次声对小鼠脑组织氧化状态的影响及姜黄素的脑保护效应观察

目的观察小鼠经8Hz／16Hz，130dB次声作用后脑超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）及丙二醛（MDA）的变化，并同时观察姜黄素的脑保护作用。方法选取72只BALB／C小鼠，将其随机分为空白对照组、单纯次声组及姜黄素＋次声组（包括高、中、低剂量亚组）。姜黄素＋次声组小鼠每日给予不同剂量的姜黄素干预，7d后停止给药；随后将各组小鼠置于8Hz或16Hz，130dB的次声环境中，每天持续2h，7d后处死小鼠，测定其脑组织中SOD、GSH-Px及MDA的变化情况，并进行组间结果对比。结果8Hz次声实验部分：与空白对照组比较，单纯次声组小鼠脑组织SOD及GSH-Px活性下降，MDA含量上升（P〈0．05），次声＋姜黄素中、高剂量组与单纯次声组比较，前者脑组织中GSH-Px及SOD活性上升，MDA含量下降（P〈0．05）。16Hz次声实验部分：与空白对照组比较，单纯次声组小鼠脑组织GSH-Px活性及MDA含量上升（P〈0．05），次声＋姜黄素中、高剂量组与单纯次声组比较，前者脑组织中GSH-Px活性及MDA含量均显著下降（P〈0．05）。结论8Hz／16Hz，130dB次声可导致小鼠脑组织GSH-Px、SOD活性变化及脑组织过氧化损伤，不同频率次声对脑组织抗氧化酶的影响作用不同，姜黄素可通过抗氧化作用缓解因次声诱发的脑损伤。

The oxidation balance of the mouse brain after infrasound exposure and the preventative effects of Curcumin

Objective To explore changes in the levels of superoxide dismutase (SOD) ,glutathione peroxi-dase(GSH-Px) and malondialdehyde (MDA) in the brains of mice after exposure to 8 Hz and 16 Hz infrasound at 130 dB, and effecls of Cureumin. Methods BALB/C mice were given various doses of Curcumin daily for 7 d. They were then exposed to 8 Hz or 16 Hz infrasound at 130 dB 2 h per day for 7 d. The mice were then sacrificed and the expression of SOD, GSH-Px and MDA in their brains was measured. Results The 8Hz group showed decreased expression of GSH-Px and SOD,while MDA expression was increased in comparison to a control group. In rats which had been treated with Curcumin, expression of GSH-Px and SOD was increased, and MDA was decreased compared with the untreated mice and the controls. With 16 Hz infrasound, the expression of GSH-Px and MDA was increased in the infrasound group compared with the controls. In the mice treated with Curcumin, the expression of GSH-Px and MDA was lower than in the untreated mice. Conclusion Eight Hz or 16 Hz infrasound at 130 dB may induce changes in GSH-Px and SOD expression and peroxidation in the brains of mice. The effects of antioxidase depend on the frequency. Curcumin can alleviate the damage through antiperoxidation.