| 绿原酸的分离纯化工艺研究 |
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| 引用本文： | 刘畅,苟建敏,何珺.绿原酸的分离纯化工艺研究[J].贵州大学学报(自然科学版),2011,28(6):29-32. |
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| 作者姓名： | 刘畅 苟建敏 何珺 |
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| 作者单位： | 1. 贵州大学生命科学学院,贵州贵阳,550025
2. 贵州省生化工程中心,贵州贵阳,550025 |
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| 摘 要： | 研究从杜仲提取物中分离纯化绿原酸的工艺,确定其分离纯化参数.采用料液比1∶20水超声提取,上聚酰胺柱分离,4BV15％乙醇洗脱,洗脱浓缩液调pH为1-2静置结晶,可得85％以上的绿原酸粗晶,再甲醇重结晶,可得97％绿原酸白色针状晶体.采用此工艺可以将杜仲提取物中绿原酸由20％分离纯化至97％,工艺适合工业化生产.
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| 关 键 词： | 杜仲提取物 绿原酸 分离 提纯 |
Research on Separation and Purification of Chlorogenic Acid from Extraction of Eucommia Ulmoides |
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| LIU Chang
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GOU Jian-min
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HE Jun.Research on Separation and Purification of Chlorogenic Acid from Extraction of Eucommia Ulmoides[J].Journal of Guizhou University(Natural Science),2011,28(6):29-32. |
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| Authors: | LIU Chang GOU Jian-min HE Jun |
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| Affiliation: | Jun ( 1. College of Life Science, Guizhou University, Guiyang 550025, China ;2. Research Center of Biochemistry Engineering of Guizhou Province, Guizhou University, Guiyang 550025, China) |
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| Abstract: | The paper aims to study the separation and purification process of Chlorogenic acid from the Extractionof Eucommia ulmoide. Using 15% alcohol-water could wash chlorogenic acid effectively. The PH of the concentrated solution was adjusted to 1 or 2 and was put tals can be obtained by crystallizing in extraction was obtained through recrystallizing rough crystals fy the chlorogenic acid of Extraction of Eucommia it in shade for crystallization. The 85% chlorogenic acid cryslayer, the 97% white needle-like crystals of chlorogenic acid in methanol-water system. This technique can be used to purifrom 20% to 97%, which suitable for industrial production. |
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| Keywords: | Extraction of Eucommia ulmoides Chlorogenic acid separation purification |
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