| 高效液相色谱法测定金玉软坚片中栀子苷含量 |
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| 引用本文： | 王茉,亓展.高效液相色谱法测定金玉软坚片中栀子苷含量[J].中国药业,2012,21(7):19-20. |
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| 作者姓名： | 王茉 亓展 |
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| 作者单位： | 1. 吉林省吉林市食品药品检验所,吉林吉林,132001
2. 黑龙江省哈尔滨市第四医院,黑龙江哈尔滨,150026 |
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| 摘 要： | 目的 建立金玉软坚片的含量测定方法.方法 采用高效液相色谱法.色谱柱为Phenomenex C18柱(250mm×4.6mm,5μm),流动相为乙腈-0.05%磷酸溶液(12:88),流速1.0 mL/min,检测波长238 am,柱温40℃.结果 栀子苷质量浓度在14.96～74.80 μg/mL范围内与峰面积线性关系良好(r＝0.999 9);平均加样回收率为99.53%,RSD=1.50%(n=6).结论 该方法简便易行,结果准确可靠,可适用于金玉软坚片的质量控制.
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| 关 键 词： | 金玉软坚片 栀子苷 高效液相色谱法 含量测定 |
Determination of Geniposide in Jinyuruanjian Tablets by HPLC |
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| Wang Mo
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Qi Zhan.Determination of Geniposide in Jinyuruanjian Tablets by HPLC[J].China Pharmaceuticals,2012,21(7):19-20. |
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| Authors: | Wang Mo Qi Zhan |
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| Affiliation: | 1. Jilin Institute for Food and Drug Control, Jilin, Jilin, China 132001; 2. No. 4 Hospital of Harbin, Harbin, Heilong]iang, China 150026) |
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| Abstract: | Objective To set up a method for quality control of Jinyuruanjian Tablets. Methods HPLC method was developed to quan- titative determination. Phenomenex Cls column （250 mm × 4. 6 mm, 5 μm）. The mobile phase was acetonitrile -0. 05% phosphoric acid solution （12：88）. The column temperature was 40 ℃,the wavelength for detection was 238 nm,flow rate was 1.0 mL/min. Results The good linear relationship was found for geniposide in the concentration range of 14.96-74.80 μg/mL（r =0. 999 9）. The geniposide average of recovery rate was 99.53%, and RSD was 1.50% （n =6）. Conclusion The method is simple,accurate and suitable for its assaying. |
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| Keywords: | Jinyuruanjian Tablets geniposide HPLC content determination |
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