| 胆碱酯酶生物传感器测定有机磷农药敌敌畏 |
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| 引用本文： | 魏福祥,韩菊,刘庆洲,王金梅.胆碱酯酶生物传感器测定有机磷农药敌敌畏[J].河北科技大学学报,2003,24(4):92-94. |
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| 作者姓名： | 魏福祥 韩菊 刘庆洲 王金梅 |
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| 作者单位： | 河北科技大学环境科学与工程学院,河北石家庄,050018 |
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| 基金项目： | 国家"863"计划课题(2001AA635130) |
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| 摘 要： | 以固定化乙酰胆碱酯酶作敏感物质、银基汞膜电极作基础电极,制备了安培型生物传感器。采用单扫描伏安法考察了传感器对有机磷农药敌敌畏的响应特性。以乙酰硫代胆碱作底物,在0.05mol/LpH=7.0的硼砂缓冲溶液中,底物水解产物硫代胆碱在-0.33V处有灵敏的还原峰。在1.0×10-8～1.0×10-6mol/L浓度范围内对敌敌畏进行了测定,校准曲线呈现良好的线性关系,r=0.9988。抑制率为5%时,对敌敌畏的检出限为2.8×10-10mol/L。
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| 关 键 词： | 乙酰胆碱酯酶 生物传感器 有机磷农药 敌敌畏 |
| 文章编号： | 1008-1542(2003)04-0092-03 |
| 修稿时间： | 2003年6月11日 |
Determination of Dichlorvos by a Acetylcholinesterase Biosensor |
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| WEI Fu-xiang,HAN Ju,LIU Qing-zhou,WANG Jin-mei.Determination of Dichlorvos by a Acetylcholinesterase Biosensor[J].Journal of Hebei University of Science and Technology,2003,24(4):92-94. |
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| Authors: | WEI Fu-xiang HAN Ju LIU Qing-zhou WANG Jin-mei |
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| Abstract: | A acetylcholinesterase(AChE) amperomerometric biosensor is prepared for determination of organophosphorus insecticide, such as dichlorvos. The response characteristic of the AChE biosensor is studied by linear sweep voltammetry. With acetylthiocholine as substrate, in 0.05 mol/L borax buffer solution(pH=7.0), a sensitive voltammetric reduction peak of thiocholine formed by acetylthiocholine hydrolysis is observed. The peak potential is -0.33 V(vs.SCE). The calibration graphs are linear from 1.0×10~(-8) to 1.0×10~(-6) mol/L for dichlorvos. The detection limit (5% of inhibition) for dichlorvos is found to be 2.8×10~(-10) mol/L. |
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| Keywords: | acetylcholinesterase biosensor organophosphorus insecticide dichlorvos |
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