Foxp3在卵巢癌组织中的表达及临床意义

目的 探讨叉状头-翅膀状螺旋转录因子p3（Forkhead box protein 3，Foxp3）在卵巢癌组织中的表达及其与预后的关系。方法 采用免疫组化染色法检测卵巢癌组织芯片中45例癌组织及7例正常卵巢组织的Foxp3表达。应用秩和检验比较不同病理类型卵巢癌Foxp3表达的差异；卡方检验分析Foxp3表达与临床病理参数的关系；Kaplan-Meier法分析Foxp3表达与卵巢癌预后关系；Cox比例风险回归模型评价影响患者预后的因素。结果 Foxp3阳性着色主要定位于细胞核。Foxp3表达与FIGO分期有关（P＜0.05），与年龄、病理类型、分化程度、肿瘤长径、淋巴结转移等均无关（P＞0.05）。Kaplan-Meier法生存分析显示Foxp3低表达者（H-score≤90）中位生存时间（OS）为6183个月，显著高于高表达者（H-score＞90）的25.03个月（P＜0.05）。Cox比例风险模型显示，术后未化疗（HR=6.603，95％CI： 1.147～32.057）、FIGO分期较高（HR=3.861，95％CI： 1.427～10.417） 为预测卵巢癌不良预后的独立因素。结论 卵巢癌组织中Foxp3表达与FIGO分期相关，并可以作为卵巢癌预后判断的参考指标。

Expression of Foxp3 in ovarian cancer and its clinical significance

Objective To investigate the expression of Forkhed box protein 3(Foxp3) in ovarian cancer and its relationship with prognosis. Methods Expression of Foxp3 was assessed by immunohistochemistry in tissue microarrays containing tumor tissues from 45 ovarian patients and 7 normal ovarian tissues. The rank sum test was utilized to compare the differences between different path-ological types. Chi-square test was utilized to analyze recationship between Foxp3 expression and clinicopathological features. Kaplan-Meier method was utilized to analyze the correlation between Foxp3 expression and prognosis. The multi-factor Cox proportional hazards model was used to estimate the intensity of association between different clinicopathological features and mortal outcomes. Results The Foxp3 expression was located in nucleus of tumor cells. The expression of Foxp3 was related to FIGO stage(P<0. 05), but age, patho-logical type, differentiation, tumor size and lymph node metastasis(P>0. 05). Kaplan-Meier method revealed that patients with lower Foxp3 expression had prolonged survival time than that with higher Foxp3 expression(61. 83 months vs. 25. 03 months, P= 0. 03). The Cox regression model multifactor analyze showed that without postoperative chemotherapy(HR = 6. 603, 95%CI: 1. 147-32. 057, P =0. 03), higher FIGO stage(HR = 3. 861, 95%CI: 1. 427-10. 417, P<0. 01) were independent prognostic factors of ovarian cancer. Conclusion Expression of Foxp3 was related to FIGO stage, and can predict survival of ovarian cancer.