| 用酵母双杂合系统筛选与人血小板生成素受体c-Mpl相互作用的蛋白质的初报 |
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| 引用本文： | 周伟国,冯丽冰,赵新燕,戴卫列,李昌本,赵寿元.用酵母双杂合系统筛选与人血小板生成素受体c-Mpl相互作用的蛋白质的初报[J].复旦学报(自然科学版),1999(5). |
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| 作者姓名： | 周伟国 冯丽冰 赵新燕 戴卫列 李昌本 赵寿元 |
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| 作者单位： | 遗传工程国家重点实验室 |
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| 基金项目： | 国家自然科学基金，国家"八六三"计划生物技术领域青年基金 |
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| 摘 要： | 血小板生成素（ｔｈｒｏｍ ｂｏｐｏｉｅｔｉｎ， ＴＰＯ）是调节血小板生成最主要的细胞因子，其生物学效应由其受体ｃ－Ｍｐｌ介导．利用酵母双杂合系统（ｔｗ ｏ－ｈｙｂｒｉｄ ｓｙｓｔｅｍ ）筛选与ｃ－Ｍｐｌ相互作用的蛋白质因子，以Ｇａｌ４ ＢＤ融合ｃ－Ｍｐｌ膜内部分ｃＤＮＡ 的ｐＡＳＭＭ 为靶蛋白质粒，筛选了人胎盘ｃＤＮＡ 文库，分离到人波形纤维蛋白（ｖｉｍ ｅｎｔｉｎ）的部分编码序列，首次检测到波形纤维蛋白与ＴＰＯ 受体之间的相互作用，这提示细胞骨架蛋白可能在ＴＰＯ 的信号转导过程中起着重要的作用
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| 关 键 词： | 血小板生成素受体c-Mpl 酵母双杂合系统 波形纤维蛋白 相互作用 |
Preliminary Study of Proteins that Interact with Human Thrombopoietin Receptor c-Mpl Screened by Yeast Two-Hybrid System |
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| ZHOU Wei guo,FENG Li bing,ZHAO Xin yan,DAI Wei lie,LI Chang ben,ZHAO Shou yuan.Preliminary Study of Proteins that Interact with Human Thrombopoietin Receptor c-Mpl Screened by Yeast Two-Hybrid System[J].Journal of Fudan University(Natural Science),1999(5). |
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| Authors: | ZHOU Wei guo FENG Li bing ZHAO Xin yan DAI Wei lie LI Chang ben ZHAO Shou yuan |
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| Abstract: | Thrombopoietin (TPO) is the major cytokine involved in platelet production and exerts its effects via the receptor c Mpl. The yeast two hybrid system has been used to screen the proteins interacting with c Mpl. The plasmid pASMM containing Gal 4 DNA binding domain fused with c Mpl intracellular domain was used as a target plasmid to screen a human placenta cDNA library, a part of coding sequence of vimentin was obtained. Therefore, it is the first time to detect an interaction between vimentin and TPO receptor. The results suggest that this cytoskeletal protein may play an important role in TPO signal transduction pathway. |
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| Keywords: | thrombopoietin receptor c-Mpl yeast two hybrid system vimentin interaction |
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