FT-IR和HPLC对不同产地大黄药材的鉴别分析

目的 采用傅里叶变换红外光谱（FT-IR）和高效液相色谱（HPLC）方法对37批不同品种和产地的大黄药材进行分析。方法 分别采用FT-IR和HPLC测量37批大黄药材的红外光谱和液相指纹图谱，并对结果进行聚类分析。从每一产地中选择一批药材，分析红外原始光谱，并对原始光谱进行二阶导数处理，根据所得二阶导数光谱对各产地大黄进行分析；同时对HPLC指纹图谱进行相似度分析。结果 FT-IR和HPLC两种方法聚类分析结果基本一致。通过分析不同产地的大黄药材原始红外光谱特征峰能够区分各产地大黄品种，但无法区分其产地，而二阶导数特征峰有明显的位置、形态和强度上的差异，能够将不同产地大黄药材鉴别出来。HPLC指纹图谱相似度分析无法将其产地进行区分。结论 与HPLC指纹图谱相比，FT-IR能够更加全面的反映大黄药材的化学成分，并对其品种和产地进行区分。

IDENTIFICATION AND ANALYSIS OF RHUBARB MEDICINAL MATERIALS FROM DIFFERENT HABITATS BY FT-IR AND HPLC

Objective: To analyze 37 batches of rhubarb medicinal materials of different varieties and origins. Methods: FT-IR and HPLC methods were used to measure the infrared spectrum and Liquid phase fingerprint of 37 batches of rhubarb, and cluster analysis were conducted. A batch of medicinal materials from each place of origin was selected for further analysis, the original infrared spectrum was analyzed and processed with the second derivative of the original spectrum. According to the obtained second derivative spectrum, the rhubarb of each origin was analyzed; the similarity of the HPLC fingerprint was also analyzed. Results: The results of cluster analysis by FT-IR and HPLC were basically consistent. The varieties of rhubarb from different regions could be distinguished, but their origins could not be distinguished. The second derivative characteristic peaks had obvious differences in position, shape and intensity, which could be used to distinguish rhubarb medicinal materials from different origins. The origins could not be distinguished by HPLC fingerprint similarity analysis. Conclusion: Compared with HPLC fingerprint, FT-IR can reflect the chemical composition of rhubarb more comprehensively, and distinguish the varieties and origins.