| RAPD标记在烟草研究中的应用 |
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| 引用本文： | 时焦,徐建华,赖禄祥,韩锦峰.RAPD标记在烟草研究中的应用[J].中国烟草学报,2002,8(1):34-38. |
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| 作者姓名： | 时焦 徐建华 赖禄祥 韩锦峰 |
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| 作者单位： | 1.河南农业大学, 河南郑州 450002; |
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| 摘 要： | 随机扩增多态DNA(Randomly Amplified Polymorphic DNAs,RAPD)分子标记技术的诞生,为分子生物学技术在农业研究中的应用注入了新的活力,并为传统农业研究向分子水平发展提供了捷径。本文重点综述了RAPD分子标记技术在烟草群体分离分析和鉴定、标记辅助选择、遗传变异的识别、烤烟品种分子指纹分析、黑胚病菌全基因组DNA多态性标记、根结线虫种类鉴定、赤星病菌的分类地位等方面的研究进展,以及这些领域的研究发展和应用前景。
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| 关 键 词： | 烟草 RAPD 分子标记 |
| 文章编号： | 1004-5708(2002)01-0034-05 |
| 收稿时间： | 2000-07-24 |
| 修稿时间： | 2000年7月24日 |
On the application of RAPD techniques in tobacco research |
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| Shi Jiao Xu Jianhua Lai Luxiang Han Jinfeng Henan Agricultural University,Henan Zhengzhou Qingzhou Tobacco Research Institute of CNTC,Shandong Qingzhou Sanming Division of Fujian Tobacco Company,Fujian Sanming.On the application of RAPD techniques in tobacco research[J].Acta Tabacaria Sinica,2002,8(1):34-38. |
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| Authors: | Shi Jiao Xu Jianhua Lai Luxiang Han Jinfeng Henan Agricultural University Henan Zhengzhou Qingzhou Tobacco Research Institute of CNTC Shandong Qingzhou Sanming Division of Fujian Tobacco Company Fujian Sanming |
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| Affiliation: | 1.Henan Agricultural University, Henan Zhengzhou 450002;2.Qingzhou Tobacco Research Institute of CNTC, Shandong Qingzhou 262500;3.Sanming Division of Fujian Tobacco Company, Fujian Sanming 365001 |
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| Abstract: | The technique of Randomly Amplified Polymorphic DNAs (RAPD) has been widely used in tobacco (Nicotinana tobaccum) research. Such as bulk segregant analyses and gene identification, marker assisted selection, molecular genetic variation, molecular fingerprints and genetic relationships among flue-cured tobacco varieties, genetic diversity of Phytophthora parasitica var. Nicotinae, identification of Meloidogyne spp. , the classification of Alternaria alternata. The future endeavors of RAPD technique in tobacco research was discussed. |
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| Keywords: | Tobacco RAPD Molecular marker |
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