4-羟基-4′-联苯腈的合成

在三氯化铝催化下 ,以 4-羟基联苯为原料 ,与三氯乙酰氯进行酰基化反应合成了 4-羟基 - 4′-三氯乙酰基联苯 ,经氨解反应得到 4-羟基 - 4′-联苯甲酰胺 ,进而用氯化亚砜脱水制得 4-羟基 - 4′-联苯腈 ,总收率达 5 6% ,纯度≥ 99%。     

微生物方法脱除柴油中有机硫的初步研究

从土壤中分离筛选出l株可将二苯并噻吩代谢为2-羟基二苯和水溶性硫酸盐的细菌YC-LI-1。初步鉴定其属于假单胞菌属(Pseudomonas)，该菌株在水中对二苯并噻吩的转化率达到了90％以上。用模拟化合物二苯并噻吩和正十六烷考察了YC-LI-l脱除二苯并噻吩有机硫的最佳条件。并在该最佳条件下，对实际加氢柴油进行脱硫试验。结果表明，YC-LI-l菌株可脱除柴油中约50％的二苯并噻吩类有机硫。     

Altering 2‐Hydroxybiphenyl 3‐Monooxygenase Regioselectivity by Protein Engineering for the Production of a New Antioxidant

2‐Hydroxybiphenyl 3‐monooxygenase is a flavin‐containing NADH‐dependent aromatic hydroxylase that oxidizes a broad range of 2‐substituted phenols. In order to modulate its activity and selectivity, several residues in the active site pocket were investigated by saturation mutagenesis. Variant M321A demonstrated altered regioselectivity by oxidizing 3‐hydroxybiphenyl for the first time, thus enabling the production of a new antioxidant, 3,4‐dihydroxybiphenyl, with similar ferric reducing capacity to the well‐studied piceatannol. The crystal structure of M321A was determined (2.78 Å), and molecular docking of the 3‐substituted phenol provided a rational explanation for the altered regioselectivity. Furthermore, HbpA was found to possess pro‐S enantioselectivity towards the production of several chiral sulfoxides, whereas variant M321F exhibited improved enantioselectivity. Based on the biochemical characterization of several mutants, it was suggested that Trp97 stabilized the substrate in the active site, Met223 was involved in NADH entrance or binding to the active site, and Pro320 might facilitate FAD movement.