| 禽源致病性沙门氏菌四环素耐药性基因tetC的PCR扩增及序列分析 |
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| 引用本文： | 张正庆,罗薇,刘亚刚,于学辉,傅安静,黄兴.禽源致病性沙门氏菌四环素耐药性基因tetC的PCR扩增及序列分析[J].西南民族学院学报(自然科学版),2007,33(2):313-315. |
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| 作者姓名： | 张正庆 罗薇 刘亚刚 于学辉 傅安静 黄兴 |
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| 作者单位： | 西南民族大学生命科学与技术学院 成都610041 |
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| 摘 要： | 通过对临床分离并经生化实验和血清学试验鉴定的20株禽源沙门氏菌和1株标准株C79-13进行药敏试验,结果证明:有19株有高耐药性,另外2株有低耐药性,耐药率达100%;对其四环素耐药基因tetC进行了扩增,结果获得以质粒为模板的特异性产物,与药敏试验符合率为100%;利用PCR检测四环素耐药基因tetC具有很高的敏感性和特异性,从而为禽类致病性沙门氏菌的耐药性检测提供了高效敏感的手段.
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| 关 键 词： | 沙门氏菌 四环素耐药基因 TetC PCR |
| 文章编号： | 1003-2843（2007）02-0313-03 |
| 修稿时间： | 2006-10-12 |
PCR amplification and nucleotide sequence analysis of tetracycline resistance gene (tetC) of avian pathogenic salmonella |
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| ZHANG Zheng-qing,LUO Wei,LIU Ya-gang,YU Xue-hui,FU An-jing,HUANG Xing.PCR amplification and nucleotide sequence analysis of tetracycline resistance gene (tetC) of avian pathogenic salmonella[J].Journal of Southwest Nationalities College(Natural Science Edition),2007,33(2):313-315. |
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| Authors: | ZHANG Zheng-qing LUO Wei LIU Ya-gang YU Xue-hui FU An-jing HUANG Xing |
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| Affiliation: | School of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, E R. C. |
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| Abstract: | By using clinical isolation and biochemical and serologic test detection on 20 avian salmonella strains and one standard salmonella, the drug-susceptibility test shows that: 19 strains are with high resistance, and the other 2 are low. The drug-susceptibility tests also show that the rate is 100%. After amplifying the tetracycline-resistance gene, it produces specific material by plasmids complete and the concordance rate to drug-susceptibility test is 100%. The result indicates that the methods of PCR to detect tetracycline-resistant gene has the character of susceptibility and speciality. The paper provides effective and exact methods to detect the drug-resistance of avian salmonella. |
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| Keywords: | salmonella tetracycline-resistant gene tetC PCR |
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