基于16S rDNA测序研究白酒对大鼠肠道菌群的影响

为探究浓香型白酒对SD大鼠肠道微生态的影响，通过连续8周灌胃适当剂量乙醇，建立SD大鼠慢性酒精性肝损伤模型，同时，灌胃相同剂量的白酒，考察白酒和乙醇对SD大鼠血脂、肝脏指标的影响及造成的肠道菌群变化。结果显示，连续8周灌胃乙醇造成SD大鼠一定程度的酒精性肝损伤，而白酒组的肝损伤程度显著低于乙醇组。多样性分析表明乙醇组SD大鼠肠道菌群丰富度和多样性降低，而白酒的摄入可以回调这种现象。菌群结构分析表明乙醇干预和白酒干预之间存在显著差异，其中白酒干预组物种组成与对照组更加接近。差异性分析筛选出白酒组和乙醇组的3个潜在差异微生物标志物，分别为g_Eubacterium_rumminantium_group、g_U29-B03和g_unclassified_f_Oscillospiraceae。该研究结果表明白酒可能通过调节肠道微生态来减轻乙醇诱导的轻度肝损伤。

Effect of Baijiu on Gut Microbiota of Rats Using 16S rDNA Sequencing

This study was conducted to investigate the effects of Nongxiangxing Baijiu on the gut microbiota in SD rats. A SD rat model of chronic alcoholic-induced liver injury was established by intragastrically administering with appropriate doses of ethanol for 8 weeks. At the same time, an equivalent dose of Baijiu was administered intragastrically to investigate the effects of Baijiu and ethanol on blood lipids and liver indicators of SD rats, and the changes in gut microbiota. The results showed that the intragastric administration of ethanol for 8 consecutive weeks caused a certain degree of alcoholic liver injury to SD rats, while the severity of liver damage was significantly lower in the Baijiu group compared to the ethanol group. The diversity analysis revealed that the ethanol intervention resulted in decreased abundance and diversity of the gut microbiota of SD rats, while the intake of Baijiu could reverse this phenomenon. Moreover, the analysis of microbial community structure demonstrated significant differences between the ethanol and Baijiu interventions, with the species composition in the Baijiu intervention group being closer to that of the control group. Three potential differential microbial biomarkers in the Baijiu and ethanol groups were identified by difference analysis, which were g_Eubacterium_rumminantium_group, g_U29-B03 and g_unclassified_f_Oscillospiraceae. These results indicate that Baijiu may alleviate the mild ethanol-induced liver injury by regulating the gut microbiota.