相似，均以4- 戊烯基硫苷和2- 羟基-3- 丁烯基硫苷为主要成分，两者在薹和蕾中的含量分别占总硫苷含量的46.24%、28.52% 和45.19%、29.13%。进一步分析高、中、低硫苷含量油菜品种的硫苷组分及含量，结果表明油菜不同品种间硫苷含量的差异主要是由4- 戊烯基硫苷引起的。
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| 关 键 词： | 油菜 薹 蕾 硫代葡萄糖苷 超高效液相色谱（UPLC） |
Component and Content Analysis of Glucosinolates in Rapeseed Flowering Stalk and Bud |
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| PENG Jun,HU Yang,GONG Zhao,LIN Chu-fa,GAO Chang-bin,WANG Ai-hua.Component and Content Analysis of Glucosinolates in Rapeseed Flowering Stalk and Bud[J].China Vegetables,2019,1(3):30-36. |
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| Authors: | PENG Jun HU Yang GONG Zhao LIN Chu-fa GAO Chang-bin WANG Ai-hua |
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| Affiliation: | （1College of Horticulture and Forestry，Huazhong Agriculture University，Wuhan 430070，Hubei，China；2Wuhan;
Academy of Agricultural Sciences，Wuhan 430070，Hubei，China；3College of Horticulture and Gardening，Yangtze;
University，Jingzhou 434025，Hubei，China；4Jingzhou Institute of Agricultural Sciences，Jingzhou 434000，Hubei，;
China） |
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| Abstract: | In this study，the glucosinolate（GS）component and content in flower stalk and bud from 30
rapeseed varieties were measured by ultra-high performance liquid chromatography（UPLC）．The results showed
that there were bigger variation ranges in the total glucosinolates contents of these 30 rapeseed varieties，between
21.67-119.29 μmol·g-1．The glucosinolate contents in bud were mostly higher than that in flowering stalk．The
average glucosinolate content in bud was 37.26 μmol·g-1，while that in flowering stalk was 28.06 μmol·g-1．
The glucosinolate components in bud and flowering stalk were similar，all took 4-pentenyl glucosinolate and
2-hydroxy-3-butenyl glucosinolate as their basis．Their contents in bud and flowering stalk accounted for
46.24%，28.52% and 45.19%，29.13% of the total glucosinolates contents，respectively．Further analysis
was conducted in the glucosinolate components and contents of rapeseed varieties with high，medium and low
glucosinolate contents．The results showed that the differences in glucosinolate contents were mainly caused by
4-pentenyl glucosinolate． |
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| Keywords: | Rapeseed Flowering stalk Bud Glucosinolate Ultra-high performance liquid chromatography（UPLC） |
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