灯盏乙素对缺氧缺血性新生大鼠脑缺氧诱导因子-1α和水通道蛋白9表达水平的影响

目的：研究灯盏乙素对缺氧缺血性脑损伤（HIBD）新生大鼠脑缺氧诱导因子-1α（HIF-1α）和水通道蛋白9（APQ9）表达水平的影响。方法：建立HIBD新生大鼠模型，将新生7日龄SD大鼠随机分为假手术组（sham组）、缺氧缺血组（HIBD组）、灯盏乙素高、中和低剂量组、地塞米松阳性对照组。于缺氧缺血7 d后，观察大鼠体质量增长率，脑组织HE染色观察脑组织病理改变，测定脑含水量，免疫荧光法检测脑HIF-1α蛋白表达，逆转录-多聚酶链反应（RT-PCR）法检测脑HIF-1α、APQ9mRNA表达，ELISA法检测脑caspase-3活性。结果：灯盏乙素能显著升高HIBD大鼠体质量增长率，降低脑组织含水量，抑制脑组织HIF-1α和AQP9mRNA表达，降低脑caspase-3活性。结论：灯盏乙素可减轻HIBD大鼠脑组织水肿，降低HIF-1α和AQP9表达，抑制神经细胞凋亡，发挥脑保护作用。

The effect of scutellarin on brain tissue hypoxia-inducible factor 1α and aquaporin 9 expression in neonatal rats with hypoxia-ischemia brain damage

OBJECTIVE To explore the effect of scutellarin on the expression of hypoxia-induced factor-1α (HIF-1α) and aquaporin 9 (AQP9) in neonatal rats brain after hypoxia-ischemia brain damage. METHODS Models of 7-day-old neonatal rats with HIBD were established. The rats were randomly divided into six groups:the sham group, hypoxic-ischemic brain damage (HIBD) group, scutellarin treated groups of three different doses, and dexamethasone group. After HIBD for 7 days, the brain water content and weight growth rate were detected by physical methods. The brain tissue morphological changes were observed by HE staining. The HIF-1α and AQP9 mRNA were measured by RT-PCR. The expression of HIF-1α was observed by immunofluorescence staining, and brain caspase-3 activity was measured by ELISA. RESULTS The brain water content in scutellarin treated groups decreased. Weight growth rate in scutellarin treated groups increased. HIF-1α and AQP9 mRNA expressions were inhibited by scutellarin. Caspase-3 activity in scutellarin treated groups decreased. CONCLUSION Scutellarin protects the brain tissues with HIBD probably through the relief of cerebral edema, down regulation of the expression of HIF-1α and AQP9, and inhibition of cell apoptosis.