6株人源乳酸菌体外抗氧化活性的比较

本文对分离自江苏如皋长寿村人群肠道的6株乳酸菌体外抗氧化活性进行了研究。测定了6株菌对过氧化氢耐受能力、不同组分对羟自由基、DPPH自由基清除能力、还原能力、抗脂质过氧化能力及T-SOD和GSH-Px活性,结果表明:6株菌中,发酵乳杆菌L2、L4,屎肠球菌E2对1.0 mM H2O2耐受能力较强。6株菌不同组分均具有一定的抗氧化活性,发酵上清液的抗氧化活性总体要优于菌体和胞内提取物。其中,L2发酵上清液羟自由基清除能力和T-SOD活性最强;E2发酵上清液DPPH自由基清除能力和GSH-Px活性最高;发酵乳杆菌L1发酵上清液还原能力最高;干酪乳杆菌L3发酵上清液抗脂质过氧化能力最强。测定结果表明这6株菌总体抗氧化能力较好,具有潜在的应用价值。

In Vitro Antioxidant Activities of Six Lactic Acid Bacteria Isolated from Human Intestinal Tract

The antioxidant activities of 6 lactic acid bacteria isolated from the intestinal tract of the longevity village people of Jiangsu Rugao were studied by hydrogen peroxide tolerance assay, hydroxyl radical and DPPH radical scavenging assays, reducing activity assay, anti-lipid peroxidation assay, T-SOD and GSH-Px activity assays in this study. The results showed that L. fermentum L2, L. fermentum L4 and E. faecium E2 had higher resistant capability against 1.0 mM hydrogen peroxide than the others. Different constituents of 6 lactic acid bacteria had different antioxidant activities, among which the antioxidant activity of fermentation supernatant was superior to bacterial cell and intracellular extract. The fermentation supernatants of L. fermentum L2 showed the highest hydroxyl radical scavenging activity and T-SOD activity, while the fermentation supernatants of E. faecium E2 demonstrated the highest DPPH radical scavenging activity and GSH-Px activity. In addition, the fermentation supernatants of L. fermentum L1 had the highest reducing power; while the fermentation supernatants of L. casei L3 had the highest lipid peroxidation-inhibitory activity. Six lactic acid bacteria had good antioxidant capacity and could be used in future as potential probiotics.