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| The Effect of HBx Gene on the Apoptosis of Hepatic Cells | |
| 作者姓名： | 叶露 齐俊英 李高鹏 陶德定 宋世会 |
| 作者单位： | Department of Infection Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Infection Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Hepatic Surgery Center Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Molecular Medicine Center Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Infection Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China |
| 摘 要： | To study the effect of HBx gene on the apoptosis of the cell lines (L02, HepG2) and the interaction between HBx and X-linked inhibitor of apoptosis protein (XIAP), the apoptosis of pcDNA3.1-HBx transiently transfected cell lines (L02, HepG2) was detected by flow cytometry and the mRNA expression of XIAP was assayed by real-time RT-PCR. Our study showed (1) the mor- phology of L02/pcDNA3.1-HBx was changed and the appearance of the cells mimicked that of HepG2 cells; (2) HBx gene could be detected in L02/pcDNA3.1-HBx and HepG2/ pcDNA3.1-HBx; (3) the apoptosis rate of L02/pcDNA 3.1-HBx was higher than that of L02 cells (P<0.01) and the apoptosis rate of HepG2/pcDNA3.1-HBx was lower than that of HepG2 cells (P<0.05); (4) the XIAP expression in L02 was about 3 times that in L02/pcDNA3.1-HBx cells (P<0.01), and the expression of XIAP in HepG2/pcDNA3.1-HBx was about 4 times that in HepG2 (P<0.01). It is concluded that HBx gene may promote the apoptosis of normal hepatocytes and inhibit the apoptosis of cells of he- patic carcinoma by regulating the expression of XIAP. |
| 关 键 词： | HBx基因 肝细胞凋亡 XIAP IAPs 肝硬化 |
| 收稿时间： | 2006-08-20 |
The effect of HBx gene on the apoptosis of hepatic cells |
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| Ye Lu,Qi Junying,Li Gaopeng,Tao Deding,Song Shihui.The Effect of HBx Gene on the Apoptosis of Hepatic Cells[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2007,27(2):167-169. | |
| Authors: | Ye Lu Qi Junying Li Gaopeng Tao Deding Song Shihui |
| Affiliation: | 1. Department of Infection, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology, Wuhan 430030, China 2. Department of Hepatic Surgefy Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China 3. Department of Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
| Abstract: | To study the effect of HBx gene on the apoptosis of the cell lines (L02, HepG2) and the interaction between HBx and X-linked inhibitor of apoptosis protein (XIAP), the apoptosis of pcDNA3.1-HBx transiently transfected cell lines (L02, HepG2) was detected by flow cytometry and the mRNA expression of XIAP was assayed by real-time RT-PCR. Our study showed (1) the mor- phology of L02/pcDNA3.1-HBx was changed and the appearance of the cells mimicked that of HepG2 cells; (2) HBx gene could be detected in L02/pcDNA3.1-HBx and HepG2/ pcDNA3.1-HBx; (3) the apoptosis rate of L02/pcDNA 3.1-HBx was higher than that of L02 cells (P<0.01) and the apoptosis rate of HepG2/pcDNA3.1-HBx was lower than that of HepG2 cells (P<0.05); (4) the XIAP expression in L02 was about 3 times that in L02/pcDNA3.1-HBx cells (P<0.01), and the expression of XIAP in HepG2/pcDNA3.1-HBx was about 4 times that in HepG2 (P<0.01). It is concluded that HBx gene may promote the apoptosis of normal hepatocytes and inhibit the apoptosis of cells of he- patic carcinoma by regulating the expression of XIAP. |
| Keywords: | HBx apoptosis IAPs XIAP HCC |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ | |
