| 人型结核杆菌H_(37)Rv株菌体特异性抗原TB4单克隆抗体的纯化 |
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| 引用本文： | 陆学东,孙连魁,陈英,张银辉.人型结核杆菌H_(37)Rv株菌体特异性抗原TB4单克隆抗体的纯化[J].西北大学学报,1999(5). |
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| 作者姓名： | 陆学东 孙连魁 陈英 张银辉 |
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| 作者单位： | 陕西省人民医院老年病研究中心!陕西西安710068(陆学东，张银辉)，西北大学生物学系!陕西西安710069(孙连魁，陈英) |
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| 摘 要： | 采用硫酸铵法、辛酸二步法、ＤＥＡＥ－Ｓｅｐｈａｄｅｘ Ａ５０ 离子交换柱层析法，从杂交瘤小鼠腹水中纯化人型结核杆菌Ｈ３７Ｒｖ 株特异性抗原ＴＢ４ 的单克隆抗体，从回收率、纯度和活性３ 个方面进行比较。结果显示，硫酸铵法回收率最高，层析法纯度最高，３ 种方法对单抗的活性均无大的影响。认为：辛酸二步法可有效地去除白蛋白，适用于大量腹水中单抗的提取、纯化，具有简单、省时等优点；柱层析法适用于小量腹水单抗纯化及分析用途
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| 关 键 词： | 结核杆菌 抗原 单克隆抗体 纯化 |
Purification of monoclonal antibody against TB4 antigen of mycobacterium tuberculosis H_(37) Rv Strain |
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| LU Xue dong ,SUN Lian kui ,CHEN Ying ,ZHANG Yin hui.Purification of monoclonal antibody against TB4 antigen of mycobacterium tuberculosis H_(37) Rv Strain[J].Journal of Northwest University(Natural Science Edition),1999(5). |
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| Authors: | LU Xue dong SUN Lian kui CHEN Ying ZHANG Yin hui |
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| Affiliation: | LU Xue dong 1,SUN Lian kui 2,CHEN Ying 2,ZHANG Yin hui 1 |
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| Abstract: | Ammonium sulfate,caprylic acid precipitation and ion exchange chromatographical methods have been used to purify monoclonal antibody(McAb) against TB4 antigen of Mycobacterium tuberculosis H 37 Rv strain from ascitic fluid in Balb/c mice.The results showed that ammonium sulfate precipitation method could have the highest harvest rate and ion exchange chromatography method could have the higest purity.These methods of purifying McAb could not impair the antibody activety.Caprylic acid method can remove albumin effectively and the purification of anti TB4 McAb in ascitic fluid is simple and easy to perform,less time comsuming and less cost.Ion exchange chromatographical method is applicable to analysis. |
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| Keywords: | mycobacterium tuberculosis antigen monoclonal antibody purification |
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