降脂壮骨方剂含药血清对大鼠骨髓基质细胞骨形态发生蛋白-2表达的影响

目的研究高胆固醇血清以及降脂壮骨方剂含药血清干预下大鼠骨髓基质细胞骨形态发生蛋白-2（BMP-2）表达的变化。方法取成年大鼠股骨，用密度梯度离心方法分离大鼠骨髓基质细胞，原代培养细胞至第三代，并用流式细胞仪进行细胞鉴定。选取第三代细胞，随机分为三组。A组正常血清对照组：2％空白血清（体积比）；B组高胆固醇血清损伤组：终浓度为4mmol／L高胆固醇血清；C组中药血清预处理＋高胆固醇血清损伤组：2％中药血清（体积比）预处理2h后＋终浓度为4mmol／L高胆固醇血清。回收细胞提取总RNA，检测BMP-2表达变化，并进行统计学分析。结果与空白血清对照组相比，高胆固醇血清损伤组骨髓基质细胞增殖受抑制；BMP-2的mRNA表达水平降低（P〈0．01）。与高胆固醇血清损伤组相比，中药血清预处理＋高胆固醇血清损伤组骨髓基质细胞生长状态明显改善，BMP-2的mRNA表达水平增高（P〈0．01）。结论降脂壮骨方剂含药血清可以增强高胆固醇血清环境下骨髓基质细胞BMP-2、雌激素受体（ER），明显改善骨髓基质细胞生长状态，有利于骨质疏松症的治疗。

Effect of the serum containing Jiang Zhi Zhuang Gu solution on BMP-2 expression of rat bone marrow stromal cells

Objective To study osteoporosis associated with hyperlipidemia;To research high cholesterol se-rum and Jiangzhizhuanggu-containing serum intervention rat bone marrow stromal cells BMP-2 expression changes. Methods Adult rat femur by density gradient centrifugation separation of bone marrow stromal cells, cultured cells to the third generation, and was identified by flow cytometry. The third generation cells, were randomly divided into three groups. Normal serum control group: two percent blank senun (volume ratio); High cholesterol serum injury group: fi-nal concentration of 4 mmol/L, high cholesterol serum; medicine + pretreatment serum high cholesterol serum injury, group :2% senun Chinese medicine(volume ratio) after pretreatment 2 hours + final concentration of 4 mmol/L, high cholesterol serum. Total RNA was isolated from cells recovered. BMP-2 mRNA expression was detected. And analyzed statistically. Results (1) Blank serum compared with the control group,high cholesterol serum cultured bone mar-row stromal cells proliferation was inhibited. BMP-2 mRNA expression significantly decreased(P < 0.01) . (2) Jiang-zhizhuanggu solution containing serum protection two hours later to join a high cholesterol serum group, the cell growth conditions improved significantly, BMP-2 mRNA expression significantly increased than pure high cholesterol serum injured group(P <0.01) . Conclusion Solution's Jiangzhizhuanggu containing serum can enhance high cholesterol serum environment bone manow stromal cells BMP-2 mRNA expression, significantly improved bone marrow stromal cell growth state is conducive to the treatment of osteoporosis.