电针"关元"与"三阴交"对围绝经期模型大鼠性激素及下丘脑βEP调整作用的比较

目的:探讨电针"关元""三阴交"对围绝经期模型大鼠神经内分泌的调整作用及二穴间相互影响.方法:复制大鼠去卵巢模型,测定电针各单穴及配伍后对模型大鼠血清雌二醇(E2)、促卵泡激素(FSH)、促黄体生成素(LH)、下丘脑β-EP、子宫脏器指数、子宫内膜厚度的影响.结果:电针能降低围绝经期模型大鼠血清LH、FSH含量,升高血清E2和下丘脑β-EP的含量,增加子宫脏器指数和子宫内膜厚度.在对下丘脑β-EP的影响上,二穴配伍作用强于任一单穴.结论:在对下丘脑β-EP的影响方面,"关元"与"三阴交"存在协同作用.     

Potential advantages of a combination of Chinese Medicine and bone marrow mesenchymal stem cell transplantation for removing blood stasis and stimulating neogenesis during ischemic stroke treatment

Combined treatment of ischemic stroke with Chinese medicine and exogenous bone marrow mesenchymal stem cell (BMSC) transplantation may improve the removal of blood stasis and stimulation of neogenesis.Chinese medicines that remove blood stasis not only promote blood circulation but also calm the endopathic wind,remove heat,resolve phlegm,remove toxic substances and strengthen body resistance.The medicinal targeting effect of Chinese medicine can promote the homing of BMSCs,and the synergistic therapeutic effects of drugs can contribute to BMSC differentiation.As such,exogenous BMSC transplantation has potential advantages for neogenesis.Chinese medicines and exogenous BMSCs provide complementary functions for the removal of blood stasis and stimulation of neogenesis.Therefore,a combination of Chinese medicine and transplantation of exogenous BMSCs may be particularly suited to ischemic stroke treatment.     

Potential advantages of a combination of Chinese Medicine and bone marrow mesenchymal stem cell transplantation for removing blood stasis and stimulating neogenesis during ischemic stroke treatment

Combined treatment of ischemic stroke with Chinese medicine and exogenous bone marrow mesenchymal stem cell(BMSC) transplantation may improve the removal of blood stasis and stimulation of neogenesis.Chinese medicines that remove blood stasis not only promote blood circulation but also calm the endopathic wind,remove heat,resolve phlegm,remove toxic substances and strengthen body resistance.The medicinal targeting effect of Chinese medicine can promote the homing of BMSCs,and the synergistic therapeutic effects of drugs can contribute to BMSC differentiation.As such,exogenous BMSC transplantation has potential advantages for neogenesis.Chinese medicines and exogenous BMSCs provide complementary functions for the removal of blood stasis and stimulation of neogenesis.Therefore,a combination of Chinese medicine and transplantation of exogenous BMSCs may be particularly suited to ischemic stroke treatment.     

促甲状腺素吖啶酯化学发光免疫分析方法的建立

目的 建立一种定量检测人血清促甲状腺素(TSH)的方法.方法 根据双抗体夹心法实验原理,分别用吖啶酯和生物素标记两株不同的单克隆抗体,链霉亲和素包被微粒,通过链霉亲和素-生物素系统分离固相和液相完成检测.结果 本方法的最低检测限为0.01 μIU/mL,线性范围0.3～60μIU/mL,批内变异为1.9％ ～2.3％,总变异为6.4％ ～8.6％,与ROCHE公司的促甲状腺素检测试剂盒(电化学发光法)比对,相关系数r=0.9935.结果 本方法的各项指标均能满足临床检测的要求,可以临床推广应用.     

医科院校直属附院党务工作者激励保障机制构建探析

针对目前医科院校直属附院普遍存在忽视党务工作及对党务干部队伍的建设和激励机制重视不够的问题,本文拟结合广西医科院校直属附院党务工作的实际情况,探索医院对党务工作者的激励保障机制。     

KGF及其联合G-CSF对~(137)Cs γ射线5.0Gy照射小鼠重要脏器的放射防护作用

目的研究角质细胞生长因子(KGF)联合粒细胞集落刺激因子(G-CSF)对受照小鼠造血及重要脏器的放射防护作用。方法将6至8周龄C57BL/6小鼠共70只随机分成5组:空白对照组、单纯照射组、KGF给药组、G-CSF给药组、KGF联合G-CSF给药组,经137Cs-γ射线一次性全身照射后分别给药,通过体重、脏器指数、各脏器SOD活性、MDA含量和外周血(WBC、RBC、Hb、PLT)等指标分析KGF及G-CSF对其血液和重要脏器的影响。结果受照后,KGF组与G-CSF组小鼠体重回升较明显;与G-CSF组相比,联合组肺指数差异有统计学意义(P0.05);外周血RBC、Hb、PLT的含量指标中,联合组高于其余各给药组;G-CSF组肝、心的SOD活性高于KGF组和联合组;KGF组的肺SOD活性高于G-CSF组和联合组。结论本研究表明联合组对小鼠造血系统的修复优于其他给药组;GCSF对于肝脏和心脏的抗氧化能力更有效;KGF对于肺部的抗氧化能力更有效。     

大脑皮层电刺激对偏瘫患者肢体功能活动的影响

目的探讨大脑皮层电刺激对偏瘫患者肢体功能的影响。方法脑血管后遗症患者随机分为3组,分别进行大脑皮层电刺激、周围神经和肌肉电刺激治疗和脑保护治疗,应用偏瘫后神经功能障碍评分比较三者的疗效。结果皮层刺激组上肢运动障碍、下肢运动障碍、肌张力增强和肢体疼痛的改善得分分别为:0.66±0.37、0.87±0.36、0.66±0.51和1.20±0.44,与内科治疗组比较P<0.01,与周围刺激组比较P<0.05。结论大脑皮层电磁激治疗在运动障碍、肌张力增强、肢体疼痛方面均具有非常显著的疗效。     

Stimulating catheter as a tool to evaluate peripheral nerve function during hip rotationplasty

OBJECTIVE: Stimulating catheters have been introduced into clinical practice to confirm perineural localization of the catheters. The muscular twitch induced over the catheter may be used to evaluate nerve function intraoperatively. Therefore, the function of the sciatic nerve was evaluated during major cancer surgery of the femur. CASE REPORT: A 7-year-old boy (29 kg) was scheduled for hip rotationplasty for resection of an osteosarcoma of the left femur under general anesthesia and postoperative pain therapy with an epidural stimulating catheter. In hip rotationplasty the femur is resected, the lower limb and foot are rotated 180 degrees and the tibia plateau is attached to the pelvic acetabulum to form a new hip joint. During preparation of the left thigh and the sciatic nerve, motor responses to stimulation of the catheter were preserved, but the stimulation threshold increased. After vascular anastomosis the foot remained cold, therefore ropivacaine was applied epidurally and subsequently a warming of the foot was observed. At the end of the operation, the patient was free of pain, a good capillary pulse of the leg was observed, and the patient was able to move the foot and toes of the rotated leg. CONCLUSIONS: The use of epidural stimulating catheters as a tool to monitor nerve function is a novel and simple procedure to monitor nerve function intraoperatively and to enable good postoperative pain control.