CIK细胞对人肺腺癌AS49／DDP耐药细胞株的抗增殖及诱导凋亡形态学研究

目的研究多种细胞因子诱导的杀伤细胞（CIK）对A549／DDP肺癌耐药细胞株的抗增殖和诱导凋亡作用。方法应用细胞计数法设计细胞浓度，通过相差显微镜下观察CIK细胞对A549／DDP肺癌耐药细胞株形态学影响。结果随着CIK细胞与肺癌细胞效靶比增加及作用时间延长，抑制率明显增强，CIK细胞作用于肺癌细胞24h后，形态学观察肺癌细胞发生凋亡或坏死。结论体外制备的CIK细胞对肺癌耐药细胞株A549／DDP具有抑制增殖和促进凋亡作用。

Anti-proliferation and inducing apoptesis effects of ClK cells on lung cancer cell lines Wu

Objective To study the anti-proliferation and inducing apoptosis effects of cytokine-in- duced killer cells （CIK cells） on A549/DDP multidrug resistance lung cancer cell lines. Methods De- sign of concentration cell by cell counting, the anti-proliferation effects of CIK cells on A549/DDP lung cancer line were detected by cell counting method, and the morphological changes of the apoptosis cell were observed by the phase contrast microscope. Results The inhibitory rate enhanced obviously with the addition of effect / target rate and the extension of reaction time. Lung cancer cell apoptosis or necrosis was Morphologically observed after the lung cancer cells had been induced by the CIK cells for 24 hours. Conclusion The CIK cells are highly effective cells which have potent anti-proliferation and inducing anovtosis effects on the lung cancer cells.