扩散加权MRI活体评价外源野生型P53逆转SW480/5-FU人类结肠癌效果

目的探索扩散加权MRI(DWI)活体评价rAd/p53逆转耐5-FU结肠癌SW480/5-FU对5-FU耐药性效果的可能性。材料与方法 100只结肠癌SW480/5-FU荷瘤裸鼠随机分成4组,对照组、5-FU组、rAd/p53组、rAd/p53+5-FU组。各组在治疗后24 h、48 h、72 h、120 h、168 h分别随机取5只荷瘤鼠行DWI检查,随后处死荷瘤鼠,切下肿瘤检测肿瘤细胞凋亡(TUNNEL法)。结果 rAd/p53组与rAd/p53+5-FU组在观察时间点肿瘤ADC值均较对照组增加,与对照组比较差别有统计学意义(P0.05);rAd/p53组与rAd/p53+5-FU组在观察时间点肿瘤凋亡较对照组增加;rAd/p53+5-FU组在观察时间点肿瘤肿瘤凋亡和ADC值较rAd/p53组高。ADC值与肿瘤凋亡呈正相关。结论 ADC值能活体评价外源野生型p53逆转SW480/5-FU耐药性早期疗效。

In vivo detection of the reversal effects using rAd/p53 to human colon cancer in mice with diffusion-weighted magnetic resonance imaging

Objective:To investigate the potential of diffusion-weighted magnetic resonance imaging (DWI) to detect the reversal effects of rAd/p53 for 5-FU resistance ofhumancoloncancerinvivo.Materials and Methods:Onehundrednudemicewith human colon cancer SW480/5-Fu (5-Fu resistance) were randomly assigned into four groups:control group, 5-FU group, rAd/p53 group, rAd/p53+5-FU. At 24 h, 48 h, 72 h, 120 h and 168 h after treatment, 5 mice were selected randomly from each group and given DWI examinations. Then these mouse models were sacrificed with an overdose of anesthetic. Their tumors were removed and detected apoptosis (TUNNEL). Results:The ADC values at the edge area of the tumor in rAd/p53 and rAd/p53+5-FU groups were significantly higher than that of the control groups at the observed time points (P<0.05). The area ratios of tumor cell apoptosis in the rAd/p53+5-FU and rAd/p53 group were larger than that of control groups at the observed time point. The area ratios of tumor cell apoptosis and the ADC values in the rAd/p53+5-FU group were larger than that of rAd/p53 group at the observed time point. The ADC values of tumors were positively correlated with the area ratios of tumor cell apoptosis. Conclusions:The ADC value can evaluate the reversal effects of drug resistance with rAd/p53 in early stage of therapy in vivo.