| 反相高效液相色谱法测定栀子金花丸中栀子苷的含量 |
| |
| 引用本文： | 郭永成,王学蕾.反相高效液相色谱法测定栀子金花丸中栀子苷的含量[J].齐鲁药事,2006,25(2):95-96. |
| |
| 作者姓名： | 郭永成 王学蕾 |
| |
| 作者单位： | 临沂市中医医院,临沂,276002 |
| |
| 摘 要： | 目的采用反相高效液相色谱法测定栀子金花丸中栀子苷的含量。方法色谱柱为spherisorb C18(4.6 mm×250mm 5μm);流动相为甲醇-水(30:70),流速为1ml.min-1,检测波长为238nm。结果栀子苷在0.0472~0.236μg范围内线性关系良好,r=0.9999,平均回收率为101.9%,RSD为1.2%(n=5)。结论该方法简便、快速,重现性好。
|
| 关 键 词： | 反相高效液相色谱法 栀子金花丸 栀子苷 |
| 文章编号： | 1672-7738(2006)02-0095-02 |
Determination of Geniposide in Zhizi Jinhua pills by RP-HPLC |
| |
| GUO Yong-cheng,WANG Xue-lei.Determination of Geniposide in Zhizi Jinhua pills by RP-HPLC[J].qilu pharmaceutical affairs,2006,25(2):95-96. |
| |
| Authors: | GUO Yong-cheng WANG Xue-lei |
| |
| Abstract: | OBJECTIVE An RP-HPLC method for determination of Geniposide in Zhizi Jinhua pills was established.METHODS A spherisorb C_(18) column (4.6mm×250mm 5μm) was use with methanol-water(30:70) as mobile phase.The flow rate was 1ml·min~(-1).Geniposide content was detected at a wavelength of 238nm. RESULTS The linear range was 0.0472~0.236μg(r=0.9999).The average recovery was 101.9%,RSD was 1.2%(n=5). CONCLUSION The method was simple,rapid and reproducible. |
| |
| Keywords: | RP-HPLC Zhizi Jinhua pills geniposide |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
