慢性HBV感染者调节性T细胞水平及Foxp3与CD127表达关系的研究

目的 探讨慢性HBV感染者外周血中CD4^＋CD25^＋Foxp3^＋调节性T细胞（RegulatoryT,Treg）的水平,并研究其两种细胞标志--CD127分子的低表达与Foxp3转录因子表达之间的关系.方法 采集34例免疫耐受期、26例免疫清除期和31例非活动或低（非）复制期慢性HBV感染者的外周全血,用流式细胞术分析CD4^＋CD25^＋Foxp3^＋T细胞和CD4^＋CD25^＋CD127^（low）T细胞的频率.结果 在慢性HBV感染者外周血中,免疫耐受组CD4^＋CD25^＋Foxp3^＋T细胞和CD4^＋CD25^＋CD127^（low）T细胞的频率均比非活动或低（非）复制组高（Z=-2.693,P=0.007和t=3.251,P=0.002）;且病毒阳性组（免疫耐受组＋免疫清除组）比非活动或低（非）复制组高（t=2.266,P=0.026和t=3.208,P=0.002）;ALT异常组（免疫清除组）与ALT正常组（免疫耐受组＋非活动或低（非）复制组）之间的比较差异无统计学意义（P〉0.05）.91例病例外周血中CD4^＋CD25^＋Foxp3^＋T细胞和CD4^＋CD25^＋CD127^（low）T细胞之间的表达有一致性,但两者频率的差异有统计学意义（Z=-4.718,P〈0.001）.结论 调节性T细胞在慢性HBV感染者病毒高复制组中明显升高.CD4^＋CD25^＋T细胞中CD127分子的低表达与Foxp3转录因子的表达有一致性,但前者代表Treg的频率明显高于后者.

Studies about the level of CD4~+CD25~+ regulatory T cells and relation between expression of Foxp3 and CD127 in peripheral blood of chronic HBV infection

Objective To investigate the level of CD4~+CD25~+Foxp3~+ regulatory T cells and observe relation between expression of Foxp3 and CD127 in peripheral blood of chronic HBV infection. Methods CD4~+CD25~+Foxp3~+and CD4~+CD25~+CD127~(low) Treg in peripheral blood from 34 patients of immune tolerance stage, 26 patients of immune clearance stage and 31 patients of non-active status were quantitatively analyzed by flow cytometry. Results Immune tolerance group presented a higher fraction of CD4~+CD25~+Foxp3~+ and CD4~+CD25~+CD127~(low) Treg than non-active group in chronic HBV infection(Z= -2.693, P=0.007 and t=3.251, P=0.002 ), and HBV positive group also presented a higher fraction than non-active group(t=-2.266,P=0.026 and t=3.208,P=0.002), But ALT normal group is similar to ALT abnormal group(P>0.05). In this study, the relation between expression of CD127~(low) and Foxp3~+ from CD4~+ CD25~+ regulatory T cells was observed, and CD4~+ CD25~+ CD127~(low) Treg presented a higher fraction than CD4~+CD25~+ Foxp3~+ Treg. Conclusion Peripheral Treg in HBV active replication group is higher than HBV negative group of chronic HBV infection. Expression of CD127~(low) is consistent with Foxp3~+ in CD4~+CD25~+ regulatory T cells, but the former is significant higher than the later.